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Submit ReviewNecrosulfonamide
Description: Necroptosis inhibitor; also inhibits pyroptosis
Chemical Name: (2E)-N-[4-[[(3-Methoxy-2-pyrazinyl)amino]sulfonyl]phenyl]-3-(5-nitro-2-thienyl)-2-propenamide
Purity: ≥98% (HPLC)
Biological Activity for Necrosulfonamide
Necrosulfonamide is a necroptosis inhibitor. Blocks mixed lineage kinase domain-like protein (MLKL), a critical substrate of receptor-interacting serine-threonine kinase 3 (RIP3) during necrosis. Prevents MLKL-RIP1-RIP3 necrosome complex from interacting with downstream necrosis effectors. Binds and inhibits gasdermin D. Inhibits pyroptosis.
Technical Data for Necrosulfonamide
| M. Wt | 461.47 |
| Formula | C18H15N5O6S2 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1360614-48-7 |
| PubChem ID | 1566236 |
| InChI Key | FNPPHVLYVGMZMZ-XBXARRHUSA-N |
| Smiles | O=C(NC2=CC=C(S(NC3=C(OC)N=CC=N3)(=O)=O)C=C2)/C=C/C1=CC=C([N+]([O-])=O)S1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Necrosulfonamide
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 9.23 | 20 |
Preparing Stock Solutions for Necrosulfonamide
The following data is based on the product molecular weight 461.47. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 10.83 mL | 54.17 mL | 108.35 mL |
| 1 mM | 2.17 mL | 10.83 mL | 21.67 mL |
| 2 mM | 1.08 mL | 5.42 mL | 10.83 mL |
| 10 mM | 0.22 mL | 1.08 mL | 2.17 mL |
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Product Datasheets for Necrosulfonamide
References for Necrosulfonamide
References are publications that support the biological activity of the product.
Zhou et al (2012) New components of the necroptotic pathway. Protein Cell 3 811 PMID: 23073834
Sun et al (2012) Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148 213 PMID: 22265413
Kreuzaler et al (2012) Killing a cancer: what are the alternatives? Nat.Rev.Cancer. 12 411 PMID: 22576162
Pandeya et al (2019) Gasdermin D (GSDMD) as a new target for the treatment of infection. Medchemcomm 10 660 PMID: 31191857
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Keywords: Necrosulfonamide, Necrosulfonamide supplier, non-apoptotic, cell, death, necroptosis, necrosis, necrosome, mixed, lineage, kinase, domain-like, protein, MLKL, receptor, interacting, serine, threonine, kinases, 3, RIP3, Gasdermin, D, GSDMD, pyroptosis, inhibitors, inhibits, Necroptosis, Inflammasomes, 5025, Tocris Bioscience
19 Citations for Necrosulfonamide
Citations are publications that use Tocris products. Selected citations for Necrosulfonamide include:
Bittner et al (2016) Death receptor 3 mediates necroptotic cell death. Cellular and Molecular Life Sciences PMID: 27592300
Junying et al (2021) RIPK1 Promotes Energy Sensing by the mTORC1 Pathway. Mol Cell 81 370-385.e7 PMID: 33271062
Ferenc et al (2021) Caspase-9 acts as a regulator of necroptotic cell death. FEBS J 288 6476-6491 PMID: 33899329
Michael E et al (2021) Overexpression screen of interferon-stimulated genes identifies RARRES3 as a restrictor of Toxoplasma gondii infection. Elife 10 PMID: 34871166
Heulot et al (2016) The TAT-RasGAP317-326 anti-cancer peptide can kill in a caspase-, apoptosis-, and necroptosis-independent manner. Oncotarget 7 64342 PMID: 27602963
Svetlana et al (2022) A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling. Cell 185 2354-2369.e17 PMID: 35568036
Gonzalez-Juarbe et al (2017) Pore-forming toxin-mediated ion dysregulation leads to death receptor-independent necroptosis of lung epithelial cells during bacterial pneumonia. Cell Death Differ 24 917 PMID: 28387756
Xie et al (2017) The Tumor Suppressor p53 Limits Ferroptosis by Blocking DPP4 Activity. Cell Rep 20 1692 PMID: 28813679
Charles E et al (2019) Upregulation of human glycolipid transfer protein (GLTP) induces necroptosis in colon carcinoma cells. Biochim Biophys Acta Mol Cell Biol Lipids 1864 158-167 PMID: 30472325
Kevin S et al (2020) Influenza-Induced Oxidative Stress Sensitizes Lung Cells to Bacterial-Toxin-Mediated Necroptosis. Cell Rep 32 108062 PMID: 32846120
Tanya A et al (2020) Lytic Cell Death Mechanisms in Human Respiratory Syncytial Virus-Infected Macrophages: Roles of Pyroptosis and Necroptosis. Viruses 12 PMID: 32854254
Kyung-Min et al (2020) Combined the SMAC mimetic and BCL2 inhibitor sensitizes neoadjuvant chemotherapy by targeting necrosome complexes in tyrosine aminoacyl-tRNA synthase-positive breast cancer. Breast Cancer Res 22 130 PMID: 33239070
James et al (2020) Locking mixed-lineage kinase domain-like protein in its auto-inhibited state prevents necroptosis. Proc Natl Acad Sci U S A 117 33272-33281 PMID: 33318170
Simon et al (2023) PRMT5-mediated regulatory arginine methylation of RIPK3. Cell Death Discov 9 14 PMID: 36658119
Yuan et al (2023) Necroptotic kinases are involved in the reduction of depression-induced astrocytes and fluoxetine's inhibitory effects on necroptotic kinases. Front Pharmacol 13 1060954 PMID: 36686688
Gonzalez-Juarbe et al (2015) Pore-Forming Toxins Induce Macrophage Necroptosis during Acute Bacterial Pneumonia. Channels (Austin) 11 e1005337 PMID: 26659062
Greer et al (2018) ONC201 kills breast cancer cells in vitro by targeting mitochondria. Oncotarget 9 18454 PMID: 29719618
Chefetz et al (2019) A Pan-ALDH1A Inhibitor Induces Necroptosis in Ovarian Cancer Stem-like Cells. Cell Rep 26 3061 PMID: 30865894
Bagnjuk et al (2019) Necroptosis in primate luteolysis: a role for ceramide. Cell Death Discov 5 67 PMID: 30774995
Do you know of a great paper that uses Necrosulfonamide from Tocris? Please let us know.
Reviews for Necrosulfonamide
Average Rating: 5 (Based on 1 Review.)
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Necrosulfonamide work well to block pyroptosis.
By
Xiaogang Wang on 06/12/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: THP-1 cell line
We used Necrosulfonamide as a positive control to inhibit the induction of pyroptosis by staphylococcal cytolysin. Differentiated THP-1 cells were pretreated with 25uM Necrosulfonamide (NSA) for 1 h before treating with or without 1 ug/ml Staphylococcal pore-forming toxin for 4 h. The LDH release in culture supernatants of treated cells was measured. Our data indicated that Necrosulfonamide treatment can significantly inhibit staphylococcal cytolysin induced pyroptosis.
Literature in this Area
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Programmed Cell Death Poster
There are two currently recognized forms of programmed cell death: apoptosis and necroptosis. This poster summarizes the signaling pathways involved in apoptosis, necroptosis and cell survival following death receptor activation, and highlights the influence of the molecular switch, cFLIP, on cell fate.
Pathways for Necrosulfonamide
