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Submit ReviewKyoto Probe-1
说明： Fluorescent probe that selectively identifies undifferentiated iPS/ES cells
别名： KP1
化学名： 9-(4-Fluorophenyl)-3-imino-3H-xanthen-6-amine trifluoroacetate
纯度： ≥95% (HPLC)
生物活性 for Kyoto Probe-1
Kyoto Probe-1 distinguishes between undifferentiated iPS/ES cells and differentiated cells, so may be used for monitoring pluripotency during hESC or iPSC maintenance. Selectivity of labeling is due to ATP-binding cassette transporters ABCB1 and ABCG2 - these mediate efflux of Kyoto Probe-1 and are expressed in differentiated cells, but are repressed in hiPSC and hESC. Kyoto Probe-1 distinguishes between SSEA-4-positive hESCs and human early hematopoietic cells expressing CD45, CD235, CD41a, or CD43 in flow cytometry experiments, making it a valuable tool for studying early hematopoiesis. The product also distinguishes between hiPSCs and hiPSC-derived cardiomyocytes. Note that Kyoto Probe-1 does not differentiate between hiPSCs and differentiated human neuronal stem cells.
Kyoto Probe-1 localizes to the mitochondria in undifferentiated iPS/ES cells only. Suitable in flow cytometry and live cell imaging.
Excitation maximum = 515 nm; emission maximum = 529 nm; Quantum yield = 0.45
许可信息
Sold under license from New York University
Optical Data for Kyoto Probe-1
| Emission Color | Green |
|---|---|
| λabs | 515 nm |
| λem | 529 nm |
| Quantum Yield (φ) | 0.45 |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Live-cell Imaging |
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技术数据 for Kyoto Probe-1
| 分子量 | 418.35 |
| 公式 | C19H13FN2O.CF3CO2H |
| 储存 | Store at -20°C |
| 纯度 | ≥95% (HPLC) |
| CAS Number | 2088021-81-0 |
| InChI Key | ADFLZJALCLDWDM-UHFFFAOYSA-N |
| Smiles | [O-]C(C(F)(F)F)=O.[NH2+]=C1C=C2OC3=C(C(C4=CC=C(C=C4)F)=C2C=C1)C=CC(N)=C3 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
溶解性数据 for Kyoto Probe-1
| 溶剂 | 最高浓度 mg/mL | 最高浓度 mM | |
|---|---|---|---|
| 溶解性 | |||
| DMSO | 41.83 | 100 |
制备储备液 for Kyoto Probe-1
以下数据基于产品分子量 418.35。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| 浓度/溶剂体积/质量 | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.39 mL | 11.95 mL | 23.9 mL |
| 5 mM | 0.48 mL | 2.39 mL | 4.78 mL |
| 10 mM | 0.24 mL | 1.2 mL | 2.39 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.48 mL |
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产品说明书 for Kyoto Probe-1
参考文献 for Kyoto Probe-1
参考文献是支持产品生物活性的出版物。
Mao et al (2017) A synthetic hybrid molecule for the selective removal of human pluripotent stem cells from cell mixtures. Angew.Chem.Int.Ed.Engl. 56 1765 PMID: 28067441
Hirata et al (2014) A chemical probe that labels human pluripotent stem cells. Cell Rep. 6 1165 PMID: 24613351
Miyagi-Shiohira et al (2020) Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells. Sci.Rep. 10 18084 PMID: 33093580
If you know of a relevant reference for Kyoto Probe-1, please let us know.
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该领域的文献
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*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
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Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
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