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Submit ReviewAY 9944 dihydrochloride
Description: Inhibitor of Hedgehog (Hh) signaling. Inhibits Δ7-dehydrocholesterol reductase
Chemical Name: trans-N,N-bis[2-Chlorophenylmethyl]-1,4-cyclohexanedimethanamine dihydrochloride
Purity: ≥99% (HPLC)
Biological Activity for AY 9944 dihydrochloride
AY 9944 dihydrochloride is an inhibitor of hedgehog (Hh) signaling, possibly via several mechanisms. Inhibits Δ7-dehydrocholesterol reductase (IC50 = 13 nM), thus reduces cholesterol biosynthesis, and also inhibits cholesterol esterification. May also directly block the cellular response to Hh proteins. Teratogenic in vivo.
Technical Data for AY 9944 dihydrochloride
| M. Wt | 464.3 |
| Formula | C22H28Cl2N2.2HCl |
| Storage | Desiccate at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 366-93-8 |
| PubChem ID | 9954082 |
| InChI Key | GZFYZYBWLCYBMI-MYZJJQSMSA-N |
| Smiles | ClC(C=CC=C3)=C3CNC[C@H]1CC[C@H](CNCC2=C(Cl)C=CC=C2)CC1.Cl.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for AY 9944 dihydrochloride
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 23.21 | 50 | |
| DMSO | 2.32 | 5 |
Preparing Stock Solutions for AY 9944 dihydrochloride
The following data is based on the product molecular weight 464.3. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 4.31 mL | 21.54 mL | 43.08 mL |
| 2.5 mM | 0.86 mL | 4.31 mL | 8.62 mL |
| 5 mM | 0.43 mL | 2.15 mL | 4.31 mL |
| 25 mM | 0.09 mL | 0.43 mL | 0.86 mL |
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Product Datasheets for AY 9944 dihydrochloride
References for AY 9944 dihydrochloride
References are publications that support the biological activity of the product.
Cooper et al (1998) Teratogen-mediated inhibition of target tissue response to Shh signaling. Science 280 1603 PMID: 9616123
Incardona and Eaton (2000) Cholesterol in signal transduction. Curr.Opin.Cell Biol. 12 193 PMID: 10712926
Kraml et al (1964) Inhibition of the conversion of 7-dehydrocholesterol to cholesterol by AY-9944. Biochem.Biophys.Res.Commun. 15 455 PMID: 4283982
Moebius et al (1998) Molecular cloning and expression of the human Δ7-sterol reductase. Proc.Natl.Acad.Sci.U.S.A. 95 1899 PMID: 9465114
If you know of a relevant reference for AY 9944 dihydrochloride, please let us know.
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1 Citation for AY 9944 dihydrochloride
Citations are publications that use Tocris products. Selected citations for AY 9944 dihydrochloride include:
Kant et al (2019) Cholesterol Metabolism Is a Druggable Axis that Independently Regulates Tau and Amyloid-β in iPSC-Derived Alzheimer's Disease Neurons. Cell Stem Cell 24 363 PMID: 30686764
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