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CCCP

Cat. No. 0452 4 Citations 1 Review Submit a Review Datasheet / COA / SDS
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Description: Oxidative phosphorylation uncoupler
Chemical Name: Carbonyl cyanide 3-chlorophenylhydrazone
Purity: ≥98% (HPLC)
Datasheet
Citations (4)
Reviews (1)
Literature (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for CCCP

CCCP is a protonophore. It disrupts mitochondria membrane potential by transporting protons across the mitochondrial inner membrane, which interferes with the proton gradient and ATP synthesis. CCCP is a widely used as an uncoupler of oxidative phosphorylation to induce mitochondrial depolarization.

Technical Data for CCCP

M. Wt 204.62
Formula C9H5ClN4
Storage Store at RT
Purity ≥98% (HPLC)
CAS Number 555-60-2
PubChem ID 2603
InChI Key UGTJLJZQQFGTJD-UHFFFAOYSA-N
Smiles ClC1=CC=CC(N/N=C(C#N)\C#N)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for CCCP

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 20.46 100

Preparing Stock Solutions for CCCP

The following data is based on the product molecular weight 204.62. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 4.89 mL 24.44 mL 48.87 mL
5 mM 0.98 mL 4.89 mL 9.77 mL
10 mM 0.49 mL 2.44 mL 4.89 mL
50 mM 0.1 mL 0.49 mL 0.98 mL

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Product Datasheets for CCCP

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for CCCP

References are publications that support the biological activity of the product.

Heytler and Pritchard (1962) A new class of uncoupling agents - carbonyl cyanide phenylhydrazones. Biochem.Biophys.Res.Commun. 7 272 PMID: 13907155

If you know of a relevant reference for CCCP, please let us know.

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Keywords: CCCP, CCCP supplier, Oxidative, phosphorylation, uncoupler, protonophore, proton, gradient, mitochondrial, depolarization, Phosphorylation, 0452, Tocris Bioscience

4 Citations for CCCP

Citations are publications that use Tocris products. Selected citations for CCCP include:

Choubey et al (2014) BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria. Autophagy 10 1105 PMID: 24879156

Purnell (2014) Efavirenz induces neuronal autophagy and mitochondrial alterations. J Pharmacol Exp Ther 351 250 PMID: 25161171

Chouhan et al (2010) Presynaptic mitochondria in functionally different motor neurons exhibit similar affinities for Ca2+ but exert little influence as Ca2+ buffers at nerve firing rates in situ. J Neurosci 30 1869 PMID: 20130196

Guo et al (2015) Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPSC-CMs) to Monitor Compound Effects on Cardiac Myocyte Signaling Pathways. Curr Protoc Chem Biol 7 141 PMID: 26331525

Do you know of a great paper that uses CCCP from Tocris? Please let us know.

Reviews for CCCP

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Mitochondrial studies in live neurons.
By Karthik krishnamurthy on 10/24/2018
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: spinal cord neurons

TMRM fluorescence intensity before and after CCCP addition was used for the quantitation of change in mitochondrial membrane potential in spinal cord neurons induced by glutamate receptor agonist AMPA

Time lapse images of TMRM fluorescence of mitochondria rich regions in neurons was recorded. The fluorescence intensity (F) at a given time point was normalized to the initial fluorescence intensity (F0) before addition of the AMPA. At the end of each experiment CCCP (750 nM) was added to the bath. CCCP collapses the mitochondrial membrane potential and decreases the TMRM fluorescence.

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Literature in this Area

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