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Description: Calcium chelating agent
Chemical Name: Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid
Biological Activity for EGTA
EGTA is a calcium chelator; protects against cell death caused by nitric oxide-induced calcium influx into nerve cells.
Technical Data for EGTA
| M. Wt | 380.35 |
| Formula | C14H24N2O10 |
| Storage | Store at RT |
| CAS Number | 67-42-5 |
| PubChem ID | 6207 |
| InChI Key | DEFVIWRASFVYLL-UHFFFAOYSA-N |
| Smiles | O=C(CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O)O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for EGTA
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| 1M NaOH | 38.03 | 100 |
Preparing Stock Solutions for EGTA
The following data is based on the product molecular weight 380.35. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.63 mL | 13.15 mL | 26.29 mL |
| 5 mM | 0.53 mL | 2.63 mL | 5.26 mL |
| 10 mM | 0.26 mL | 1.31 mL | 2.63 mL |
| 50 mM | 0.05 mL | 0.26 mL | 0.53 mL |
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Product Datasheets for EGTA
References for EGTA
References are publications that support the biological activity of the product.
Boullerne et al (2001) Role of calcium in nitric oxide-induced cytotoxicity: EGTA protects mouse oligodendrocytes. J.Neurosci.Res. 63 124 PMID: 11169622
Fisher et al (2004) Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG. Biochem.Biophy.Res.Comms. 323 163
If you know of a relevant reference for EGTA, please let us know.
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Keywords: EGTA, EGTA supplier, Calcium, chelating, agent, Reagents, Signaling, Signalling, Agents, General, Ca2+, Biochemicals, and, Molecular, Biology, 2807, Tocris Bioscience
6 Citations for EGTA
Citations are publications that use Tocris products. Selected citations for EGTA include:
Chang et al (2013) Areca nut-induced buccal mucosa fibroblast contraction and its signaling: a potential role in oral submucous fibrosis--a precancer condition. PLoS One 34 1096 PMID: 23349021
Zhang et al (2011) Genistein and tyrphostin AG556 inhibit inwardly-rectifying Kir2.1 channels expressed in HEK 293 cells via protein tyrosine kinase inhibition. Biochim Biophys Acta 1808 1993 PMID: 21570948
Gouet et al (2012) On the mechanism of synaptic depression induced by CaMKIIN, an endogenous inhibitor of CaMKII. PLoS One 7 e49293 PMID: 23145145
Lerner et al (2010) Endocannabinoid signaling mediates psychomotor activation by adenosine A2A antagonists. J Neurosci 30 2160 PMID: 20147543
Shambharkar et al (2015) TMEM203 Is a Novel Regulator of Intracellular Calcium Homeostasis and Is Required for Spermatogenesis. BMC Complement Altern Med 10 e0127480 PMID: 25996873
Ahn et al (2015) Neuroprotection and spatial memory enhancement of four herbal mixture extract in HT22 hippocampal cells and a mouse model of focal cerebral ischemia. Carcinogenesis 15 202 PMID: 26122524
Do you know of a great paper that uses EGTA from Tocris? Please let us know.
Reviews for EGTA
Average Rating: 4.5 (Based on 2 Reviews.)
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EGTA activates CRAC current via passive ER Ca2+ depletion in HEK293 cells co-expressing YFP-STIM1 and Orai1-CFP..
By
Anonymous on 12/16/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HEK293
ER Ca2+ in HEK293 cells co-expressing YFP-STIM1 and Orai1-CFP was passively depleted with pipette solution, containing (in mM): 20 EGTA, 145 Cs-methanesulfonate, 8 NaCl, 5 MgCl2 and 10 HEPES, pH 7.2 with KOH.
EGTA inhibits 15d-PGJ2 induced TNFa production by cardiomyocytes.
By
Anonymous on 12/10/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: cardiomyocytes
HL-1 cells were incubated with EGTA (5 mM) for 30 min prior to 15d-PGJ2 (15 µM) treatment for 4 h to follow TNFa concentrations in the cell culture medium. Addition of EGTA resulted in abolition of 15d-PGJ2 induced TNFa production.
