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Submit ReviewAcetylcholine chloride
Description: Endogenous neurotransmitter
Chemical Name: 2-(Acetyloxy)-N,N,N-trimethylethanaminium chloride
Biological Activity for Acetylcholine chloride
Acetylcholine chloride is an endogenous neurotransmitter. Acts at nicotinic and muscarinic acetylcholine receptors.
Technical Data for Acetylcholine chloride
| M. Wt | 181.66 |
| Formula | C7H16ClNO2 |
| Storage | Desiccate at RT |
| CAS Number | 60-31-1 |
| PubChem ID | 6060 |
| InChI Key | JUGOREOARAHOCO-UHFFFAOYSA-M |
| Smiles | CC(OCC[N+](C)(C)C)=O.[Cl-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Acetylcholine chloride
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 18.17 | 100 |
Preparing Stock Solutions for Acetylcholine chloride
The following data is based on the product molecular weight 181.66. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 5.5 mL | 27.52 mL | 55.05 mL |
| 5 mM | 1.1 mL | 5.5 mL | 11.01 mL |
| 10 mM | 0.55 mL | 2.75 mL | 5.5 mL |
| 50 mM | 0.11 mL | 0.55 mL | 1.1 mL |
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Product Datasheets for Acetylcholine chloride
References for Acetylcholine chloride
References are publications that support the biological activity of the product.
Wessler et al (1999) The cholinergic 'pitfall': acetylcholine, a universal cell molecule in biological systems, including humans. Clin.Exp.Pharmacol.Physiol. 26 198 PMID: 10081614
If you know of a relevant reference for Acetylcholine chloride, please let us know.
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Keywords: Acetylcholine chloride, Acetylcholine chloride supplier, Endogenous, neurotransmitter, Acetylcholine, Nicotinic, Receptors, Non-Selective, Subtypes, nAChR, Muscarinic, (Non-selective), Non-selective, Muscarinics, 2809, Tocris Bioscience
5 Citations for Acetylcholine chloride
Citations are publications that use Tocris products. Selected citations for Acetylcholine chloride include:
Paniello et al (2018) Improved adductor function after canine recurrent laryngeal nerve injury and repair using muscle progenitor cells. Laryngoscope 128 E241 PMID: 29219186
Halum et al (2014) Stem cell-derived tissue-engineered constructs for hemilaryngeal reconstruction. Ann Otol Rhinol Laryngol 123 124 PMID: 24574468
Lee and Kim (2019) Basal Forebrain Cholinergic-induced Activation of Cholecystokinin Inhibitory Neurons in the Basolateral Amygdala. Exp Neurobiol 28 320 PMID: 31308792
Deng et al (2013) Label-free cell phenotypic assessment of the biased agonism and efficacy of agonists at the endogenous muscarinic M3 receptors. J Pharmacol Toxicol Methods 68 323 PMID: 23933114
Fairless et al (2013) Membrane potential measurements of isolated neurons using a voltage-sensitive dye. PLoS One 8 e58260 PMID: 23516458
Do you know of a great paper that uses Acetylcholine chloride from Tocris? Please let us know.
Reviews for Acetylcholine chloride
Average Rating: 5 (Based on 2 Reviews.)
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Acetylcholine chloride induces aortic ring relaxation.
By
Chintan Koyani on 10/22/2018
Assay Type: In Vivo
Species: Mouse
Cell Line/Tissue: Aortic ring
C57BL6 mice were administered with LPS (5 mg/kg) alone or in combination with Empagliflozin (1 µM) for 16 h. Afterwards mice were sacrificed and aortic rings were isolated. Norepinephrine pre-contracted rings were incubated with indicated (in Image) concentrations of Acetylcholine chloride (ACh) to follow endothelial relaxation.
Quantification of acetylcholine in tissue by LC-MS/MS..
By
Olga Malysheva on 01/04/2018
Assay Type: In Vitro
Species: Mouse
We used it as an analytical standard for quantification of acetylcholine in brain tissue.
Even 10mM solution does degrades in the -80C freezer. We keep our stock as 100mM and make new every 6 months.
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Nicotinic ACh Receptors Scientific Review
Updated in 2014, this review by Sue Wonnacott summarizes the diverse structure and function of nicotinic acetylcholine receptors and gives an in-depth review of the ligands available for nAChR research. Compounds available from Tocris are listed.