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Submit ReviewTempol
Description: Superoxide scavenger; antioxidant
Chemical Name: 1-Oxyl-2,2,6,6-tetramethyl-4-hydroxypiperidine
Purity: ≥99%
Biological Activity for Tempol
Tempol is a superoxide scavenger that displays neuroprotective, anti-inflammatory and analgesic effects. Tempol reduces γ-radiation-induced damage in hematopoietic stem cells in vitro and in vivo.
Technical Data for Tempol
| M. Wt | 172.24 |
| Formula | C9H18NO2 |
| Storage | Store at -20°C |
| Purity | ≥99% |
| CAS Number | 2226-96-2 |
| PubChem ID | 137994 |
| InChI Key | UZFMOKQJFYMBGY-UHFFFAOYSA-N |
| Smiles | CC1(C)CC(O)CC(C)(C)N1[O] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Tempol
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 17.22 | 100 | |
| ethanol | 17.22 | 100 |
Preparing Stock Solutions for Tempol
The following data is based on the product molecular weight 172.24. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 5.81 mL | 29.03 mL | 58.06 mL |
| 5 mM | 1.16 mL | 5.81 mL | 11.61 mL |
| 10 mM | 0.58 mL | 2.9 mL | 5.81 mL |
| 50 mM | 0.12 mL | 0.58 mL | 1.16 mL |
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Product Datasheets for Tempol
References for Tempol
References are publications that support the biological activity of the product.
Francis and Sehgal (2006) The superoxide scavenger TEMPOL induces urok. receptor (uPAR) expression in human prostate cancer cells. Mol.Cancer 5 21 PMID: 16756681
Deng-Bryant et al (2008) Neuroprotective effect of tempol, a catalytic scavenger of peroxynitrite-derived free radicals, in a mouse traumatic brain injury model. J.Cereb.Blood Flow Metab. 28 1114 PMID: 18319733
Khattab (2006) TEMPOL, a membrane-permeable radical scavenger, attenuates peroxynitrite- and superoxide anion-enhanced carrageenan-induced paw edema and hyperalgesia: a key role for superoxide anion. EurJ.Pharmacol. 548 167 PMID: 16973155
Ramachandran et al (2012) Prevention of γ-radiation induced cellular genotoxicity by tempol: Protection of hematopoietic system. Environ.Toxicol.Pharmacol. 34 253 PMID: 22609778
If you know of a relevant reference for Tempol, please let us know.
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Keywords: Tempol, Tempol supplier, Superoxide, scavenger, antioxidant, hematopoietic, haematopoietic, stem, cells, HSC, Antioxidants, Hematopoietic, Stem, Cells, 3082, Tocris Bioscience
9 Citations for Tempol
Citations are publications that use Tocris products. Selected citations for Tempol include:
Valente et al (2012) Angiotensin II enhances AT1-Nox1 binding and stimulates arterial smooth muscle cell migration and proliferation through AT1, Nox1, and interleukin-18. Am J Physiol Heart Circ Physiol 303 H282 PMID: 22636674
Somanna et al (2016) The Nox1/4 Dual Inhibitor GKT137831 or Nox4 Knockdown Inhibits Angiotensin-II-Induced Adult Mouse Cardiac Fibroblast Proliferation and Migration. AT1 Physically Associates With Nox4. J Cell Physiol 231 1130 PMID: 26445208
Hood et al (2016) Nicotinamide Adenine Dinucleotide Phosphate Oxidase-Mediated Redox Signaling and Vascular Remodeling by 16α-Hydroxyestrone in Human Pulmonary Artery Cells: Implications in Pulmonary Arterial Hypertension. Hypertension 68 796 PMID: 27402919
Ruparel et al (2012) Plasticity of cytochrome P450 isozyme expression in rat trigeminal ganglia neurons during inflammation. J Biol Chem 153 2031 PMID: 22633978
Tomasovic et al (2015) Sestrin 2 Regulates PDGF Receptor β (PDGFRβ) Expression by Modulating Proteasomal and Nrf2 Transcription Factor Functions. Brain Res NULL PMID: 25716320
Senturk et al (2014) p53ψ is a transcriptionally inactive p53 isoform able to reprogram cells toward a metastatic-like state. Proc Natl Acad Sci U S A 111 E3287 PMID: 25074920
Rastogi et al (2021) Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF PLoS Pathog 17 e1009712 PMID: 34324582
Beggs et al (2015) Trovafloxacin-induced replication stress sensitizes HepG2 cells to tumor necrosis factor-alpha-induced cytotoxicity mediated by extracellular signal-regulated kinase and ataxia telangiectasia and Rad3-related. Toxicology 331 35 PMID: 25748550
Nesuashvili et al (2013) Sensory nerve terminal mitochondrial dysfunction activates airway sensory nerves via transient receptor potential (TRP) channels. Mol Pharmacol 83 1007 PMID: 23444014
Do you know of a great paper that uses Tempol from Tocris? Please let us know.
Reviews for Tempol
Average Rating: 5 (Based on 1 Review.)
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Tempol blunts 15d-PGJ2-induced ROS levels in HL-1 cardiomyocytes.
By
Chintan Koyani on 10/13/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: HL-1 cardiomyocytes
HL-1 cardiomyocytes were incubated with Tempol (1 mM) for 30 min prior to 15d-PGJ2 treatments (15 μM) for 1 h to follow ROS generation using carboxy-H2DCFDA (10 μM). Figure shows that the antioxidant Tempol (a cell-permeable superoxide anion scavenger) blunted 15d-PGJ2-induced ROS formation efficiently.
