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Submit ReviewActinomycin D
Description: Inhibits RNA polymerase
Chemical Name: 2-Amino-(N,N)-1-bis(hexadecahydro-6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxo-1H-pyrrolo[2,1]-[1,4,7,10,13] oxatetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide
Purity: ≥95% (HPLC)
Biological Activity for Actinomycin D
Actinomycin D is an anti-neoplastic antibiotic. Inhibits RNA polymerase and is a potent inducer of apoptosis.
Technical Data for Actinomycin D
| M. Wt | 1255.43 |
| Formula | C62H86N12O16 |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 50-76-0 |
| PubChem ID | 2019 |
| InChI Key | RJURFGZVJUQBHK-DCUCITLNSA-N |
| Smiles | [H][C@@]12CCCN1C([C@H](C(C)C)NC([C@H]([C@H](OC([C@@H](N(C(CN(C2=O)C)=O)C)C(C)C)=O)C)NC(C3=C4N=C5C(OC4=C(C=C3)C)=C(C(C(N)=C5C(N[C@H]6[C@H](OC([C@@H](N(C(CN(C([C@@]7(CCCN7C([C@H](C(C)C)NC6=O)=O)[H])=O)C)=O)C)C(C)C)=O)C)=O)=O)C)=O)=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Actinomycin D
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 62.77 | 50 |
Preparing Stock Solutions for Actinomycin D
The following data is based on the product molecular weight 1255.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 1.59 mL | 7.97 mL | 15.93 mL |
| 2.5 mM | 0.32 mL | 1.59 mL | 3.19 mL |
| 5 mM | 0.16 mL | 0.8 mL | 1.59 mL |
| 25 mM | 0.03 mL | 0.16 mL | 0.32 mL |
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Product Datasheets for Actinomycin D
References for Actinomycin D
References are publications that support the biological activity of the product.
Aktipis et al (1981) A kinetic study on the mechanism of inhibition of RNA synthesis catalyzed by DNA-dependent RNA polymerase. Differences in inhibition by ethidium bromide, 3,8-diamino-6-ethylphenanthridinium bromide and actinomycin D. Biochim.Biophys.Acta 655 278 PMID: 7025910
Glynn et al (1992) Apoptosis induced by actinomycin D, camptothecin or aphidicolin can occur in all phases of the cell cycle. Biochem.Soc.Trans. 20 84S PMID: 1634006
Jeeninga et al (1998) The mechanism of actinomycin D-mediated inhibition of HIV-1 reverse transcription. Nucleic Acids Res. 26 5472 PMID: 9826774
If you know of a relevant reference for Actinomycin D, please let us know.
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Keywords: Actinomycin D, Actinomycin D supplier, RNA, polymerases, inhibitors, inhibits, Antineoplastic, antibiotics, Apoptosis, Inducers, ActinomycinD, DNA,, and, Protein, Synthesis, Antibiotics, Polymerase, 1229, Tocris Bioscience
⚠ WARNING: This product can expose you to chemicals including Actinomycin D, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov
19 Citations for Actinomycin D
Citations are publications that use Tocris products. Selected citations for Actinomycin D include:
Livingstone et al (2019) Secreted Amyloid Precursor Protein-Alpha Promotes Arc Protein Synthesis in Hippocampal Neurons. Front Mol Neurosci 12 198 PMID: 31474829
Yuan and Burrell (2013) Endocannabinoid-dependent long-term depression in a nociceptive synapse requires coordinated presynaptic and postsynaptic transcription and translation. J Neurosci 33 4349 PMID: 23467351
Huang et al (2010) Pharmacological and genetic accumulation of hypoxia-inducible factor-1alpha enhances excitatory synaptic transmission in hippocampal neurons through the production of vascular endothelial growth factor. J Neurosci 30 6080 PMID: 20427666
Di et al (2016) Acute Stress Suppresses Synaptic Inhibition and Increases Anxiety via Endocannabinoid Release in the Basolateral Amygdala. J.Neurosci. 36 8461 PMID: 27511017
Kang et al (2012) Loss of neuron-astroglial interaction rapidly induces protective CNTF expression after stroke in mice. J Neurosci 32 9277 PMID: 22764235
Tian et al (2010) MEF-2 regulates activity-dependent spine loss in striatopallidal medium spiny neurons. Mol Cell Neurosci 44 94 PMID: 20197093
Visavadiya (2016) Integrin-FAK signaling rapidly and potently promotes mitochondrial function through STAT3. Cell Communication and Signaling 14 32 PMID: 27978828
Parker and Kooi (2014) Microplate-based screening for small molecule inhibitors of neuropilin-2/vascular endothelial growth factor-C interactions. J Biol Chem 453 42525 PMID: 24583243
Cheang et al (2011) Endothelial nitric oxide synthase enhancer reduces oxidative stress and restores endothelial function in db/db mice. Biochem Biophys Res Commun 92 267 PMID: 21875904
Wang et al (2014) SynGAP regulates protein synthesis and homeostatic synaptic plasticity in developing cortical networks. PLoS One 8 e83941 PMID: 24391850
Gutierrez et al (2014) The anticancer agent di-2-pyridylketone 4,4-dimethyl-3-thiosemicarbazone (Dp44mT) overcomes prosurvival autophagy by two mechanisms: persistent induction of autophagosome synthesis and impairment of lysosomal integrity. J Biol Chem 289 33568 PMID: 25301941
Khattar et al (2019) Structural determinants and genetic modifications enhance BMP2 stability and extracellular secretion. FASEB Bioadv 1 180 PMID: 31225515
Podszywalow-Bartnicka et al (2014) Downregulation of BRCA1 protein in BCR-ABL1 leukemia cells depends on stress-triggered TIAR-mediated suppression of translation. Cardiovasc Res 13 3727 PMID: 25483082
Wong et al (2009) Cyclooxygenase-2-derived prostaglandin F2alpha mediates endothelium-dependent contractions in the aortae of hamsters with increased impact during aging. Circ Res 104 228 PMID: 19096033
Backlund et al (2009) Posttranscriptional regulation of angiotensin II type 1 receptor expression by glyceraldehyde 3-phosphate dehydrogenase. Nucleic Acids Res 37 2346 PMID: 19246543
Thomson et al (2017) Cell-Type-Specific Translation Profiling Reveals a Novel Strategy for Treating Fragile X Syndrome. Neuron 95 550 PMID: 28772121
Chan et al (2007) Heat shock protein 60 or 70 activates nitric-oxide synthase (NOS) I- and inhibits NOS II-associated signaling and depresses the mitochondrial apoptotic cascade during brain stem death. Cell Cycle 282 4585 PMID: 17150954
Vecsey et al (2007) Histone deacetylase inhibitors enhance memory and synaptic plasticity via CREB:CBP-dependent transcriptional activation. J Neurosci 27 6128 PMID: 17553985
Paukku et al (2008) p100 increases AT1R expression through interaction with AT1R 3'-UTR. Nucleic Acids Res 36 4474 PMID: 18603592
Do you know of a great paper that uses Actinomycin D from Tocris? Please let us know.
Reviews for Actinomycin D
Average Rating: 5 (Based on 2 Reviews.)
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Mitochondrial bioenergetics measurements by reagent screen.
By
Anonymous on 03/15/2020
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: mouse brain endothelioma cell line
0.3 μg/ml
In vivo assay.
By
Anonymous on 01/07/2018
Assay Type: In Vitro
Species: Human
I used in my experiment for muHL6b cells with 0.2 μg/ml actinomycin D final concentration.