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MitoMark Green I

Cat. No. 6444 提交评论 说明书 / 分析证书（CoA） / 化学品安全技术说明书（SDS）

Discontinued Product

6444 has been discontinued.

View all Other Mitochondria Dyes and Probes products.
说明： Green fluorescent mitochondrial stain; cell permeable
化学名： 2-[3-[5,6-Dichloro-1,3-bis[[4-(chloromethyl)phenyl]methyl]-1,3-dihydro-2H-benzimidazol-2-ylidene]-1-propen-1-yl]-3-methylbenzoxazolium chloride
纯度： ≥85% (HPLC)
说明书
引用文献
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文献 (1)
生物活性 Scientific Data 技术数据 说明书 参考文献

生物活性 for MitoMark Green I

MitoMark Green I is a green fluorescent mitochondrial stain. Localizes to mitochondria regardless of mitochondrial membrane potential. Excitation/emission maxima λ ~ 490/516 nm.

It is recommended to prepare stock solutions in DMSO.

Scientific Data

Fluorescence Imaging Applications in HeLa cells for MitoMark Green I View Larger

Applications in HeLa cells for MitoMark Green I. Application of MitoMark Green I: MitoMark Green I was diluted in HBSS and applied to live HeLa cells at a concentration of 200 nM. HeLa cells were incubated for 30 minutes at 37°C. After incubation was complete, MitoMark Green I dilutions were washed off, and fresh HBSS was applied to the cells. Cells were not fixed because MitoMark Green I staining is not retained well after fixation (photobleach). Live cell staining is optimal for MitoMark Green I.

Optical Data for MitoMark Green I

Emission Color Green
λabs 490 nm
λem 516 nm
Extinction Coefficient (ε) 119000 M-1cm-1

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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技术数据 for MitoMark Green I

分子量 671.87
公式 C34H28Cl5N3O
储存 Store at -20°C
纯度 ≥85% (HPLC)
CAS Number 201860-17-5
PubChem ID 77913543
InChI Key RUVJFMSQTCEAAB-UHFFFAOYSA-M
Smiles ClC1=CC2=C([N+](CC3=CC=C(CCl)C=C3)=C(/C=C/C=C4N(C)C5=CC=CC=C5O\4)N2CC6=CC=C(CCl)C=C6)C=C1Cl.[Cl-]

上方提供的技术数据仅供参考。批次相关数据请参见分析证书。

Tocris products are intended for laboratory research use only, unless stated otherwise.

产品说明书 for MitoMark Green I

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化学品安全技术说明书

参考文献 for MitoMark Green I

参考文献是支持产品生物活性的出版物。

Garner et al (1997) Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biol.Reprod. 57 1401 PMID: 9408246

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关键词: MitoMark Green I, MitoMark Green I supplier, MitoMark, green, mitochondrial, marker, stain, mitotracker, mitochondria, Organelle, and, Cell, Structure, Stains, Labels, Flow, Cytometry, Other, Mitochondria, Dyes, Probes, 6444, Tocris Bioscience

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该领域的文献

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Fluorescent Dyes and Probes Research Product Guide

Fluorescent Dyes and Probes Research Product Guide

This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:

  • Fluorescent Dyes, including dyes for flow cytometry
  • Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
  • Tissue Clearing Kits and Reagents
  • Aptamer-based RNA Imaging Reagents
  • Fluorescent Probes for Imaging Bacteria
  • TSA VividTM Fluorophore Kits
  • TSA Reagents for Enhancing IHC, ICC & FISH Signals