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Submit ReviewW-7 hydrochloride
Description: Calmodulin antagonist. Inhibits myosin light chain kinase
Chemical Name: N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride
Purity: ≥98% (HPLC)
Biological Activity for W-7 hydrochloride
W-7 hydrochloride is a calmodulin antagonist. Inhibits Ca2+-calmodulin-dependent phosphodiesterase (IC50 = 28 μM) and myosin light chain kinase (IC50 = 51 μM). Induces shedding of platelet receptors GPVI, GPV and GPIbα.
Compound Libraries for W-7 hydrochloride
W-7 hydrochloride is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.
Technical Data for W-7 hydrochloride
| M. Wt | 377.33 |
| Formula | C16H21ClN2O2S.HCl |
| Storage | Store at RT |
| Purity | ≥98% (HPLC) |
| CAS Number | 61714-27-0 |
| PubChem ID | 124887 |
| InChI Key | OMMOSRLIFSCDBL-UHFFFAOYSA-N |
| Smiles | Cl.NCCCCCCNS(=O)(=O)C1=CC=CC2=C(Cl)C=CC=C12 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for W-7 hydrochloride
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 1.89 | 5 with gentle warming | |
| DMSO | 37.73 | 100 |
Preparing Stock Solutions for W-7 hydrochloride
The following data is based on the product molecular weight 377.33. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.65 mL | 13.25 mL | 26.5 mL |
| 5 mM | 0.53 mL | 2.65 mL | 5.3 mL |
| 10 mM | 0.27 mL | 1.33 mL | 2.65 mL |
| 50 mM | 0.05 mL | 0.27 mL | 0.53 mL |
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Product Datasheets for W-7 hydrochloride
References for W-7 hydrochloride
References are publications that support the biological activity of the product.
Asano et al (1989) Divergent pharmacological effects of three calmodulin antagonists, N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W 7), chlorprom. and calmidazolium, on isometric tension development and myosin light chain phosphorylation in intact bovine trachea J.Pharmacol.Exp.Ther. 251 764 PMID: 2810127
Hidaka et al (1981) N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, a calmodulin antagonist, inhibits cell proliferation. Proc.Natl.Acad.Sci.U.S.A. 78 4354 PMID: 6945588
Itoh and Hidaka (1984) Direct interaction of calmodulin antagonists with Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase. J.Biochem. 96 1721 PMID: 6099352
Tanaka and Hidaka (1980) Hydrophobic regions function in calmodulin-enzyme(s) interactions. J.Biol.Chem. 255 11078 PMID: 6254958
Gardiner et al (2007) Controlled shedding of platelet glycoprotein (GP)VI and GPIb-IX-V by ADAM family metalloproteinases. J.Thromb.Haemost. 5 1530 PMID: 17445093
If you know of a relevant reference for W-7 hydrochloride, please let us know.
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Keywords: W-7 hydrochloride, W-7 hydrochloride supplier, Calmodulin, antagonists, inhibitors, inhibits, myosin, light, chain, kinases, Ca2+, Binding, Protein, modulators, Calcium, Signaling, Signalling, MLCK, Ca2+-calmodulin-dependent, phosphodiesterase, platelet, platelets, Myosin, Light, Chain, Kinase, 0369, Tocris Bioscience
16 Citations for W-7 hydrochloride
Citations are publications that use Tocris products. Selected citations for W-7 hydrochloride include:
Schiff et al (2019) P38 inhibition reverses TGFβ1 and TNFα-induced contraction in a model of proliferative vitreoretinopathy. Commun Biol 2 162 PMID: 31069271
Hou et al (2015) The Actin Nucleator Cobl Is Controlled by Calcium and Calmodulin. Apoptosis 13 e1002233 PMID: 26334624
Bonnefond et al (2015) Calcium signals inhibition sensitizes ovarian carcinoma cells to anti-Bcl-xL strategies through Mcl-1 down-regulation. J Biol Chem 20 535 PMID: 25627260
Araki et al (2015) Rapid dispersion of SynGAP from synaptic spines triggers AMPA receptor insertion and spine enlargement during LTP. J Neurosci 85 173 PMID: 25569349
Gray and Golowasch (2016) Voltage Dependence of a Neuromodulator-Activated Ionic Current. Eneuro 3 PMID: 27257619
Abdou et al (2013) The calcium signaling pathway regulates leydig cell steroidogenesis through a transcriptional cascade involving the nuclear receptor NR4A1 and the steroidogenic acute regulatory protein. Endocrinology 154 511 PMID: 23183170
Haag et al (2018) The Actin Nucleator Cobl Is Critical for Centriolar Positioning, Postnatal Planar Cell Polarity Refinement, and Function of the Cochlea. Cell Rep 24 2418 PMID: 30157434
Lopez et al (2022) Ketamine exerts its sustained antidepressant effects via cell-type-specific regulation of Kcnq2. Neuron 110 2283 PMID: 35649415
Long et al (2009) Nerve Terminal GABAA Receptors Activate Ca2+/Calmodulin-dependent Signaling to Inhibit Voltage-gated Ca2+ Influx and Glutamate Release. Carcinogenesis 284 8726 PMID: 19141616
Yanda et al (2017) Histone deacetylase 6 inhibition reduces cysts by decreasing cAMP and Ca(2+) in knock-out mouse models of polycystic kidney disease. J Biol Chem 292 17897 PMID: 28887310
Yuan et al (2015) Protein kinase G-regulated production of H2S governs oxygen sensing. Sci Signal 8 ra37 PMID: 25900831
Chang et al (2013) Areca nut-induced buccal mucosa fibroblast contraction and its signaling: a potential role in oral submucous fibrosis--a precancer condition. Psychopharmacology (Berl) 34 1096 PMID: 23349021
Mukunda et al (2014) Dimerisation of the Drosophila odorant coreceptor Orco. Neuron 8 261 PMID: 25221476
Midkiff et al (2011) Small molecule inhibitors of the Candida albicans budded-to-hyphal transition act through multiple signaling pathways. PLoS One 6 e25395 PMID: 21966518
Yanes and Romero (2009) Dihydrotestosterone stimulates aldosterone secretion by H295R human adrenocortical cells. Mol Cell Endocrinol 303 50 PMID: 19428991
Dai et al (2011) Phospholipase C, Ca2+, and calmodulin signaling are required for 5-HT2A receptor-mediated transamidation of Rac1 by transglutaminase. Front Cell Neurosci 213 403 PMID: 20717650
Do you know of a great paper that uses W-7 hydrochloride from Tocris? Please let us know.
Reviews for W-7 hydrochloride
Average Rating: 5 (Based on 1 Review.)
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PN and PH cells were treated with W-7 (catalog no. 0369) and W-13 (catalog no. 0361) purchased from Tocris..
By
Anonymous on 11/17/2019
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: PN and PH cells
PN and PH cells were treated with W-7 (catalog no. 0369) and W-13(catalog no. 0361) purchased from Tocris.
