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Submit ReviewCaffeine
Description: A1 and A2B antagonist. CNS stimulant
Chemical Name: 3,7-Dihydro-1,3,7-trimethyl-1H-purine-2,6-dione
Purity: ≥99% (HPLC)
Biological Activity for Caffeine
Caffeine is a central nervous system stimulant. Antagonist at A1 and A2A adenosine receptors and inhibitor of cyclic nucleotide phosphodiesterases. Mobilizes calcium from intracellular stores and inhibits benzodiazepine binding to GABA receptors.
Technical Data for Caffeine
| M. Wt | 194.19 |
| Formula | C8H10N4O2 |
| Storage | Store at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 58-08-2 |
| PubChem ID | 2519 |
| InChI Key | RYYVLZVUVIJVGH-UHFFFAOYSA-N |
| Smiles | O=C1C2=C(N=CN2C)N(C)C(N1C)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Caffeine
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 19.42 | 100 | |
| DMSO | 9.71 | 50 |
Preparing Stock Solutions for Caffeine
The following data is based on the product molecular weight 194.19. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 5.15 mL | 25.75 mL | 51.5 mL |
| 5 mM | 1.03 mL | 5.15 mL | 10.3 mL |
| 10 mM | 0.51 mL | 2.57 mL | 5.15 mL |
| 50 mM | 0.1 mL | 0.51 mL | 1.03 mL |
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Product Datasheets for Caffeine
References for Caffeine
References are publications that support the biological activity of the product.
Nehlig et al (1992) Caffeine and the central nervous system: mechanisms of action, biochemical, metabolic and psychostimulant effects. Brain Res.Brain Res.Rev. 17 139 PMID: 1356551
Fisone et al (2004) Caffeine as a psychomotor stimulant: mechanism of action. Cell.Mol.Life Sci. 61 857 PMID: 15095008
Chen et al (2008) Caffeine blocks disruption of blood brain barrier in a rabbit model of Alzheimer's disease. J.Neuroinflammation 5 12 PMID: 18387175
If you know of a relevant reference for Caffeine, please let us know.
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Keywords: Caffeine, Caffeine supplier, A1, A2B, antagonists, CNS, stimulant, cyclic, nucleotide, PDE, inhibitors, inhibits, non-selective, adenosines, receptors, phosphodiesterases, ryanodine, Non-selective, Adenosine, Phosphodiesterases, Ryanodine, Receptors, 2793, Tocris Bioscience
2 Citations for Caffeine
Citations are publications that use Tocris products. Selected citations for Caffeine include:
Okita et al (2015) Contrasting Effects of the Cytotoxic Anticancer Drug gemcit. and the EGFR Tyrosine Kinase Inhibitor gefi. on NK Cell-Mediated Cytotoxicity via Regulation of NKG2D Ligand in Non-Small-Cell Lung Cancer Cells. PLoS One 10 e0139809 PMID: 26439264
Seto et al (2013) Acute SimV. inhibits K ATP channels of porcine coronary artery myocytes. J Cell Sci 8 e66404 PMID: 23799098
Do you know of a great paper that uses Caffeine from Tocris? Please let us know.
Reviews for Caffeine
Average Rating: (Based on 3 Reviews.)
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