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Description: Induces definitive endoderm formation in mouse and human ESCs
Chemical Name: Heptanedioic acid-1-(2-cyclopentylidenehydrazide)
Purity: ≥98% (HPLC)
Biological Activity for IDE 2
IDE 2 is a cell-permeable inducer of definitive endoderm formation in mouse and human embryonic stem cells (ESCs) (EC50 = 223 nM for induction of Sox17 expression in ESCs). Reported to activate TGF-β signaling and downstream Smad2 phosphorylation; upregulates Nodal expression. Promotes pancreatic progenitor cell formation in vitro, and gut tube formation in vivo.
Licensing Information
Sold for research purpose under license from Harvard University.
Technical Data for IDE 2
| M. Wt | 240.3 |
| Formula | C12H20N2O3 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1136466-93-7 |
| PubChem ID | 23836158 |
| InChI Key | CVYPYYPTFNVREL-UHFFFAOYSA-N |
| Smiles | O=C(N/N=C1/CCCC1)CCCCCC(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for IDE 2
References for IDE 2
References are publications that support the biological activity of the product.
Borowniak et al (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4 348 PMID: 19341624
Zaret (2009) Using small molecules to great effect in stem cell differentiation. Cell Stem Cell 4 373 PMID: 19427285
Filby et al (2011) Stimulation of AVNA/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells. Stem Cell Res.Ther. 2 16 PMID: 21463501
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Keywords: IDE 2, IDE 2 supplier, IDE2, inducer, induces, endodermal, formation, embryonic, stem, cells, differentiation, ESCs, nodal, sox17, and, iPSC, 4016, Tocris Bioscience
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Literature in this Area
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Stem Cell Research Product Guide
This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:
- Self-renewal and Maintenance
- Differentiation
- Reprogramming
- Organoid Generation
- GMP and Ancillary Material Grade Products
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.