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ML 297

Cat. No. 5380 1 Review Submit a Review Datasheet / COA / SDS
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Description: Selective Kir3.1/3.2 (GIRK1/2) channel activator
Chemical Name: N-(3,4-Difluorophenyl)-N'-(3-methyl-1-phenyl-1H-pyrazol-5-yl)urea
Purity: ≥99% (HPLC)
Datasheet
Citations
Reviews (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for ML 297

ML 297 is a selective Kir3.1/3.2 (GIRK1/2) channel activator (IC50 values are 160, 887 and 914 nM for GIRK1/2, GIRK1/4 and GIRK1/3 respectively). Exhibits no effect on GIRK2, GIRK2/3, Kir2.1 and Kv7.4 channels, and has minimal effect on a panel of other ion channels, receptors and transporters. Prevents PTZ-induced epileptic seizures in mice. Brain penetrant.

Licensing Information

Sold under license from Vanderbilt University

Compound Libraries for ML 297

ML 297 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.

Technical Data for ML 297

M. Wt 328.32
Formula C17H14F2N4O
Storage Store at -20°C
Purity ≥99% (HPLC)
CAS Number 1443246-62-5
PubChem ID 56642816
InChI Key IEKSMUSSYJUQMY-UHFFFAOYSA-N
Smiles O=C(NC2=CC(C)=NN2C3=CC=CC=C3)NC1=CC(F)=C(F)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for ML 297

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 32.83 100
ethanol 16.42 50

Preparing Stock Solutions for ML 297

The following data is based on the product molecular weight 328.32. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.05 mL 15.23 mL 30.46 mL
5 mM 0.61 mL 3.05 mL 6.09 mL
10 mM 0.3 mL 1.52 mL 3.05 mL
50 mM 0.06 mL 0.3 mL 0.61 mL

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References for ML 297

References are publications that support the biological activity of the product.

Kaufmann et al (2013) ML297 (VU0456810), the first potent and selective activator of the GIRK potassium channel, displays antiepileptic properties in mice. ACS Chem.Neurosci. 4 1278 PMID: 23730969

Wen et al (2013) Discovery of 'molecular switches' within a GIRK activator scaffold that afford selective GIRK inhibitors. Bioorg.Med.Chem.Lett. 23 4562 PMID: 23838260

If you know of a relevant reference for ML 297, please let us know.

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Citations for ML 297

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Reviews for ML 297

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ML 297 worked ideally and the data obtained with the product was consistent throughout the study.
By Anonymous on 10/31/2018
Assay Type: In Vitro
Species: Human

ML 297 and ML 277 was used on our University labs to understand potassium channel activation percentage at increasing concentrations. ML 297 worked ideally and the data obtained with the product was consistent throughout the experiment.

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