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Submit Review20(S)-Hydroxycholesterol
说明： Allosteric activator of Hedgehog (Hh) signaling; induces Smo accumulation
化学名： (3β)-Cholest-5-ene-3,20-diol
纯度： ≥95% (HPLC)
生物活性 for 20(S)-Hydroxycholesterol
20(S)-Hydroxycholesterol is an allosteric activator of the Hedgehog signaling pathway Smoothened (Smo) oncoprotein; binds at a site distinct from the canonical cyclopamine binding site. Activates Hedgehog (Hh) signaling (EC50 ~ 3μM for induction of Hh reporter gene transcription in in NIH 3T3 cells). Induces Smo accumulation in primary cilia; also exhibits osteogenic activity and activates the liver X receptor (LXR).
技术数据 for 20(S)-Hydroxycholesterol
| 分子量 | 402.65 |
| 公式 | C27H46O2 |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 516-72-3 |
| PubChem ID | 440711 |
| InChI Key | MCKLJFJEQRYRQT-APGJSSKUSA-N |
| Smiles | O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@](O)(C)CCCC(C)C |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for 20(S)-Hydroxycholesterol
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| ethanol | 40.26 | 100 |
制备储备液 for 20(S)-Hydroxycholesterol
以下数据基于产品分子量 402.65。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.48 mL | 12.42 mL | 24.84 mL |
| 5 mM | 0.5 mL | 2.48 mL | 4.97 mL |
| 10 mM | 0.25 mL | 1.24 mL | 2.48 mL |
| 50 mM | 0.05 mL | 0.25 mL | 0.5 mL |
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产品说明书 for 20(S)-Hydroxycholesterol
参考文献 for 20(S)-Hydroxycholesterol
参考文献是支持产品生物活性的出版物。
Nachtergaele et al (2012) Oxysterols are allosteric activators of the oncoprotein Smoothened. Nat.Chem.Biol. 8 211 PMID: 22231273
Dwyer et al (2007) Oxysterols are novel activators of the hedgehog signaling pathway in pluripotent mesenchymal cells J.Biol.Chem. 282 8959 PMID: 17200122
Nedelcu et al (2013) Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling. Nat.Chem.Biol. 9 557 PMID: 23831757
If you know of a relevant reference for 20(S)-Hydroxycholesterol, please let us know.
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关键词: 20(S)-Hydroxycholesterol, 20(S)-Hydroxycholesterol supplier, Oxysterols, allosteric, activators, positive, modulators, Smo, smoothened, Hedgehog, signaling, pathways, Signaling, Stem, Cell, Smoothened, Receptors, 4474, Tocris Bioscience
2 篇 20(S)-Hydroxycholesterol 的引用文献
引用文献是使用了 Tocris 产品的出版物。 20(S)-Hydroxycholesterol 的部分引用包括：
Craig B et al (2017) Metabolic origins of spatial organization in the tumor microenvironment. Proc Natl Acad Sci U S A 114 2934-2939 PMID: 28246332
Lucia et al (2020) HDL inhibits endoplasmic reticulum stress-induced apoptosis of pancreatic β-cells in vitro by activation of Smoothened. J Lipid Res 61 492-504 PMID: 31907205
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