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Cyclophosphamide

Cat. No. 4091 Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: Alkylating agent; chemotherapeutic
Chemical Name: 2-[Bis(2-chloroethyl)amino]tetrahydro-2H-1,3,2-oxazaphosphorine 2-oxide
Purity: ≥99% (HPLC)
Datasheet
Citations
Reviews
Literature (1)
Pathways (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for Cyclophosphamide

Cyclophosphamide is a nitrogen mustard alkylating agent and prodrug. Phosphoramide mustard (active metabolite) forms DNA cross-links leading to cell death. Inhibits aldehyde dehydrogenase 1 (ALDH1) through its degradation product acrolein. Chemotherapeutic for the treatment of breast cancer; regulates Bax and Bcl-2 expression when administered with etoposide (Cat. No. 1226) in breast cancer cell lines.

Technical Data for Cyclophosphamide

M. Wt 261.09
Formula C7H15Cl2N2O2P
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 50-18-0
PubChem ID 2907
InChI Key CMSMOCZEIVJLDB-UHFFFAOYSA-N
Smiles O=P1(N(CCCl)CCCl)OCCCN1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for Cyclophosphamide

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 26.11 100
DMSO 26.11 100

Preparing Stock Solutions for Cyclophosphamide

The following data is based on the product molecular weight 261.09. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.83 mL 19.15 mL 38.3 mL
5 mM 0.77 mL 3.83 mL 7.66 mL
10 mM 0.38 mL 1.92 mL 3.83 mL
50 mM 0.08 mL 0.38 mL 0.77 mL

Molarity Calculator

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Product Datasheets for Cyclophosphamide

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for Cyclophosphamide

References are publications that support the biological activity of the product.

Gibson et al (1999) Regulation of BAX and BCL-2 expression in breast cancer cells by chemotherapy. Breast Cancer Research and Treatment 55 107 PMID: 10481938

Hengstler et al (1992) DNA strand breaks and DNA cross-links in peripheral mononuclear blood cells of ovarian cancer patients during chemotherapy with cyclophosphamide/carboplatin. Cancer Res. 15 52

Ren et al (1999) Inhibition of human aldehyde dehydrogenase 1 by the 4-hydroxycyclophosphamide degradation product acrolein. Drug Metab.Disp. 27 133

If you know of a relevant reference for Cyclophosphamide, please let us know.

View Related Products by Target

Keywords: Cyclophosphamide, Cyclophosphamide supplier, chemotherapeutics, cross-links, alkylates, DNA, cytotoxic, inhibits, inhibitors, aldehyde, dehydrogenase, 1, ALDH1, Apoptosis, Inducers, DNA,, RNA, and, Protein, Synthesis, 4091, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Cyclophosphamide (anhydrous), which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov

Citations for Cyclophosphamide

Citations are publications that use Tocris products.

Currently there are no citations for Cyclophosphamide. Do you know of a great paper that uses Cyclophosphamide from Tocris? Please let us know.

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

Programmed Cell Death Poster

Programmed Cell Death Poster

There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.

Pathways for Cyclophosphamide

Apoptosis Signaling Pathway

Apoptosis Signaling Pathway

Apoptosis is a physiological process for cell death that is critical during aging and development. It may also be referred to as cell 'suicide'. Apoptosis can be triggered by events both inside and outside of the cell.