Methotrexate

Cat. No. 1230 2 篇引用文献提交评论说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

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说明： Cytotoxic agent

化学名： N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methylamino]benzoyl]-L-glutamic acid

纯度： ≥99% (HPLC)

引用文献 (2)

文献 (2)

生物活性技术数据溶解性计算器说明书参考文献

生物活性 for Methotrexate

Methotrexate is a cytotoxic agent. Inhibits thymidylate synthetase and de novo purine synthesis. Potent folic acid antagonist; inhibits dihydrofolate reductase. Also inhibits Ras carboxyl methylation in DKOB8 cells, leading to decreased p44 and Akt activation.

技术数据 for Methotrexate

分子量	454.44
公式	C₂₀H₂₂N₈O₅
储存	Store at RT
纯度	≥99% (HPLC)
CAS Number	59-05-2
PubChem ID	126941
InChI Key	FBOZXECLQNJBKD-ZDUSSCGKSA-N
Smiles	O=C(N[C@H]([C@](O)=O)CCC(O)=O)C(C=C3)=CC=C3N(CC2=NC1=C(N)N=C(N)N=C1N=C2)C

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

溶解性数据 for Methotrexate

	溶剂	最高浓度 mg/mL	最高浓度 mM
溶解性
溶解性	DMSO		100

制备储备液 for Methotrexate

以下数据基于产品分子量 454.44。 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

浓度/溶剂体积/质量	1 mg	5 mg	10 mg
1 mM	2.2 mL	11 mL	22.01 mL
5 mM	0.44 mL	2.2 mL	4.4 mL
10 mM	0.22 mL	1.1 mL	2.2 mL
50 mM	0.04 mL	0.22 mL	0.44 mL

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产品说明书 for Methotrexate

分析证书/产品说明书

化学品安全技术说明书

参考文献 for Methotrexate

参考文献是支持产品生物活性的出版物。

Allegra et al (1987) Evidence for direct inhibition of de novo protein synthesis in human MCF-7 breast cells as a principal mode of metabolic inhibition by methotr. J.Biol.Chem. 262 13520 PMID: 2443493

Chu et al (1990) Mechanism of thymidylate synthase inhibition by methotr. in human neoplastic cell lines and normal human myeloid progenitor cells. J.Biol.Chem. 265 8470 PMID: 2341391

Jolivet et al (1983) The pharmacology and clinical use of methotr. N.Engl.J.Med. 309 1094 PMID: 6353235

Winter-Vann et al (2003) Targeting Ras signaling through inhibition of carboxyl methylation: an unexpected property of methotr. Proc.Natl.Acad.Sci.U.S.A. 100 6529 PMID: 12750467

If you know of a relevant reference for Methotrexate, please let us know.

按标靶查看相关产品

关键词: Methotrexate, Methotrexate supplier, Cytotoxic, agent, Dihydrofolate, reductases, inhibitors, inhibits, Cell, Cycle, DHFR, chemotherapeutics, Reductase, Inhibitors, 1230, Tocris Bioscience

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

2 篇 Methotrexate 的引用文献

引用文献是使用了 Tocris 产品的出版物。 Methotrexate 的部分引用包括：

Kumar et al (2018) Comparison of cell-based assays to quantify treatment effects of anticancer drugs identifies a new application for Bodipy-L-cystine to measure apoptosis. Sci Rep 8 16363 PMID: 30397244

Robinson et al (2013) RB1 status in triple negative breast cancer cells dictates response to radiation treatment and selective therapeutic drugs. PLoS One 8 e78641 PMID: 24265703

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