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Submit ReviewPoly(I:C)
Description: TLR3 agonist
Chemical Name: Polyinosinic-polycytidylic acid sodium salt
Biological Activity for Poly(I:C)
Poly(I:C) is a synthetic double-stranded RNA (dsRNA); endosomal Toll-like receptor 3 (TLR3) agonist. It also binds to the cytoplasmic receptors, retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated gene 5 (MDA-5), elicits the secretion of type I interferon (IFN) and pro-inflammatory cytokines. Poly(I:C) is commonly used for immune system activation in vivo. Poly(I:C) also promotes maturation of hPSC-derived cardiomyocytes by modulating early Notch signaling and cardiomyogenic transcriptional programs. Poly(I:C) promotes generation of stable, mature dendritic cells in combination with TLR8 agonist CL 075 (Cat. No. 6142). Transfection into NIT-1 β cells induces β-cell apoptosis.
To prevent denaturation, this product should be reconstituted in solutions with physiological salt concentrations.
Technical Data for Poly(I:C)
| Formula | (C10H13N4O8P)x.(C9H14N3O8P)x |
| Storage | Store at -20°C |
| CAS Number | 42424-50-0 |
| PubChem ID | 32744 |
| InChI Key | ACEVNMQDUCOKHT-ZLOOHWKQSA-N |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Poly(I:C)
| Solubility | Soluble to 3 mg/ml in water |
Product Datasheets for Poly(I:C)
References for Poly(I:C)
References are publications that support the biological activity of the product.
Robbins et al (2003) Nuclear factor-κB translocation mediates double-stranded ribonucleic acid-induced NIT-1 beta-cell apoptosis and up-regulates caspase-12 and tumor necrosis factor-associated ligand (TRAIL). Endocrinology 144 4616 PMID: 12960048
Rouas et al (2004) Poly(I:C) used for human dendritic cell maturation preserves their ability to secondarily secrete bioactive IL-12. Int.Immunol. 16 767 PMID: 15096480
West et al (2011) TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature 472 476 PMID: 21525932
Spranger et al (2010) Generation of Th1-polarizing dendritic cells using the TLR7/8 agonist CL075. J.Immunol. 185 738 PMID: 20511554
Biermann et al (2019) Epigenetic priming of human pluripotent stem cell-derived cardiac progenitor cells accelerates cardiomyocyte maturation. Stem Cells 37 910 PMID: 31087611
Waele et al (2021) A systematic review on poly(I:C) and poly-ICLC in glioblastoma: adjuvants coordinating the unlocking of immunotherapy. J.Exp.Clin.Cancer Res. 40 213 PMID: 34172082
If you know of a relevant reference for Poly(I:C), please let us know.
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Keywords: Poly(I:C), Poly(I:C) supplier, Poly(I:C), immunostimulant, toll-like, receptor, TLR, TLR3, agonist, inosinic, acid, cytidylic, double-stranded, RNA, dsRNA, synthetic, maternal, immune, activation, MIA, cardiomyocyte, differentiation, maturation, RIG-I, MDA-5, IFN, Notch, signaling, Toll-like, Receptors, Cardiomyocyte, Stem, Cells, 4287, Tocris Bioscience
20 Citations for Poly(I:C)
Citations are publications that use Tocris products. Selected citations for Poly(I:C) include:
Tachibana et al (2022) Poly I:C and R848 facilitate nitric oxide production via inducible nitric oxide synthase in chicks. Comp.Biochem.Physiol.A Mol.Integr.Physiol. 269 111211 PMID: 35417747
Mingjun et al (2023) A p53-TLR3 axis ameliorates pulmonary hypertension by inducing BMPR2 via IRF3. iScience 26 105935 PMID: 36685041
Alyssa H et al (2023) Weight cycling induces innate immune memory in adipose tissue macrophages. Front Immunol 13 984859 PMID: 36713396
Anne et al (2023) Genetic adaptation to pathogens and increased risk of inflammatory disorders in post-Neolithic Europe. Cell Genom 3 100248 PMID: 36819665
Alexa et al (2023) Anti-inflammatory properties of antiangiogenic fucoidan in retinal pigment epithelium cells. Heliyon 9 e15202 PMID: 37123974
Hideaki et al (2020) TRIM21 Dysfunction Enhances Aberrant B-Cell Differentiation in Autoimmune Pathogenesis. Front Immunol 11 98 PMID: 32117252
Yu et al (2018) Cryo-EM Structures of MDA5-dsRNA Filaments at Different Stages of ATP Hydrolysis. Mol Cell 72 999 PMID: 30449722
Shahram et al (2021) EZH2 Inhibition Compromises α4-1BB-Mediated Antitumor Efficacy by Reducing the Survival and Effector Programming of CD8+ T Cells. Front Immunol 12 770080 PMID: 34925340
Ivan et al (2021) PERK Is Critical for Alphavirus Nonstructural Protein Translation. Viruses 13 PMID: 34065980
Yi et al (2021) ADAM17 Boosts Cholesterol Efflux and Downstream Effects of High-Density Lipoprotein on Inflammatory Pathways in Macrophages. Arterioscler Thromb Vasc Biol 41 1854-1873 PMID: 33882688
Veronica Elizabeth et al (2021) Virus Infection Induces Keap1 Binding to Cytokine Genes, Which Recruits NF-κB p50 and G9a-GLP and Represses Cytokine Transcription. J Immunol 207 1437-1447 PMID: 34400522
Yue et al (2018) PDGFRβ Cells Rapidly Relay Inflammatory Signal from the Circulatory System to Neurons via Chemokine CCL2. Neuron 100 183-200.e8 PMID: 30269986
Masayuki et al (2016) Systemic Activation of TLR3-Dependent TRIF Signaling Confers Host Defense against Gram-Negative Bacteria in the Intestine. Front Cell Infect Microbiol 5 105 PMID: 26793623
Nicolas et al (2019) IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence. Brain Behav Immun 79 274-283 PMID: 30776474
Tröscher et al (2019) Microglial nodules provide the environment for pathogenic T cells in human encephalitis. Acta Neuropathol 137 619 PMID: 30663001
Scott W et al (2022) TRAF6 functions as a tumor suppressor in myeloid malignancies by directly targeting MYC oncogenic activity. Cell Stem Cell 29 298-314.e9 PMID: 35045331
Xuefei et al (2022) Stem cell-derived porcine macrophages as a new platform for studying host-pathogen interactions. BMC Biol 20 14 PMID: 35027054
Takashi et al (2022) Human Lung Fibroblasts Exhibit Induced Inflammation Memory via Increased IL6 Gene Expression and Release. Front Immunol 13 921728 PMID: 35941890
Sandra et al (2022) Assessing human iPSC-derived microglia identity and function by immunostaining, phagocytosis, calcium activity, and inflammation assay. STAR Protoc 3 101866 PMID: 36595902
Johann et al (2022) Pro-inflammatory activation changes intracellular transport of bevacizumab in the retinal pigment epithelium in vitro. Graefes Arch Clin Exp Ophthalmol 260 857-872 PMID: 34643794
Do you know of a great paper that uses Poly(I:C) from Tocris? Please let us know.
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Poly IC.
By
shubham dubey on 12/22/2021
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: 16HBE
To activate TLR3
Pathways for Poly(I:C)
