DIDS

Name: DIDS
Brand: Tocris Bioscience
SKU: 4523
Rating: 5 (1 reviews)

Cat. No. 4523 10 Citations 1 Review Submit a Review Datasheet / COA / SDS

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Description: Chloride channel blocker; TRPV1 modulator; RAD51 recombinase inhibitor

Chemical Name: 4,4'-Diisothiocyanato-2,2'-stilbenedisulfonic acid disodium salt

Purity: ≥95% (HPLC)

Datasheet

Citations (10)

Reviews (1)

Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for DIDS

DIDS is a ClC-Ka chloride channel blocker (IC₅₀ = 100 μM). Blocks the maxi chloride channel in apical membranes from human placenta. Displays antagonistic activity at TRPM4 and TRPC4 channels; potentiates agonist-induced TRPV1 currents (IC₅₀ = 4.88 μM in rat DRG neurons). Inhibits RAD51 recombinase activity (K_D = 2 μM).

Technical Data for DIDS

M. Wt	498.48
Formula	C₁₆H₈N₂Na₂O₆S₄
Storage	Store at +4°C
Purity	≥95% (HPLC)
CAS Number	67483-13-0
PubChem ID	5702690
InChI Key	GEPAYBXVXXBSKP-SEPHDYHBSA-L
Smiles	[Na+].[Na+].[O-]S(=O)(=O)C1=C(\C=C\C2=C(C=C(C=C2)N=C=S)S([O-])(=O)=O)C=CC(=C1)N=C=S

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for DIDS

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	Potassium bicarbonate (0.1M)	2.49	5

Preparing Stock Solutions for DIDS

The following data is based on the product molecular weight 498.48. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.05 mM	40.12 mL	200.61 mL	401.22 mL
0.25 mM	8.02 mL	40.12 mL	80.24 mL
0.5 mM	4.01 mL	20.06 mL	40.12 mL
2.5 mM	0.8 mL	4.01 mL	8.02 mL

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Product Datasheets for DIDS

Certificate of Analysis / Product Datasheet

Safety Datasheet

References for DIDS

References are publications that support the biological activity of the product.

Ishida et al (2009) DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange. Nucleic Acids Res. 37 3367 PMID: 19336413

Wulff (2008) New light on the "old" chloride channel blocker DIDS. ACS Chem.Biol. 3 399 PMID: 18642798

Zhang et al (2012) Agonist-dependent potentiation of vanilloid receptor transient receptor potential vanilloid type 1 function by stilbene derivatives. Mol.Pharmacol. 81 689 PMID: 22328719

Riquelme and Parra et al (1999) Regulation of human placental chloride channel by arachidonic acid and other cis unsaturated fatty acids. Am.J.Obstet.Gynecol. 180 469 PMID: 9988821

If you know of a relevant reference for DIDS, please let us know.

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