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Description: Selective inhibitor of TGF-β signaling; induces cardiomyocyte differentiation in ESCs
Chemical Name: 4-[1,1'-Biphenyl]-4-yl-1,4,5,6,7,8-hexahydro-2,7,7-trimethyl-5-oxo-3-quinolinecarboxylic acid ethyl ester
Purity: ≥98% (HPLC)
Biological Activity for ITD 1
ITD 1 is a selective inhibitor of TGF-β signaling (IC50 = 0.85 μM); displays little or no inhibition of activin, Wnt or BMP signaling pathways. Induces proteasomal degradation of the TGF-β type II receptor. Inhibits TGF-β-induced mesoderm formation from mouse embryonic stem cells (ESCs) during early differentiation; selectively promotes the differentiation of ESCs to cardiomyocytes in vitro between days 3-5. Does not induce differentiation of vascular smooth muscle cells or endothelial cells.
Compound Libraries for ITD 1
ITD 1 is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
Technical Data for ITD 1
| M. Wt | 415.52 |
| Formula | C27H29NO3 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1099644-42-4 |
| PubChem ID | 44135961 |
| InChI Key | ULFUJLFTRWWLPO-UHFFFAOYSA-N |
| Smiles | CC(C3)(C)CC(C1=C3NC(C)=C(C(OCC)=O)C1C2=CC=C(C4=CC=CC=C4)C=C2)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for ITD 1
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 8.31 | 20 | |
| ethanol | 4.16 | 10 with gentle warming |
Preparing Stock Solutions for ITD 1
The following data is based on the product molecular weight 415.52. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 12.03 mL | 60.17 mL | 120.33 mL |
| 1 mM | 2.41 mL | 12.03 mL | 24.07 mL |
| 2 mM | 1.2 mL | 6.02 mL | 12.03 mL |
| 10 mM | 0.24 mL | 1.2 mL | 2.41 mL |
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Product Datasheets for ITD 1
References for ITD 1
References are publications that support the biological activity of the product.
Willems et al (2012) Small molecule-mediated TGF-beta type II receptor degradation promotes cardiomyogenesis in embryonic stem cells. Cell Stem Cell 11 242 PMID: 22862949
Kinney et al (2013) Emerging strategies for spatiotemporal control of stem cell fate and morphogenesis. Trends Biotechnol. 31 78 PMID: 23219200
If you know of a relevant reference for ITD 1, please let us know.
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4 Citations for ITD 1
Citations are publications that use Tocris products. Selected citations for ITD 1 include:
Andrew R et al (2021) Runt related transcription factor-1 plays a central role in vessel co-option of colorectal cancer liver metastases. Commun Biol 4 950 PMID: 34376784
Chen et al (2021) Landscape of innate lymphoid cells in human head and neck cancer reveals divergent NK cell states in the tumor microenvironment. Proc Natl Acad Sci U S A 118 PMID: 34244432
Smyth (2018) TGF-β mimic proteins form an extended gene family in the murine parasite Heligmosomoides polygyrus. Int J Parasitol 48 379 PMID: 29510118
Yi-Wen et al (2020) TGF-β-driven muscle degeneration and failed regeneration underlie disease onset in a DMD mouse model. JCI Insight 5 PMID: 32213706
Do you know of a great paper that uses ITD 1 from Tocris? Please let us know.
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Literature in this Area
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Stem Cell Research Product Guide
This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:
- Self-renewal and Maintenance
- Differentiation
- Reprogramming
- Organoid Generation
- GMP and Ancillary Material Grade Products
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.
Pathways for ITD 1
