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Submit ReviewU 18666A
Description: Inhibitor of Hedgehog (Hh) signaling. Also inhibits cholesterol synthesis
Chemical Name: (3β)-3-[2-(Diethylamino)ethoxy]androst-5-en-17-one hydrochloride
Biological Activity for U 18666A
U 18666A is an inhibitor of cholesterol synthesis and cellular transport, and weak inhibitor of hedgehog (Hh) signaling. Reduces serum sterol levels in rats in vivo.
Technical Data for U 18666A
| M. Wt | 424.07 |
| Formula | C25H41NO2.HCl |
| Storage | Desiccate at RT |
| CAS Number | 3039-71-2 |
| PubChem ID | 92722 |
| InChI Key | KXNPVXPOPUZYGB-IOVMHBDKSA-N |
| Smiles | [H][C@@]23CC=C1C[C@@H](OCCN(CC)CC)CC[C@@](C)1[C@]([H])2CC[C@@]4(C)[C@]([H])3CCC4=O.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for U 18666A
References for U 18666A
References are publications that support the biological activity of the product.
Cenedella et al (1980) Concentration-dependent effects of AY-9944 and U18666A on sterol synthesis in brain. Biochem.Pharmacol. 29 2751 PMID: 6159896
Incardona et al (2000) Cyclopamine inhibition of sonic hedgehog signal transduction is not mediated through effects on cholesterol transport. Dev.Biol. 224 440 PMID: 10926779
Liscum and Faust (1989) The intracellular transport of low density lipoprotein-derived cholesterol is inhibited in Chinese hamster ovary cells cultured with 3-β-[2-(diethylamino)ethoxy]androst-5-en-17-one. J.Biol.Chem. 264 11796 PMID: 2745416
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Keywords: U 18666A, U 18666A supplier, inhibitors, hedgehog, Hh, signaling, inhibits, cholesterol, synthesis, Signalling, Hedgehog, U18666A, Signaling, 1638, Tocris Bioscience
Citations for U 18666A
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Reviews for U 18666A
Average Rating: 5 (Based on 1 Review.)
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Cholesterol accumulation increased by U18666A treatment.
By
ASHUTOSH SINGHAL on 01/05/2018
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: HeLa
I used 2.5-10 ug/ml of U18666A to inhibit the cholesterol efflux in HeLa cells and it worked perfectly as I observed in U18666A treated HeLa cells accumulated cholesterol significantly. I used Filipin staining to image and quantitate the cholesterol levels.
I dissolved the U18666A in water and stored at -20C in aliquots.
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