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Submit ReviewCyclopamine
说明： Inhibitor of Hedgehog (Hh) signaling
化学名： (2'R,3S,3'R,3'aS,6'S,6aS,6bS,7'aR,11aS,11bR)-1,2,3,3'a,4,4',5',6,6',6a,6b,7,7',7'a,8,11,11a,11b-Octadecahydro-3',6',10,11b-tetramethylspiro[9H-benzo[a]fluorene-9,2'(3'H)-furo[3,2-b]pyridin]-3-ol
纯度： ≥97% (HPLC)
生物活性 for Cyclopamine
Cyclopamine is an inhibitor of hedgehog (Hh) signaling, likely via direct inhibition of Smoothened, the accessory protein to the putative Hh receptor Patched. Anticancer and teratogenic in vivo. Depletes stem-like cancer cells in glioblastoma and blocks tumor engraftment. Can also be used to induce differentiation of human embryonic stem cells (hESCs) into hormone-expressing endocrine cells.
技术数据 for Cyclopamine
| 分子量 | 411.63 |
| 公式 | C27H41NO2 |
| 储存 | Desiccate at -20°C |
| 纯度 | ≥97% (HPLC) |
| CAS Number | 4449-51-8 |
| PubChem ID | 442972 |
| InChI Key | QASFUMOKHFSJGL-LAFRSMQTSA-N |
| Smiles | O[C@H](C2)CC[C@@]1(C)C2=CC[C@]([C@@]([H])3CC[C@@]54O[C@@](C[C@H](C)CN6)([H])[C@]6([H])[C@H]5C)([H])[C@@]([H])1CC3=[C@@]4C |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Cyclopamine
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| ethanol | 2.06 | 5 |
制备储备液 for Cyclopamine
以下数据基于产品分子量 411.63。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.05 mM | 48.59 mL | 242.94 mL | 485.87 mL |
| 0.25 mM | 9.72 mL | 48.59 mL | 97.17 mL |
| 0.5 mM | 4.86 mL | 24.29 mL | 48.59 mL |
| 2.5 mM | 0.97 mL | 4.86 mL | 9.72 mL |
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产品说明书 for Cyclopamine
参考文献 for Cyclopamine
参考文献是支持产品生物活性的出版物。
Chen et al (2002) Inhibition of hedgehog signaling by direct binding of cyclopamine to smoothened. Genes Dev. 16 2743 PMID: 12414725
Scott (2003) Cancer: a twist in a hedgehog's tale. Nature 425 780 PMID: 14574396
Bar et al (2007) Cyclopamine-mediated hedgehog pathway inhibition depletes stem-like cancer cells in glioblastoma. Stem Cells 25 2524 PMID: 17628016
D'Amour et al (2006) Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat.Biotechnol. 24 1392 PMID: 17053790
If you know of a relevant reference for Cyclopamine, please let us know.
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关键词: Cyclopamine, Cyclopamine supplier, inhibitors, inhibits, hedgehog, Hh, signaling, Signalling, Hedgehog, stem, cells, Smo, smoothened, antagonist, Signaling, Stem, Cell, Smoothened, Receptors, Cancer, Cells, ESCs, and, iPSC, 1623, Tocris Bioscience
8 篇 Cyclopamine 的引用文献
引用文献是使用了 Tocris 产品的出版物。 Cyclopamine 的部分引用包括：
Yuan et al (2016) Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation. Stem Cells Int 7 11024 PMID: 26996322
Atzmon et al (2018) Drug Screening Identifies Sigma-1-Receptor as a Target for the Therapy of VWM Leukodystrophy. Front Mol Neurosci 11 336 PMID: 30279648
Yan et al (2017) Chemical inhibition reveals differential requirements of signaling pathways in krasV12- and Myc-induced liver tumors in transgenic zebrafish. Sci Rep 7 45796 PMID: 28378824
Rodrigues (2017) Integration of Shh and Fgf signaling in controlling Hox gene expression in cultured limb cells. PNAS 114 3139 PMID: 28270602
Atwood et al (2013) GLI activation by atypical protein kinase C Ι/λ regulates the growth of basal cell carcinomas. Nat Commun 494 484 PMID: 23446420
Zou et al (2015) Sonic hedgehog produced by bone marrow-derived mesenchymal stromal cells supports cell survival in myelodysplastic syndrome. Sci Signal 2015 957502 PMID: 25861282
Moisan et al (2015) White-to-brown metabolic conversion of human adipocytes by JAK inhibition. Nat Cell Biol 17 57 PMID: 25487280
Kumar et al (2015) Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells. Elife 4 PMID: 26554899
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Cyclopamine 的评论
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Modulators of Hh signalling and stress fibres.
By
Anonymous on 11/09/2019
分析类型： In Vitro
种属： Human
细胞系/组织： Primary OPCs
Modulators of Hh signalling and stress fibres. Cyclopamine (Tocris Bioscience, 1623) was dissolved in ethanol and DMSO. The final concentration used for cells was 5 mM of cyclopamine.
该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Stem Cell Research Product Guide
This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:
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Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.