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Description: Versatile marker for neuroanatomical investigations
Chemical Name: N6-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine
Biological Activity for Biocytin
Biocytin is a versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.
Technical Data for Biocytin
| M. Wt | 372.48 |
| Formula | C16H28N4O4S |
| Storage | Store at -20°C |
| CAS Number | 576-19-2 |
| PubChem ID | 83814 |
| InChI Key | BAQMYDQNMFBZNA-MNXVOIDGSA-N |
| Smiles | O=C1N[C@@]2([H])[C@@](CS[C@H]2CCCCC(NCCCC[C@H](N)C(O)=O)=O)([H])N1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Biocytin
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 18.62 | 50 with gentle warming |
Preparing Stock Solutions for Biocytin
The following data is based on the product molecular weight 372.48. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 5.37 mL | 26.85 mL | 53.69 mL |
| 2.5 mM | 1.07 mL | 5.37 mL | 10.74 mL |
| 5 mM | 0.54 mL | 2.68 mL | 5.37 mL |
| 25 mM | 0.11 mL | 0.54 mL | 1.07 mL |
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Product Datasheets for Biocytin
References for Biocytin
References are publications that support the biological activity of the product.
Kobbert et al (2000) Current concepts in neuroanatomical tracing. Prog.Neurobiol. 62 327 PMID: 10856608
Roth et al (2004) Morphology and axonal projection pattern of neurons in the telencephalon of the fire-bellied toad Bombina orientalis: an anterograde, retrograde and intracellular biocytin labelling study. J.Comp.Neurol. 478 35 PMID: 15334648
MacNeil and Gaul (2008) Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones. J.Comp.Neurol. 506 6 PMID: 17990268
If you know of a relevant reference for Biocytin, please let us know.
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Keywords: Biocytin, Biocytin supplier, Versatile, marker, neuroanatomical, investigations, reagents, anterograde, retrograde, Biochemicals, and, Molecular, Biology, 3349, Tocris Bioscience
12 Citations for Biocytin
Citations are publications that use Tocris products. Selected citations for Biocytin include:
Roesler et al (2019) Myosin XVI Regulates Actin Cytoskeleton Dynamics in Dendritic Spines of Purkinje Cells and Affects Presynaptic Organization. Front Cell Neurosci 13 330 PMID: 31474830
Ferrarese et al (2018) Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo. Cell Rep 24 3455 PMID: 30257207
Tsai et al (2012) Autistic-like behaviour and cerebellar dysfunction in Purkinje cell Tsc1 mutant mice. MBio 488 647 PMID: 22763451
Xia et al (2014) Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons. PLoS One 9 e100968 PMID: 24971996
Cunningham et al (2014) hPSC-derived maturing GABAergic interneurons ameliorate seizures and abnormal behavior in epileptic mice. Cell Stem Cell 15 559 PMID: 25517465
Dettmann et al (2014) Isolation, expansion and transplantation of postnatal murine progenitor cells of the enteric nervous system. PLoS One 9 e97792 PMID: 24871092
Lam et al (2011) Leptin does not directly affect CNS serotonin neurons to influence appetite. Acta Crystallogr F Struct Biol Commun 13 584 PMID: 21531340
Estakhr et al (2017) Differential Control of Dopaminergic Excitability and Locomotion by Cholinergic Inputs in Mouse Substantia Nigra. Curr Biol 27 1900 PMID: 28648825
Kim and Cho (2017) Encoding of Discriminative Fear Memory by Input-Specific LTP in the Amygdala. Neuron 95 1129 PMID: 28823727
Chai et al (2017) Neural Circuit-Specialized Astrocytes: Transcriptomic, Proteomic, Morphological, and Functional Evidence. Neuron 95 531 PMID: 28712653
Jerome et al (2011) Parallel optical control of spatiotemporal neuronal spike activity using high-speed digital light processing. Front Syst Neurosci 5 70 PMID: 21904526
Robinson and Wyatt (2015) Identification and structure solution of fragment hits against kinetoplastid N-myristoyltransferase. Nature 71 586 PMID: 25945713
Do you know of a great paper that uses Biocytin from Tocris? Please let us know.
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