Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewDonepezil hydrochloride
Description: Potent AChE inhibitor
Alternative Names: E 2020
Chemical Name: 2,3-Dihydro-5,6-dimethoxy-2-[[1-(phenylmethyl)-4-piperidinyl]methyl]-1H-inden-1-one hydrochloride
Purity: ≥99% (HPLC)
Biological Activity for Donepezil hydrochloride
Donepezil hydrochloride is a potent and selective acetylcholinesterase inhibitor (IC50 = 5.7 nM). In animal models of Alzheimer's disease, Donepezil reduces brain tissue Aβ protein and plaque deposition, restores normal glucose metabolism, reduces neuroinflammation and rescues cognitive dysfunction and memory deficits. The pro-cognitive effects are potentiated by combination with Mefloquine (Cat. No. 6819). In vitro Donepezil reduces LPS-induced inflammation and protects against mitochondrial membrane potential damage.
Technical Data for Donepezil hydrochloride
| M. Wt | 415.95 |
| Formula | C24H29NO3.HCl |
| Storage | Desiccate at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 120011-70-3 |
| PubChem ID | 5741 |
| InChI Key | XWAIAVWHZJNZQQ-UHFFFAOYSA-N |
| Smiles | O=C2C(CC3CCN(CC4=CC=CC=C4)CC3)CC1=CC(OC)=C(OC)C=C12.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Donepezil hydrochloride
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 20.8 | 50 |
Preparing Stock Solutions for Donepezil hydrochloride
The following data is based on the product molecular weight 415.95. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 4.81 mL | 24.04 mL | 48.08 mL |
| 2.5 mM | 0.96 mL | 4.81 mL | 9.62 mL |
| 5 mM | 0.48 mL | 2.4 mL | 4.81 mL |
| 25 mM | 0.1 mL | 0.48 mL | 0.96 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for Donepezil hydrochloride
References for Donepezil hydrochloride
References are publications that support the biological activity of the product.
Sugimoto et al (1995) Synthesis and structure-activity relationships of acetylcholinesterase inhibitors: 1-benzyl-4-[(5,6-dimethoxy-1-oxoindan-2-yl)methyl]piperidine hydrochloride and related compounds. J.Med.Chem. 38 4821 PMID: 7490731
Kryger et al (1999) Structure of acetylcholinesterase complexed with E2020 (Aricept): implications for the design of new anti-Alzheimer drugs. Structure 7 297 PMID: 10368299
Sugimoto et al (2000) Donepezil hydrochloride (E2020) and other acetylcholinesterase inhibitors. Curr.Med.Chem. 7 303 PMID: 10637367
Romberg et al (2011) Impaired attention in the 3xTgAD mouse model of Alzheimer's disease: rescue by donepezil (Aricept). J.Neurosci. 31 3500 PMID: 21368062
Droguerre et al (2020) Efficacy of THN201, a combination of donepezil and mefloquine, to reverse neurocognitive deficits in Alzheimer's disease. Front.Neurosci. 14 PMID: 32612499
Hugon et al (2022) Impact of donepezil on brain glucose metabolism assessed using [18F]2-fluoro-2-deoxy-D-glucose positron emission tomography imaging in a mouse model of Alzheimer's disease induced by intracerebroventricular injection of amyloid-beta peptid Front.Neurosci. 16 PMID: 35281502
Kim et al (2021) Donepezil regulates LPS and Aβ-stimulated neuroinflammation through MAPK/NLRP3 inflammasome/STAT3 signaling. Int.J.Mol.Sci. 22 10637 PMID: 34638977
If you know of a relevant reference for Donepezil hydrochloride, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: Donepezil hydrochloride, Donepezil hydrochloride supplier, acetylcholinesterase, ache, selective, potent, inhibitors, inhibits, E2020, neurodegeneration, alzheimers, amyloid, beta, AB, neuroinflammation, Aβ, E, 2020, Cholinesterases, 4385, Tocris Bioscience
3 Citations for Donepezil hydrochloride
Citations are publications that use Tocris products. Selected citations for Donepezil hydrochloride include:
Morris et al (2015) Network dysfunction in α-synuclein transgenic mice and human Lewy body dementia. Ann Clin Transl Neurol 2 1012 PMID: 26732627
Mercedes et al (2017) The proof-of-concept of ASS234: Peripherally administered ASS234 enters the central nervous system and reduces pathology in a male mouse model of Alzheimer disease. J Psychiatry Neurosci 42 59-69 PMID: 27636528
Rennekamp et al (2016) σ1 receptor ligands control a switch between passive and active threat responses. Nat Chem Biol 12 552 PMID: 27239788
Do you know of a great paper that uses Donepezil hydrochloride from Tocris? Please let us know.
Reviews for Donepezil hydrochloride
There are currently no reviews for this product. Be the first to review Donepezil hydrochloride and earn rewards!
Have you used Donepezil hydrochloride?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Alzheimer's Disease Poster
Alzheimer's disease (AD) is a debilitating and progressive neurodegenerative disease and the most common cause of dementia, affecting approximately 30% of individuals aged over 85 years. This poster summarizes the cellular and molecular mechanisms of AD.