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Submit Reviewα-Amanitin
Description: Inhibitor of RNA polymerase II
Chemical Name: cyclo[L-Asparaginyl-4-hydroxy-L-proly-(R-4,5-dihydroxy-L-isoleucyl-6-hydroxy-2-mercapto-L-tryptophylglycyl-L-isoleucylglycyl-L-cysteinyl]cyclic (4→8)-sulfide (R)-S-oxide
Purity: ≥95% (HPLC)
Biological Activity for α-Amanitin
α-Amanitin is an inhibitor of RNA polymerase II. Inhibits transcription in eukaryotic cells. Binds and blocks the largest subunits of RNA polymerase II, preventing new ribonucleotides from incorporating into the nascent RNA chain. Potent amatoxin.
Technical Data for α-Amanitin
| M. Wt | 918.97 |
| Formula | C39H54N10O14S |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 23109-05-9 |
| PubChem ID | 441541 |
| InChI Key | CIORWBWIBBPXCG-JAXJKTSHSA-N |
| Smiles | O=[C@@](N[C@@](C(N[C@@H](CC2=C(S4=O)NC3=C2C=CC(O)=C3)C(NCC(N[C@]([H])5[C@@H](C)CC)=O)=O)=O)([H])[C@@H](C)[C@@H](O)CO)[C@@H]1C[C@@H](O)CN1C([C@H](CC(N)=O)NC([C@H](C4)NC(CNC5=O)=O)=O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for α-Amanitin
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 4.59 | 5 |
Preparing Stock Solutions for α-Amanitin
The following data is based on the product molecular weight 918.97. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.05 mM | 21.76 mL | 108.82 mL | 217.63 mL |
| 0.25 mM | 4.35 mL | 21.76 mL | 43.53 mL |
| 0.5 mM | 2.18 mL | 10.88 mL | 21.76 mL |
| 2.5 mM | 0.44 mL | 2.18 mL | 4.35 mL |
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Product Datasheets for α-Amanitin
References for α-Amanitin
References are publications that support the biological activity of the product.
Casse et al (1999) The transcriptional inhibitors, actinomycin D and α-amanitin, activate the HIV-1 promoter and favor phosphorylation of the RNA polymerase II C-terminal domain. J.Biol.Chem. 274 16097 PMID: 10347161
Raha et al (2010) Close association of RNA polymerase II and many transcription factors with Pol III genes. Proc.Natl.Acad.Sci. 107 3639
If you know of a relevant reference for α-Amanitin, please let us know.
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Keywords: alpha-Amanitin, alpha-Amanitin supplier, alpha-Amanitin, a-Amanitin, α-Amanitin, RNA, polymerases, II, inhibitors, inhibits, DNA,, and, Protein, Synthesis, Polymerase, 4025, Tocris Bioscience
4 Citations for α-Amanitin
Citations are publications that use Tocris products. Selected citations for α-Amanitin include:
Philip R et al (2020) HIV-1 replication complexes accumulate in nuclear speckles and integrate into speckle-associated genomic domains. Nat Commun 11 3505 PMID: 32665593
Feng et al (2021) Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I. J Cell Biol 220 PMID: 34379093
Jing et al (2019) Remodeling of Interstrand Crosslink Proximal Replisomes Is Dependent on ATR, FANCM, and FANCD2. Cell Rep 27 1794-1808.e5 PMID: 31067464
Rafael et al (2022) BRD2 compartmentalizes the accessible genome. Nat Genet 54 481-491 PMID: 35410381
Do you know of a great paper that uses α-Amanitin from Tocris? Please let us know.
Reviews for α-Amanitin
Average Rating: 5 (Based on 1 Review.)
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alpha -Amanitin was used in antibacterial resistance experiments.
By
Anonymous on 02/20/2019
Species: Human
alpha -Amanitin was used in antibacterial resistance experiments.We looked into RNAP translocation along the DNA without dissociating from either the template or the growing RNA product until it encounters a termination factor or signal that causes the transcription cycle end with dissociation of the transcribing complex and release of RNA polymerase for a new round of transcription.
