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Submit ReviewDoxycycline hyclate
Description: Broad-spectrum MMP inhibitor; tetracycline derivative
Chemical Name: (4S,4aR,5S,5aR,6R,12aS)-4-(Dimethylamino)- 3,5,10,12,12a-pentahydroxy- 6-methyl- 1,11-dioxo- 1,4,4a,5,5a,6,11,12a-octahydrotetracene- 2-carboxamide hydrochloride hemiethanolate hemihydrate
Purity: ≥92% (HPLC)
Biological Activity for Doxycycline hyclate
Doxycycline hyclate is a broad-spectrum antibiotic and MMP inhibitor; tetracycline derivative. Doxycycline hyclate increases and promotes smooth muscle cell adhesion; inhibits proliferation and migration. Protects the microvasculature by inhibiting plasminogen systems.
For more information about how Doxycycline hyclate may be used, see our protocols: Transdifferentiating Fibroblasts into Neurons, Reprogramming MEFs into Skeletal Muscle Progenitors.
Technical Data for Doxycycline hyclate
| M. Wt | 512.94 |
| Formula | C22H24N2O8.HCl.0.5C2H6O.0.5H2O |
| Storage | Store at +4°C |
| Purity | ≥92% (HPLC) |
| CAS Number | 24390-14-5 |
| PubChem ID | 54686183 |
| InChI Key | UHHHTIKWXBRCLT-VDBOFHIQSA-N |
| Smiles | OC1=C(C(C2=C(O)[C@@](C(C(C(N)=O)=C(O)[C@H]4N(C)C)=O)(O)[C@@]4([H])[C@@H](O)[C@@]([H])2[C@H]3C)=O)C3=CC=C1.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Doxycycline hyclate
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 48.09 | 100 | |
| DMSO | 48.09 | 100 |
Preparing Stock Solutions for Doxycycline hyclate
The following data is based on the product molecular weight 512.94. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.95 mL | 9.75 mL | 19.5 mL |
| 5 mM | 0.39 mL | 1.95 mL | 3.9 mL |
| 10 mM | 0.19 mL | 0.97 mL | 1.95 mL |
| 50 mM | 0.04 mL | 0.19 mL | 0.39 mL |
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Product Datasheets for Doxycycline hyclate
References for Doxycycline hyclate
References are publications that support the biological activity of the product.
Franco et al (2006) Doxycycline alters vascular smooth muscle cell adhesion, migration, and reorganization of fibrillar collagen matrices. Am.J.Pathol. 168 1697 PMID: 16651635
Burggraf et al (2007) Doxycycline inhibits MMPs via modulation of plasminogen activators in focal cerebral ischemia. Neurobiol.Dis. 25 506 PMID: 17166729
Wernig et al (2008) A drug-inducible transgenic system for direct reprogramming of multiple somatic cell types. Nat.Biotechnol. 26 916 PMID: 18594521
If you know of a relevant reference for Doxycycline hyclate, please let us know.
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Keywords: Doxycycline hyclate, Doxycycline hyclate supplier, tetracycline, antibiotics, inhibits, MMPs, matrix, metalloprotease, metalloproteinase, inhibitors, Matrix, Metalloprotease, Antibiotics, 4090, Tocris Bioscience
⚠ WARNING: This product can expose you to chemicals including Doxycycline hyclate (internal use), which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov
9 Citations for Doxycycline hyclate
Citations are publications that use Tocris products. Selected citations for Doxycycline hyclate include:
Ruchira et al (2021) 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration. Cell Stem Cell 28 846-862.e8 PMID: 33784497
Enrique et al (2021) Chromosome compartments on the inactive X guide TAD formation independently of transcription during X-reactivation. Nat Commun 12 3499 PMID: 34108480
Balaji M et al (2021) Two distinct trophectoderm lineage stem cells from human pluripotent stem cells. J Biol Chem 296 100386 PMID: 33556374
Ling et al (2021) Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids. Cell Stem Cell 28 1090-1104.e6 PMID: 33915081
Sato et al (2019) Core Transcription Factors Promote Induction of PAX3-Positive Skeletal Muscle Stem Cells. Stem Cell Reports 13 352 PMID: 31353225
Connor H et al (2022) Dynamic spreading of chromatin-mediated gene silencing and reactivation between neighboring genes in single cells. Elife 11 PMID: 35678392
Mitinori et al (2022) Controlled X-chromosome dynamics defines meiotic potential of female mouse in vitro germ cells. EMBO J 41 e109457 PMID: 35603814
Kalous et al (2012) Neurotrophic actions initiated by proNGF in adult sensory neurons may require peri-somatic glia to drive local cleavage to NGF. J Neurochem 122 523 PMID: 22621370
Rukhsana et al (2020) An Update of the Virion Proteome of Kaposi Sarcoma-Associated Herpesvirus. Viruses 12 PMID: 33276600
Do you know of a great paper that uses Doxycycline hyclate from Tocris? Please let us know.
Reviews for Doxycycline hyclate
Average Rating: 4 (Based on 1 Review.)
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Doxycycline was used to study its resistance pattern against Pseudomonas aeruginosa.
By
Anonymous on 02/09/2019
Species: Human
Doxycycline was used to study its resistance pattern against Pseudomonas aeruginosa. E coli was used as a standard to compare.
Protocols for Doxycycline hyclate
The following protocols feature additional information for the use of Doxycycline hyclate (Cat. No. 4090).
Literature in this Area
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