Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit Review8-pCPT-2-O-Me-cAMP-AM
说明： Selective Epac activator; cell-permeable analog of 8CPT-2Me-cAMP (Cat. No. 1645)
别名： 007-AM
化学名： 8-(4-Chlorophenylthio)-2'-O-methyladenosine-3',5'-cyclic monophosphate acetoxymethyl ester
纯度： ≥97% (HPLC)
生物活性 for 8-pCPT-2-O-Me-cAMP-AM
8-pCPT-2-O-Me-cAMP-AM is a selective Epac activator; cAMP analog. Induces Rap activation and junction tightening in HUVECs; triggers adhesion of Jurkat-Epac1 cells to fibronectin. Stimulates insulin secretion in rat INS-1 cells. More potent, cell-permeable analog of 8CPT-2Me-cAMP (Cat. No. 1645).
This product is a mixture of axial and equatorial isomers. Both isomers give 8CPT-2Me-cAMP after esterase cleavage.
许可信息
Sold with the permission of BioLog Life Science Institute.
技术数据 for 8-pCPT-2-O-Me-cAMP-AM
| 分子量 | 557.9 |
| 公式 | C20H21ClN5O8PS |
| 储存 | Store at -20°C |
| 纯度 | ≥97% (HPLC) |
| CAS Number | 1152197-23-3 |
| PubChem ID | 90488978 |
| InChI Key | FZMWUFYPEVDWPA-SILPBKOMSA-N |
| Smiles | NC1=NC=NC2=C1N=C(SC4=CC=C(Cl)C=C4)N2[C@@H]3O[C@@](COP(O5)(OCOC(C)=O)=O)([H])[C@]5([H])[C@H]3OC |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for 8-pCPT-2-O-Me-cAMP-AM
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 55.79 | 100 |
制备储备液 for 8-pCPT-2-O-Me-cAMP-AM
以下数据基于产品分子量 557.9。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.79 mL | 8.96 mL | 17.92 mL |
| 5 mM | 0.36 mL | 1.79 mL | 3.58 mL |
| 10 mM | 0.18 mL | 0.9 mL | 1.79 mL |
| 50 mM | 0.04 mL | 0.18 mL | 0.36 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
产品说明书 for 8-pCPT-2-O-Me-cAMP-AM
参考文献 for 8-pCPT-2-O-Me-cAMP-AM
参考文献是支持产品生物活性的出版物。
Vliem et al (2008) 8-pCPT-2'-O-Me-cAMP-AM: an improved Epac-selective cAMP analogue. Chembiochem. 9 2052 PMID: 18633951
Almahariq et al (2013) A novel EPAC-specific inhibitor suppresses pancreatic cancer cell migration and invasion. Mol.Pharmacol. 83 122 PMID: 23066090
Tsalkova et al (2012) Isoform-specific antagonists of exchange proteins directly activated by cAMP. Proc.Natl.Acad.Sci.U.S.A. 109 18613 PMID: 23091014
Chepurny et al (2009) Enhanced Rap1 activation and Ins secretagogue properties of an acetoxymethyl ester of an Epac-selective cyclic AMP analog in rat INS-1 cells: studies with 8-pCPT-2'-O-Me-cAMP-AM. J.Biol.Chem. 284 10728 PMID: 19244230
If you know of a relevant reference for 8-pCPT-2-O-Me-cAMP-AM, please let us know.
按标靶查看相关产品
按产品操作查看相关产品
关键词: 8-pCPT-2-O-Me-cAMP-AM, 8-pCPT-2-O-Me-cAMP-AM supplier, 007-AM, cAMP, analogs, Epac, 1, 2, epacs, activators, cell, permeable, selective, Rap, guanine, nucleotide, exchange, factor, 3, 4, RAPGEF, GEFI, EPAC, 4853, Tocris Bioscience
10 篇 8-pCPT-2-O-Me-cAMP-AM 的引用文献
引用文献是使用了 Tocris 产品的出版物。 8-pCPT-2-O-Me-cAMP-AM 的部分引用包括：
Diana J et al (2019) Phospho-substrate profiling of Epac-dependent protein kinase C activity. Mol Cell Biochem 456 167-178 PMID: 30739223
Caglar et al (2019) Adenosine Receptor Signaling Targets Both PKA and Epac Pathways to Polarize Dendritic Cells to a Suppressive Phenotype. J Immunol 203 3247-3255 PMID: 31722989
Mishra et al (2018) Reactivation of cAMP Pathway by PDE4D Inhibition Represents a Novel Druggable Axis for Overcoming TAX Resistance in ER-positive Breast Cancer. Clin Cancer Res 24 1987 PMID: 29386221
Jason P et al (2020) Osmotic Gradients in Epithelial Acini Increase Mechanical Tension across E-cadherin, Drive Morphogenesis, and Maintain Homeostasis. Curr Biol 30 624-633.e4 PMID: 31983640
Mayati et al (2018) Functional polarization of human hepatoma HepaRG cells in response to forskolin. Sci Rep 8 1615 PMID: 30382126
Watson et al (2015) Endothelial CD99 signals through soluble adenylyl cyclase and PKA to regulate leukocyte transendothelial migration. Mol Brain 212 1021 PMID: 26101266
Derek C et al (2021) Regulation of Mitochondrial Function by Epac2 Contributes to Acute Inflammatory Hyperalgesia. J Neurosci 41 2883-2898 PMID: 33593853
Itender et al (2018) cAMP regulated EPAC1 supports microvascular density, angiogenic and metastatic properties in a model of triple negative breast cancer. Carcinogenesis 39 1245-1253 PMID: 29982410
Cheng-Mao et al (2018) The EPAC-Rap1 pathway prevents and reverses cytokine-induced retinal vascular permeability. J Biol Chem 293 717-730 PMID: 29158262
Fariba et al (2022) Respiratory syncytial virus disrupts the airway epithelial barrier by decreasing cortactin and destabilizing F-actin. J Cell Sci 135 PMID: 35848790
您是否知道使用了 Tocris 8-pCPT-2-O-Me-cAMP-AM 的优秀论文？ 请告知我们.
8-pCPT-2-O-Me-cAMP-AM 的评论
目前没有该产品的评论。 Be the first to review 8-pCPT-2-O-Me-cAMP-AM and earn rewards!
Have you used 8-pCPT-2-O-Me-cAMP-AM?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image