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Caffeine

Cat. No. 2793 2 篇引用文献 3 条评论 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）
Pricing Availability   Qty
说明： A1 and A2B antagonist. CNS stimulant
化学名： 3,7-Dihydro-1,3,7-trimethyl-1H-purine-2,6-dione
纯度： ≥99% (HPLC)
说明书
引用文献 (2)
评论 (3)
生物活性 技术数据 溶解性 计算器 说明书 参考文献

生物活性 for Caffeine

Caffeine is a central nervous system stimulant. Antagonist at A1 and A2A adenosine receptors and inhibitor of cyclic nucleotide phosphodiesterases. Mobilizes calcium from intracellular stores and inhibits benzodiazepine binding to GABA receptors.

技术数据 for Caffeine

分子量 194.19
公式 C8H10N4O2
储存 Store at RT
纯度 ≥99% (HPLC)
CAS Number 58-08-2
PubChem ID 2519
InChI Key RYYVLZVUVIJVGH-UHFFFAOYSA-N
Smiles O=C1C2=C(N=CN2C)N(C)C(N1C)=O

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Caffeine

溶剂 最高浓度 mg/mL 最高浓度 mM
溶解性
water 19.42 100
DMSO 9.71 50

制备储备液 for Caffeine

以下数据基于产品分子量 194.19。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

选择批次从而根据批次分子量重新计算:
浓度/溶剂体积/质量 1 mg 5 mg 10 mg
1 mM 5.15 mL 25.75 mL 51.5 mL
5 mM 1.03 mL 5.15 mL 10.3 mL
10 mM 0.51 mL 2.57 mL 5.15 mL
50 mM 0.1 mL 0.51 mL 1.03 mL

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参考文献 for Caffeine

参考文献是支持产品生物活性的出版物。

Nehlig et al (1992) Caffeine and the central nervous system: mechanisms of action, biochemical, metabolic and psychostimulant effects. Brain Res.Brain Res.Rev. 17 139 PMID: 1356551

Fisone et al (2004) Caffeine as a psychomotor stimulant: mechanism of action. Cell.Mol.Life Sci. 61 857 PMID: 15095008

Chen et al (2008) Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease. J.Neuroinflammation 5 12 PMID: 18387175

If you know of a relevant reference for Caffeine, please let us know.

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关键词: Caffeine, Caffeine supplier, A1, A2B, antagonists, CNS, stimulant, cyclic, nucleotide, PDE, inhibitors, inhibits, non-selective, adenosines, receptors, phosphodiesterases, ryanodine, Non-selective, Adenosine, Phosphodiesterases, Ryanodine, Receptors, 2793, Tocris Bioscience

2 篇 Caffeine 的引用文献

引用文献是使用了 Tocris 产品的出版物。 Caffeine 的部分引用包括：

Okita et al (2015) Contrasting Effects of the Cytotoxic Anticancer Drug gemcit. and the EGFR Tyrosine Kinase Inhibitor gefi. on NK Cell-Mediated Cytotoxicity via Regulation of NKG2D Ligand in Non-Small-Cell Lung Cancer Cells. PLoS One 10 e0139809 PMID: 26439264

Seto et al (2013) Acute SimV. inhibits K ATP channels of porcine coronary artery myocytes. J Cell Sci 8 e66404 PMID: 23799098

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Caffeine 的评论

平均评分: 4.7 (基于 3 条评论。)

5 星
67%
4 星
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3 星
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Low-dose oral caffeine induces a specific form of behavioral sensitization.
By Kevin Ball on 01/28/2019
分析类型： In Vivo
种属： Rat

Rats had free access to untreated water or water containing 0.2 mg/ml of caffeine every other day of a 14-day experiment. This dose is relevant to human caffeine intake. Rats were tested on alternated days in an open-field arena for discrete movements (horizontal and vertical) and ambulatory distance. Results showed that although caffeine intake decreased significantly across test sessions, the number of discrete horizontal movements increased significantly, that is, showed sensitization.

PMID: 22358105 参考文献
review image
Caffeine acts on RyR2 in HL1 cells to release ER Ca2+..
By Anonymous on 12/16/2018
分析类型： In Vitro
种属： Mouse
细胞系/组织： HL-1 Atrial Muscle Cell Line

HL-1 cells were loaded with cytosolic Ca2+ sensitive dye, Fura-2AM (1 µM)+F127 (0.01%) in normal tyrode solution for 40 min and excited alternately at 340 and 380 nm and fluorescence was captured at 510 nm. When the cells are perfused with 10 mM caffeine in nominally Ca2+ free tyrode solution, caffeine acts on RyR2 to release ER Ca2+, resulting in transient cytosolic peak (shown in the figure).

review image
Caffeine as a tool to estimate sarcoplasmic Ca2+ content in cardiomyocytes.
By Christopher Trummer on 10/15/2018
分析类型： In Vitro
种属： Other
细胞系/组织： primary ventricular cardiomyocytes

Control or saxagliptin treated primary guinea pig cardiomyocytes were loaded with FURA-2AM (1 µM) and Pluronic F 127 (1%). Ca2+ transient decay tau evoked by caffeine (30 µM) is shown in the Image. Caffeine acts as a ryanodine receptor agonist and releases Ca2+ from sarcoplasmic reticulum into the cytosol.

review image