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Submit ReviewCalyculin A
Description: Protein phosphatase 1 and 2A inhibitor
Chemical Name: N-[(3S)-[4-(1E)-3-[(2R,3R,4R,7S,8S,9R)-2-[(1S,3S,4S,5R,7E,9E,11E,13Z)-14-Cyano-3,5-dihydroxy-1-methoxy-4,6,8,9,13-pentamethyl-7,9,11,13-tetradecatetraenyl]-9-hydroxy-4,4,8-trimethyl-3-(phosphonooxy)-1,6-dioxaspiro[4.5]dec-7-yl]-1-propenyl]-2-oxazoly]butyl]-4-deoxy-4-(dimethylamino)-5-O-methyl-L-ribonamide
Purity: ≥97% (HPLC)
Biological Activity for Calyculin A
Calyculin A is a potent and selective cell-permeable inhibitor of protein phosphatase 1 (IC50 = 0.3 - 0.7 nM) and protein phosphatase 2A (IC50 = 0.5 - 1 nM). Displays > 10,000,000-fold selectivity over PP2B and PP2C.
Note: This product is typically prepared in DMSO.
Technical Data for Calyculin A
| M. Wt | 1009.18 |
| Formula | C50H81N4O15P |
| Storage | Store at -20°C |
| Purity | ≥97% (HPLC) |
| CAS Number | 101932-71-2 |
| PubChem ID | 3894 |
| InChI Key | ULTTYPMRMMDONC-UHFFFAOYSA-N |
| Smiles | O[C@H]1[C@H](C)[C@H](C/C=C/C3=COC([C@@H](C)CCNC([C@H]([C@H]([C@@H](N(C)C)COC)O)O)=O)=N3)O[C@]2([C@](C)(C)[C@@H](OP(O)(O)=O)[C@]([C@@H](OC)C[C@H](O)[C@H](C)[C@H](O)[C@H](C)/C=C(C)/C(C)=C/C=C/C(C)=C\C#N)([H])O2)C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Calyculin A
References for Calyculin A
References are publications that support the biological activity of the product.
Favre et al (1997) Differential inhibition and posttranslational modification of protein phosphatase 1 and 2A in MCF7 cells treated with calyculin-A, okadaic acid, and tautomycin. J.Biol.Chem. 272 13856 PMID: 9153244
Ishihara et al (1989) Calyculin A and okadaic acid: inhibitors of protein phosphatase activity. Biochem.Biophys.Res.Commun. 159 871 PMID: 2539153
Lindval et al (1997) The binding mode of calyculin A to protein phosphatase-1. J.Biol.Chem. 272 23312 PMID: 9287341
McCluskey et al (2002) Serine-threonine protein phosphatase inhibitors: development of therapeutic strategies. J.Med.Chem. 45 1151 PMID: 11881984
If you know of a relevant reference for Calyculin A, please let us know.
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Keywords: Calyculin A, Calyculin A supplier, Protein, phosphatase, 1, 2A, inhibitors, inhibits, Calcineurin, Ser/Thr, Phosphatases, PP1, PP2A, CalyculinA, 1336, Tocris Bioscience
9 Citations for Calyculin A
Citations are publications that use Tocris products. Selected citations for Calyculin A include:
Chiu et al (2019) NMDAR-Activated PP1 Dephosphorylates GluN2B to Modulate NMDAR Synaptic Content. Cell Rep 28 332 PMID: 31291571
Colpaert et al (2005) Nitric oxide relaxes circular smooth muscle of rat distal colon through RhoA/Rho-kinase independent Ca2+ desensitisation. Br J Pharmacol 144 588 PMID: 15655498
Bhattachariya et al (2014) PYK2 selectively mediates signals for growth versus differentiation in response to stretch of spontaneously active vascular smooth muscle. Physiol Rep 2 PMID: 25347863
Kii et al (2016) Selective inhibition of the kinase DYRK1A by targeting its folding process. Nat Commun 7 11391 PMID: 27102360
Isensee et al (2014) Pain modulators regulate the dynamics of PKA-RII phosphorylation in subgroups of sensory neurons. J Cell Sci 127 216 PMID: 24190886
Fabbrizi et al (2018) Transient PP2A inhibition alleviates normal tissue stem cell susceptibility to cell death during radiotherapy. Cell Death Dis 9 492 PMID: 29706648
Burdyga et al (2018) Phosphatases control PKA-dependent functional microdomains at the outer mitochondrial membrane. Proc Natl Acad Sci U S A. 115 E6497 PMID: 29941564
Liu (2018) In vivo brain GPCR signaling elucidated by phosphoproteomics. Science 360 eaao4927 PMID: 29930108
Chazeau et al (2015) Mechanical coupling between transsynaptic N-cadherin adhesions and actin flow stabilizes dendritic spines. J Neurochem 26 859 PMID: 25568337
Do you know of a great paper that uses Calyculin A from Tocris? Please let us know.
Reviews for Calyculin A
Average Rating: 5 (Based on 1 Review.)
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Product performed ideally over years and would buy again..
By
Anonymous on 04/04/2020
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: Sprague-Dawley rats primary neuronal
calyculin A for PP1/PP2A (Cal A, 100 nM)
