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Submit ReviewWithaferin A
Description: Inhibits NF-κB activation
Chemical Name: (4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone
Purity: ≥98% (HPLC)
Biological Activity for Withaferin A
Withaferin A is a steroid lactone that displays anti-inflammatory, antitumor and antiangiogenic activity. Withaferin A inhibits endothelial cells (HUVEC) sprouting in vitro (IC50 = 12 nM) and in vivo, prevents NF-κB activation by inhibiting activation of IKKβ and inhibits chymotrypsin-like activity of the 20S proteasome. In an animal model of Huntington's disease (HD), Withaferin A delays disease progression and increases lifespan; in a cellular model it suppresses aggregation of mutant huntingtin, activates HSF1 and induces Hsp70 chaperone expression.
Technical Data for Withaferin A
| M. Wt | 470.6 |
| Formula | C28H38O6 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 5119-48-2 |
| PubChem ID | 45489105 |
| InChI Key | DBRXOUCRJQVYJQ-WDBPBROTSA-N |
| Smiles | O=C1[C@@]2(C)[C@@]([C@H]5C[C@]3([H])C2CC[C@@]4(C)[C@]([H])3CC[C@@H]4[C@@H]([C@]6([H])OC(C(CO)=C(C)C6)=O)C)(O5)[C@@H](O)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Withaferin A
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.71 | 10 |
Preparing Stock Solutions for Withaferin A
The following data is based on the product molecular weight 470.6. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 21.25 mL | 106.25 mL | 212.49 mL |
| 0.5 mM | 4.25 mL | 21.25 mL | 42.5 mL |
| 1 mM | 2.12 mL | 10.62 mL | 21.25 mL |
| 5 mM | 0.42 mL | 2.12 mL | 4.25 mL |
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Product Datasheets for Withaferin A
References for Withaferin A
References are publications that support the biological activity of the product.
Mohan et al (2004) Withaferin A is a potent inhibitor of angiogenesis. Angiogenesis 7 115 PMID: 15516832
Yang et al (2007) The tumor proteasome is a primary target for the natural anticancer compound withaferin A isolated from "Indian Winter Cherry". Mol.Pharmacol. 71 426 PMID: 17093135
Kaileh et al (2007) Withaferin A strongly elicits IkB kinase β hyperphosphorylation concomitant with potent inhibition of its kinase activity. J.Biol.Chem. 282 4253 PMID: 17150968
Joshi et al (2021) Withaferin A induces heat shock response and ameliorates disease progression in a mouse model of Huntington's disease. Mol.Neurobiol. 58 3992 PMID: 33904021
If you know of a relevant reference for Withaferin A, please let us know.
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Keywords: Withaferin A, Withaferin A supplier, inhibitors, inhibits, NF-κB, NF-kappaB, NF-kB, activation, Nuclear, Factor, Kappa, B, Cytokine, Signaling, Signalling, Transcription, Factors, WithaferinA, NF-kB/IkB, 2816, Tocris Bioscience
7 Citations for Withaferin A
Citations are publications that use Tocris products. Selected citations for Withaferin A include:
Andersson et al (2018) Molecular mechanisms of IL-33-mediated stromal interactions in cancer metastasis. JCI Insight 3 PMID: 30333314
Ryals et al (2018) The Inner Ear Heat Shock Transcriptional Signature Identifies Compounds That Protect Against Aminoglycoside Ototoxicity. Front Cell Neurosci 12 445 PMID: 30532693
Hatzold et al (2016) Tumor suppression in basal keratinocytes via dual non-cell-autonomous functions of a Na,K-ATPase beta subunit. Elife 5 PMID: 27240166
Yang et al (2016) The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages. Neural Plast 7 11385 PMID: 27150562
Martorana et al (2015) Withaferin A Inhibits Nuclear Factor-κB-Dependent Pro-Inflammatory and Stress Response Pathways in the Astrocytes. EMBO J 2015 381964 PMID: 26266054
Trendelenburg et al (2012) TAK-1/p38/nNFκB signaling inhibits myoblast differentiation by increasing levels of AVNA. Skelet Muscle 2 3 PMID: 22313861
Yadav et al (2010) Celastrol suppresses invasion of colon and pancreatic cancer cells through the downregulation of expression of CXCR4 chemokine receptor. J Mol Med (Berl) 88 1243 PMID: 20798912
Do you know of a great paper that uses Withaferin A from Tocris? Please let us know.
Reviews for Withaferin A
Average Rating: 4.5 (Based on 2 Reviews.)
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42 perturbagens used the zebrafish DASPEI screen.
By
Anonymous on 12/17/2019
Assay Type: In Vivo
Species: Other
Cell Line/Tissue: larvae
Zebrafish larvae were placed into 48-well plates and used the DASPEI screen by 42 perturbagens drugs, including this compound
Withaferin inhibits LPS-induced TNFalpha production in macrophages.
By
Christopher Trummer on 10/22/2018
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: Macrophages
RAW macrophages were stimulated with Withaferin (1 µM) alone or in the presence of LPS (200 ng/ml) for 8 h to follow TNFalpha mRNA expression using qPCR. Withaferin significantly inhibited LPS-induced TNFalpha production.
Pathways for Withaferin A
