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Description: Chloride channel blocker; TRPV1 modulator; RAD51 recombinase inhibitor
Chemical Name: 4,4'-Diisothiocyanato-2,2'-stilbenedisulfonic acid disodium salt
Purity: ≥95% (HPLC)
Biological Activity for DIDS
DIDS is a ClC-Ka chloride channel blocker (IC50 = 100 μM). Blocks the maxi chloride channel in apical membranes from human placenta. Displays antagonistic activity at TRPM4 and TRPC4 channels; potentiates agonist-induced TRPV1 currents (IC50 = 4.88 μM in rat DRG neurons). Inhibits RAD51 recombinase activity (KD = 2 μM).
Technical Data for DIDS
| M. Wt | 498.48 |
| Formula | C16H8N2Na2O6S4 |
| Storage | Store at +4°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 67483-13-0 |
| PubChem ID | 5702690 |
| InChI Key | GEPAYBXVXXBSKP-SEPHDYHBSA-L |
| Smiles | [Na+].[Na+].[O-]S(=O)(=O)C1=C(\C=C\C2=C(C=C(C=C2)N=C=S)S([O-])(=O)=O)C=CC(=C1)N=C=S |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for DIDS
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| Potassium bicarbonate (0.1M) | 2.49 | 5 |
Preparing Stock Solutions for DIDS
The following data is based on the product molecular weight 498.48. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.05 mM | 40.12 mL | 200.61 mL | 401.22 mL |
| 0.25 mM | 8.02 mL | 40.12 mL | 80.24 mL |
| 0.5 mM | 4.01 mL | 20.06 mL | 40.12 mL |
| 2.5 mM | 0.8 mL | 4.01 mL | 8.02 mL |
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Product Datasheets for DIDS
References for DIDS
References are publications that support the biological activity of the product.
Ishida et al (2009) DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange. Nucleic Acids Res. 37 3367 PMID: 19336413
Wulff (2008) New light on the "old" chloride channel blocker DIDS. ACS Chem.Biol. 3 399 PMID: 18642798
Zhang et al (2012) Agonist-dependent potentiation of vanilloid receptor transient receptor potential vanilloid type 1 function by stilbene derivatives. Mol.Pharmacol. 81 689 PMID: 22328719
Riquelme and Parra et al (1999) Regulation of human placental chloride channel by arachidonic acid and other cis unsaturated fatty acids. Am.J.Obstet.Gynecol. 180 469 PMID: 9988821
If you know of a relevant reference for DIDS, please let us know.
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Keywords: DIDS, DIDS supplier, chloride, channels, ClC-Ka, blockers, trpm4, trpc4, transient, receptor, potential, vanilloid, receptors, trpv1, rad51, homologous, recombination, dna, damage, repair, double, strand, breaks, 4,4-Diisothiocyanato-2,2-stilbenedisulfonic, Other, Chloride, Channels, 4523, Tocris Bioscience
10 Citations for DIDS
Citations are publications that use Tocris products. Selected citations for DIDS include:
Bernhard et al (2019) Functional expression of electrogenic sodium bicarbonate cotransporter 1 (NBCe1) in mouse cortical astrocytes is dependent on S255-257 and regulated by mTOR. Glia 67 2264-2278 PMID: 31318482
Koichi et al (2019) TREK-1 and TRAAK Are Principal K+ Channels at the Nodes of Ranvier for Rapid Action Potential Conduction on Mammalian Myelinated Afferent Nerves. Neuron 104 960-971.e7 PMID: 31630908
Georg et al (2020) Cannabidiol (CBD): a killer for inflammatory rheumatoid arthritis synovial fibroblasts. Cell Death Dis 11 714 PMID: 32873774
Myung-Jin et al (2020) Suppression of CaMKIIβ Inhibits ANO1-Mediated Glioblastoma Progression. Cells 9 PMID: 32357567
Cao et al (2014) SLC17A9 protein functions as a lysosomal ATP transporter and regulates cell viability. BMB Rep 289 23189 PMID: 24962569
Rastogi et al (2021) Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF PLoS Pathog 17 e1009712 PMID: 34324582
Xing-Zhen et al (2021) Ion permeation controlled by hydrophobic residues and proton binding in the proton-activated chloride channel. iScience 24 103395 PMID: 34825147
Matthew et al (2021) Rapid volume pulsation of the extracellular space coincides with epileptiform activity in mice and depends on the NBCe1 transporter. J Physiol 599 3195-3220 PMID: 33942325
Roebber et al (2019) The Role of the Anion in Salt (NaCl) Detection by Mouse Taste Buds. J Neurosci 39 6224 PMID: 31171579
Harper and Poole (2013) Chloride channels are necessary for full platelet phosphatidylserine exposure and procoagulant activity. J Biol Chem 4 e969 PMID: 24357800
Do you know of a great paper that uses DIDS from Tocris? Please let us know.
Reviews for DIDS
Average Rating: 5 (Based on 1 Review.)
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Blocks cellular swelling.
By
Anonymous on 02/06/2018
Assay Type: Ex Vivo
Species: Rat
Used at 250microM-500microM to block cellular swelling in the rat brain slice.