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T2AA

Cat. No. 4723 1 Citation 1 Review Submit a Review Datasheet / COA / SDS
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Description: PCNA inhibitor
Chemical Name: (βS)-β-Amino-4-(4-hydroxyphenoxy)-3,5-diiodobenzenpropanol
Purity: ≥98% (HPLC)
Datasheet
Citations (1)
Reviews (1)
Literature (2)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for T2AA

T2AA is a proliferating cell nuclear antigen (PCNA) inhibitor; inhibits PCNA/PIP-box peptide interaction (IC50 ~1 μM). Causes DNA replication stress by stalling DNA replication forks. Inhibits PCNA interaction with DNA polymerase δ and arrests cell growth in S-phase. Suppresses cancer cell growth.

Licensing Information

Sold under license from St. Jude Childrens Research Hospital

Technical Data for T2AA

M. Wt 511.09
Formula C15H15I2NO3
Storage Store at -20°C
Purity ≥98% (HPLC)
CAS Number 1380782-27-3
PubChem ID 71185540
InChI Key NKOSKXJRVWVXRI-JTQLQIEISA-N
Smiles IC1=C(OC2=CC=C(O)C=C2)C(I)=CC(C[C@H](N)CO)=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for T2AA

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 51.11 100

Preparing Stock Solutions for T2AA

The following data is based on the product molecular weight 511.09. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.96 mL 9.78 mL 19.57 mL
5 mM 0.39 mL 1.96 mL 3.91 mL
10 mM 0.2 mL 0.98 mL 1.96 mL
50 mM 0.04 mL 0.2 mL 0.39 mL

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References for T2AA

References are publications that support the biological activity of the product.

Punchihewa et al (2012) Identification of small molecule proliferating cell nuclear antigen (PCNA) inhibitor that disrupts interactions with PIP-box proteins and inhibits DNA replication. J.Biol.Chem. 287 14289 PMID: 22383522

If you know of a relevant reference for T2AA, please let us know.

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Keywords: T2AA, T2AA supplier, Proliferating, cell, nuclear, antigen, PCNA, inhibitors, inhibits, DNA, replication, forks, DNA,, RNA, and, Protein, Synthesis, Polymerase, 4723, Tocris Bioscience

1 Citation for T2AA

Citations are publications that use Tocris products. Selected citations for T2AA include:

Carl L et al (2020) Dynamic Assembly and Disassembly of the Human DNA Polymerase δ Holoenzyme on the Genome In Vivo. Cell Rep 30 1329-1341.e5 PMID: 32023453

Do you know of a great paper that uses T2AA from Tocris? Please let us know.

Reviews for T2AA

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T2AA completely kills melanoma cell line at micromolar concentrations.
By Anonymous on 02/07/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: SK-MEL-28

Used for killing assay for in vitro cell culture. Cells were cultured for 24 hours before the addition of the compound into the media at low micromolar concentrations (indicated by the arrow).

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Literature in this Area

Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!

*Please note that Tocris will only send literature to established scientific business / institute addresses.

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