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Description: O-GlcNAcase and β-hexosaminidase inhibitor
Chemical Name: O-(2-Acetamido-2-deoxy-D-glucopyranosylidene)amino-Z-N-phenylcarbamate
Purity: ≥98% (HPLC)
Biological Activity for (Z)-Pugnac
(Z)-Pugnac is an O-GlcNAc-β-N-acetylglucosaminidase (O-GlcNAcase) and β-hexosaminidase inhibitor (Ki values are 46 and 36 nM respectively) that increases O-GlcNAc levels ~ 2-fold in HT29 cells. Z-linked isomer is more potent than the E isomer.
Technical Data for (Z)-Pugnac
| M. Wt | 353.33 |
| Formula | C15H19N3O7 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 132489-69-1 |
| PubChem ID | 6074876 |
| InChI Key | PBLNJFVQMUMOJY-JXAWBTAJSA-N |
| Smiles | OC(C1NC(C)=O)C(O)C(CO)O/C1=N\OC(NC2=CC=CC=C2)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for (Z)-Pugnac
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 35.33 | 100 |
Preparing Stock Solutions for (Z)-Pugnac
The following data is based on the product molecular weight 353.33. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.83 mL | 14.15 mL | 28.3 mL |
| 5 mM | 0.57 mL | 2.83 mL | 5.66 mL |
| 10 mM | 0.28 mL | 1.42 mL | 2.83 mL |
| 50 mM | 0.06 mL | 0.28 mL | 0.57 mL |
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Product Datasheets for (Z)-Pugnac
References for (Z)-Pugnac
References are publications that support the biological activity of the product.
Dong and Hart (1994) Purification and characterization of an O-GlcNAc selective N-acetyl-β-D-glucosaminidase from rat spleen cytosol. J.Biol.Chem. 269 19321 PMID: 8034696
Macauley et al (2005) O-GlcNAcase uses substrate-assisted catalysis. J.Biol.Chem. 280 25313 PMID: 15795231
Perreira et al (2006) Inhibition of O-GlcNAcase by PUGNAc is dependent upon the oxime stereochemistry. Bioorg.Med.Chem. 14 837 PMID: 16214344
If you know of a relevant reference for (Z)-Pugnac, please let us know.
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Keywords: Pugnac, Pugnac supplier, O-GlcNAcase, β-hexosaminidase, beta-hexosaminidase, O-GlcNAc-β-N-acetylglucosaminidase, O-GlcNAc-beta-N-acetylglucosaminidase, inhibitors, inhibits, Hydrolases, Other, 3384, Tocris Bioscience
2 Citations for (Z)-Pugnac
Citations are publications that use Tocris products. Selected citations for (Z)-Pugnac include:
Jo et al (2016) O-GlcNAcylation of ATG4B positively regulates autophagy by increasing its hydroxylase activity. Oncotarget 7 57186 PMID: 27527864
Parween et al (2018) Higher O-GlcNAc Levels Are Associated with Defects in Progenitor Proliferation and Premature Neuronal Differentiation during in-Vitro Human Embryonic Cortical Neurogenesis. Front Cell Neurosci 11 415 PMID: 29311838
Do you know of a great paper that uses (Z)-Pugnac from Tocris? Please let us know.
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