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Submit ReviewFIPI
Description: Phospholipase D inhibitor
Chemical Name: N-[2-[4-(2,3-Dihydro-2-oxo-1H-benzimidazol-1-yl)-1-piperidinyl]ethy]-5-fluoro-1H-indole-2-carboxamide hydrochloride
Purity: ≥98% (HPLC)
Biological Activity for FIPI
FIPI is a potent phosholipase D (PLD) inhibitor (IC50 values are 20 and 25 nM for PLD2 and PLD1 respectively). Ameliorates PLD2-driven suppression of membrane ruffling and cell spreading. Orally available.
Compound Libraries for FIPI
FIPI is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.
Technical Data for FIPI
| M. Wt | 457.93 |
| Formula | C23H24FN5O2.HCl |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1781834-93-2 |
| PubChem ID | 90488864 |
| InChI Key | BNMQCDOCYPGJJD-UHFFFAOYSA-N |
| Smiles | O=C(N2)N(C3CCN(CCNC(C4=CC5=C(C=CC(F)=C5)N4)=O)CC3)C1=C2C=CC=C1.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for FIPI
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 22.9 | 50 |
Preparing Stock Solutions for FIPI
The following data is based on the product molecular weight 457.93. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.5 mM | 4.37 mL | 21.84 mL | 43.67 mL |
| 2.5 mM | 0.87 mL | 4.37 mL | 8.73 mL |
| 5 mM | 0.44 mL | 2.18 mL | 4.37 mL |
| 25 mM | 0.09 mL | 0.44 mL | 0.87 mL |
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Product Datasheets for FIPI
References for FIPI
References are publications that support the biological activity of the product.
Monovich et al (2007) Optimization of halopemide for phospholipase D2 inhibition. Bioorg.Med.Chem.Lett. 17 2310 PMID: 17317170
Su et al (2009) 5-fluoro-2-indolyl des-chlorohalopemide (FIPI), a phospholipase D pharmacological inhibitor that alters cell spreading and inhibits chemotaxis. Mol.Pharmcol. 75 43
If you know of a relevant reference for FIPI, please let us know.
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Keywords: FIPI, FIPI supplier, potent, phospholipases, D, PLD1, PLD2, inhibitors, inhibits, esterases, Phospholipases, 3600, Tocris Bioscience
7 Citations for FIPI
Citations are publications that use Tocris products. Selected citations for FIPI include:
Tove Irene et al (2021) Diacylglycerol kinase and phospholipase D inhibitors alter the cellular lipidome and endosomal sorting towards the Golgi apparatus. Cell Mol Life Sci 78 985-1009 PMID: 32447426
Pitt et al (2017) Neuroprotective astrocyte-derived Ins/IGF-1 stimulates endocytic processing and extracellular release of neuron-bound Aβ oligomers. Mol Biol Cell 28 2623 PMID: 28963439
Sajal et al (2019) Role of PLD-PKCζ signaling axis in p47phox phosphorylation for activation of NADPH oxidase by angiotensin II in pulmonary artery smooth muscle cells. Cell Biol Int 43 678-694 PMID: 30977575
Lin and Liu (2019) Mechanical Stretch Induces mTOR Recruitment and Activation at the Phosphatidic Acid-Enriched Macropinosome in Muscle Cell. Front Cell Dev Biol 7 78 PMID: 31139627
Pastor et al (2014) Analysis of ECs and related compounds in plasma: artifactual isomerization and ex vivo enzymatic generation of 2-MGs. J Lipid Res 55 966 PMID: 24610889
Wang et al (2016) The prostaglandin E2-EP3 receptor axis regulates Anaplasma phagocytophilum-mediated NLRC4 inflammasome activation. PLoS Pathog. 12 e1005803 PMID: 27482714
Bonnefond et al (2015) Calcium signals inhibition sensitizes ovarian carcinoma cells to anti-Bcl-xL strategies through Mcl-1 down-regulation. Int J Mol Sci 20 535 PMID: 25627260
Do you know of a great paper that uses FIPI from Tocris? Please let us know.
Reviews for FIPI
Average Rating: 5 (Based on 2 Reviews.)
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great product.
By
Anonymous on 03/26/2020
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: Hippocampal neuron
100 μM treat 45min
used this drug as an inhibitor.
By
Anonymous on 10/05/2017
Assay Type: In Vitro
Species: Mouse
Cell Line/Tissue: macrophages
Pretreat mouse macrophage cells with this inhibitor prior to the bacterial stimulation.
