Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewLOC14
说明： High affinity protein disulfide isomerase (PDI) modulator
化学名： 2-[[4-(Cyclopropylcarbonyl)-1-piperazinyl]methyl]-1,2-benzisothiazol-3(2H)-one
纯度： ≥98% (HPLC)
生物活性 for LOC14
LOC14 is a high affinity allosteric inhibitor of protein disulfide isomerase A3 (PDIA3) modulator (Kd = 62 nM, IC50 = 5 μM). In an animal model of Huntington's disease LOC14 extends survival, improves motor function, reduces ER stress from mHTT and preserves dopamine and DARPP32 levels. LOC14 is neuroprotective and rescues medium spiny neurons from neurodegeneration. LOC14 also decreases influenza A protein production in cells (IC50 = 4.97 μM).
技术数据 for LOC14
| 分子量 | 317.41 |
| 公式 | C16H19N3O2S |
| 储存 | Store at +4°C |
| 纯度 | ≥98% (HPLC) |
| CAS Number | 877963-94-5 |
| PubChem ID | 9117962 |
| InChI Key | YVBSNHLFRIVWFQ-UHFFFAOYSA-N |
| Smiles | O=C1C2=CC=CC=C2SN1CN3CCN(C(C4CC4)=O)CC3 |
上方提供的技术数据仅供参考。批次相关数据请参见分析证书。
Tocris products are intended for laboratory research use only, unless stated otherwise.
产品说明书 for LOC14
参考文献 for LOC14
参考文献是支持产品生物活性的出版物。
Kaplan et al (2015) Small molecule-induced oxidation of protein disulfide isomerase is neuroprotective. Proc.Natl.Acad.Sci.U.S.A. 112 E2245 PMID: 25848045
Zhou et al (2018) Small molecule modulator of protein disulfide isomerase attenuates mutant huntingtin toxicity and inhibits endoplasmic reticulum stress in a mouse model of Huntington's disease. Hum.Mol.Genet. 27 1545 PMID: 29462355
Chamberlain et al (2019) Lung epithelial protein disulfide isomerase A3 (PDIA3) plays an important role in influenza infection, inflammation, and airway mechanics. Redox.Biol. 22 PMID: 30735910
按标靶查看相关产品
按产品操作查看相关产品
关键词: LOC14, LOC14 supplier, LOC14, High, affinity, protein, disulfide, isomerase, PDI, allosteric, modulator, Huntington, disease, Protein, Disulfide, Isomerase, 5606, Tocris Bioscience
2 篇 LOC14 的引用文献
引用文献是使用了 Tocris 产品的出版物。 LOC14 的部分引用包括：
Chamberlain et al (2019) Lung epithelial protein disulfide isomerase A3 (PDIA3) plays an important role in influenza infection, inflammation, and airway mechanics. Redox Biol 22 101129 PMID: 30735910
Vikas et al (2022) Protein Disulfide Isomerase A3 Regulates Influenza Neuraminidase Activity and Influenza Burden in the Lung. Int J Mol Sci 23 PMID: 35162999
LOC14 的评论
平均评分: 5 (Based on 1 Review.)
Have you used LOC14?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
PDI inhibitor LOC14 inhibits platelet spreading over fibrinogen.
By
Renato Gaspar on 05/11/2019
分析类型： Ex Vivo
种属： Human
细胞系/组织： Platelets
Washed platelets (2 x 10ˆ7 platelets/mL) were incubated in absence or presence of LOC14 (0.1, 1 and 10 µM) for 10 minutes at 37°C, then 300 µL of the solution was dispensed on a fibrinogen (100 µg/mL)-coated coverslip for 45 minutes at 37°C. Non-adhered platelets were removed and the coverslip washed three times with PBS. Adhered platelets were fixed using 0.2% paraformaldehyde for 10 minutes. This solution was then removed and coverslips washed three times with PBS before the addition of 0.1% (v/v) Triton-X to permeabilise the cells. After removal of Triton-X and further washes using PBS, platelets were stained with Alexa Fluor 488-conjugated phalloidin (1:1000 v/v) for 1 hour in the dark at room temperature. Coverslips were mounted onto microscope glass slides and imaged using a 100x oil immersion lens on a Nikon A1-R Confocal microscope.
该领域的文献
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*请注意，Tocris 仅会向正规科研企业/机构地址发送文献。
Gut Hormones Poster
The gastrointestinal tract is the largest endocrine gland in the human body, secreting hormones that affect digestion, appetite and energy expenditure. Neuropeptide modulators and gut hormones that influence appetite are reviewed in this poster.