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Submit ReviewPuromycin dihydrochloride
Description: Protein synthesis inhibitor
Chemical Name: 3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride
Purity: ≥98% (HPLC)
Biological Activity for Puromycin dihydrochloride
Puromycin dihydrochloride is a protein synthesis inhibitor; leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Aminonucleoside antibiotic. Inhibits translation in both in vitro and in vivo systems. Also inhibits the transport of proteins into the mitochondria in vitro.
Technical Data for Puromycin dihydrochloride
| M. Wt | 544.43 |
| Formula | C22H29N7O5.2HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 58-58-2 |
| PubChem ID | 443311 |
| InChI Key | MKSVFGKWZLUTTO-FZFAUISWSA-N |
| Smiles | O[C@@H]1[C@H](NC([C@@H](N)CC4=CC=C(OC)C=C4)=O)[C@@H](CO)O[C@H]1N3C2=NC=NC(N(C)C)=C2N=C3.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Puromycin dihydrochloride
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 54.44 | 100 | |
| DMSO | 54.44 | 100 |
Preparing Stock Solutions for Puromycin dihydrochloride
The following data is based on the product molecular weight 544.43. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.84 mL | 9.18 mL | 18.37 mL |
| 5 mM | 0.37 mL | 1.84 mL | 3.67 mL |
| 10 mM | 0.18 mL | 0.92 mL | 1.84 mL |
| 50 mM | 0.04 mL | 0.18 mL | 0.37 mL |
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Product Datasheets for Puromycin dihydrochloride
References for Puromycin dihydrochloride
References are publications that support the biological activity of the product.
Price and Verner (1993) Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction. Biochim.Biophys.Acta. 1150 89 PMID: 8334141
Azzam and Algranati (1973) Mechanism of puromycin action: fate of ribosomes after release of nascent polypeptide chains from polysomes. Proc.Nat.Acad.Sci. 70 3866 PMID: 4590173
If you know of a relevant reference for Puromycin dihydrochloride, please let us know.
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Keywords: Puromycin dihydrochloride, Puromycin dihydrochloride supplier, Puromycin, antibiotics, protein, synthesis, inhibitors, inhibits, translation, DNA,, RNA, and, Protein, Synthesis, Antibiotics, 4089, Tocris Bioscience
16 Citations for Puromycin dihydrochloride
Citations are publications that use Tocris products. Selected citations for Puromycin dihydrochloride include:
Dobrikov et al (2014) Mitotic phosphorylation of eukaryotic initiation factor 4G1 (eIF4G1) at Ser1232 by Cdk1:cyclin B inhibits eIF4A helicase complex binding with RNA. Mol Cell Biol 34 439 PMID: 24248602
Dasgupta et al (2014) Proteasome inhibitors alter levels of intracellular peptides in HEK293T and SH-SY5Y cells. PLoS One 9 e103604 PMID: 25079948
Zhu et al (2015) All-Trans Retinoic Acid-Induced Deficiency of the Wnt/β-Catenin Pathway Enhances Hepatic Carcinoma Stem Cell Differentiation. J Neurosci 10 e0143255 PMID: 26571119
Chantelle F et al (2020) FUS contributes to mTOR-dependent inhibition of translation. J Biol Chem 295 18459-18473 PMID: 33453847
Chantelle F et al (2020) FUS contributes to mTOR-dependent inhibition of translation. J Biol Chem 295 18459-18473 PMID: 33082139
Mark L et al (2020) Local miRNA-Dependent Translational Control of GABAAR Synthesis during Inhibitory Long-Term Potentiation. Cell Rep 31 107785 PMID: 32579917
Philipp et al (2020) An Anti-PSMA Immunotoxin Reduces Mcl-1 and Bcl2A1 and Specifically Induces in Combination with the BAD-Like BH3 Mimetic ABT-737 Apoptosis in Prostate Cancer Cells. Cancers (Basel) 12 PMID: 32580291
Leung et al (2016) WNT/β-catenin signaling mediates human neural crest induction via a pre-neural border intermediate. Development 143 398 PMID: 26839343
Luke A et al (2016) Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation. Elife 5 PMID: 27661255
Dasgupta et al (2015) Reduced Levels of Proteasome Products in a Mouse Striatal Cell Model of Huntington's Disease. PLoS One 10 e0145333 PMID: 26691307
Tao et al (2021) CT45A1 promotes the metastasis of osteosarcoma cells in vitro and in vivo through β-catenin. Cell Death Dis 12 650 PMID: 34172717
Balaji M et al (2021) Two distinct trophectoderm lineage stem cells from human pluripotent stem cells. J Biol Chem 296 100386 PMID: 33556374
Nabil G et al (2021) PCSK9 regulates the NODAL signaling pathway and cellular proliferation in hiPSCs. Stem Cell Reports 16 2958-2972 PMID: 34739847
Yang et al (2019) SIGMAR1/Sigma-1 receptor ablation impairs autophagosome clearance. Autophagy 1 PMID: 30871407
Yu et al (2019) Synergistic antitumor effects of 9.2.27-PE38KDEL and ABT-737 in primary and metastatic brain tumors. PLoS One 14 e0210608 PMID: 30625226
Ma et al (2017) AGR2 promotes the proliferation, migration and regulates epithelial-mesenchymal transition in salivary adenoid cystic carcinoma. Am J Transl Res. 9 507 PMID: 28337279
Do you know of a great paper that uses Puromycin dihydrochloride from Tocris? Please let us know.
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Puromycin maintains stable expression of recombinant hRyR2 in HEK293 cells..
By
Niroj Shrestha on 01/20/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293
Puromycin (5 mcg/ml) along with geneticin (250 mcg/ml) was used in DMEM containing 10% FBS, 4 mM glutamine and 10 mM HEPES to maintain the stable expression of hRyR2-IRES-GFP in HEK293 cell culture. The stable expression of hRyR2 in these cells was confirmed by cytosolic GFP fluorescence and 10 mM caffeine-evoked cytosolic Ca2+ peak, measured by Fura2 ratiometric dye, as shown in figure.
Puromycin should be stored in aliquots at -20 degree C.
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