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Submit ReviewDMSO, sterile filtered
Description: DMSO, sterile filtered, wide ranging applications in cell culture, cryopreservation and organic chemistry. Solvent for polar and nonpolar compounds
Alternative Names: Methyl sulfoxide
Chemical Name: Dimethyl sulfoxide
Purity: ≥99.9%
Biological Activity for DMSO, sterile filtered
DMSO, sterile filtered is a solvent with wide ranging applications in cell culture, cryopreservation and organic chemistry. DMSO dissolves both polar and nonpolar compounds. Improves responsiveness of human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) to differentiation signals. DMSO is used in PCR to inhibit secondary structure formation by DNA template or primers.
DMSO, Cell Cryopreserve Grade (Cat. No. 7726) also available.
Technical Data for DMSO, sterile filtered
| M. Wt | 78.13 |
| Formula | C2H6OS |
| Storage | Store at RT |
| Purity | ≥99.9% |
| CAS Number | 67-68-5 |
| PubChem ID | 679 |
| InChI Key | IAZDPXIOMUYVGZ-UHFFFAOYSA-N |
| Smiles | CS(C)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for DMSO, sterile filtered
References for DMSO, sterile filtered
References are publications that support the biological activity of the product.
Chetty et al (2013) A simple tool to improve pluripotent stem cell differentiation. Nat.Methods 10 553 PMID: 23584186
If you know of a relevant reference for DMSO, sterile filtered, please let us know.
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Keywords: DMSO, sterile filtered, DMSO, sterile filtered supplier, Solvent, Reagents, Methyl, sulfoxide, Biochemicals, and, Molecular, Biology, ESCs, iPSC, Buffers,, Solvents, Solutions, Loop-mediated, Isothermal, Amplification, 3176, Tocris Bioscience
16 Citations for DMSO, sterile filtered
Citations are publications that use Tocris products. Selected citations for DMSO, sterile filtered include:
Hermanson et al (2012) Screening for novel LRRK2 inhibitors using a high-throughput TR-FRET cellular assay for LRRK2 Ser935 phosphorylation. J Neuroendocrinol 7 e43580 PMID: 22952710
Ho et al (2012) Photoperiodic changes in endocannabinoid levels and energetic responses to altered signalling at CB1 receptors in Siberian hamsters. Behav Brain Res 24 1030 PMID: 22420341
Zechel et al (2016) Thalamo-cortical axons regulate the radial dispersion of neocortical GABAergic interneurons. Elife 5 PMID: 27935475
Gofman et al (2016) Relative Role of Akt, ERK and CREB in Alcohol-Induced Microglia P2X4R Receptor Expression. Alcohol Alcohol 51 647 PMID: 26946194
Ito et al (2015) Influenza induces IL-8 and GM-CSF secretion by human alveolar epithelial cells through HGF/c-Met and TGF-α/EGFR signaling. Mol Brain 308 L1178 PMID: 26033355
Obis et al (2015) The novel protein kinase C ε isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release. PLoS One 8 8 PMID: 25761522
Obis et al (2015) The novel protein kinase C ε isoform modulates acetylcholine release in the rat neuromuscular junction. J Neurophysiol 8 80 PMID: 26625935
Ji et al (2014) L-type Ca2+ channel responses to bay k 8644 in stem cell-derived cardiomyocytes are unusually dependent on holding potential and charge carrier. Assay Drug Dev Technol 12 352 PMID: 25147907
Chen (2014) The development of zebrafish tendon and ligament progenitors. Development 141 2035 PMID: 24803652
Lively et al (2018) Comparing Effects of Transforming Growth Factor β1 on Microglia From Rat and Mouse: Transcriptional Profiles and Potassium Channels. Front Cell Neurosci 12 115 PMID: 29780305
Morizane and Bonventre (2017) Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells. Nat Protoc 12 195 PMID: 28005067
Wagner et al (2013) The dorsomedial hypothalamus mediates stress-induced hyperalgesia and is the source of the pronociceptive peptide cholecystokinin in the rostral ventromedial medulla. Am J Physiol Lung Cell Mol Physiol 238 29 PMID: 23415792
Hughes et al (2013) Endogenous analgesic action of the pontospinal noradrenergic system spatially restricts and temporally delays the progression of neuropathic pain following tibial nerve injury. Mol Brain 154 1680 PMID: 23707289
Olkhanud et al (2011) Tumor-evoked regulatory B cells promote breast cancer metastasis by converting resting CD4+ T cells to T-regulatory cells. Cancer Res 71 3505 PMID: 21444674
Romo-Parra et al (2008) GABA actions in hippocampal area CA3 during postnatal development: differential shift from depolarizing to hyperpolarizing in somatic and dendritic compartments. Neuroscience 99 1523 PMID: 18216229
Andäng et al (2008) Histone H2AX-dependent GABA(A) receptor regulation of stem cell proliferation. Nature 451 460 PMID: 18185516
Do you know of a great paper that uses DMSO, sterile filtered from Tocris? Please let us know.
Reviews for DMSO, sterile filtered
Average Rating: 5 (Based on 3 Reviews.)
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Filter by:
In vivo use.
By
Anonymous on 08/14/2023
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: colorectal cancer cells
I used this DMSO for in vivo mice injections. The product worked very well in vivo. 10% DMSO solution was prepared in sterile PBS and the mice were injected with 200-300 uL i.p. every other day. All mice tolerated the injection and survived very well for more than 4 weeks.
DMSO for cryopreservation of HBECs.
By
Angie Sin on 01/25/2021
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HBEC-5i (ATCC CRL-3245)
We use DMSO for cryopreservation of HBECs - 92.5% of complete growth medium (containing DMEM/F12, 10% FBS, 1% penicillin-streptomycin and 40 µg/mL endothelial growth supplement) : 7.5% of DMSO. Cells grow well after thawing.
To dissolve CHIR for kidney differentiation from iPSC.
By
Pinyuan Tian on 08/07/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: iPSC
DMSO is used for dissolving CHIR (4423).Reconstitute 10 mg of CHIR in 2.149 ml of DMSO for a final concentration of 10mM.
Make 100µl aliquots and keep them at −80 °C for 6-12 months.
