Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewHX 531
Description: Potent RXR antagonist
Chemical Name: 4-(7,8,9,10-Tetrahydro-5,7,7,10.10-pentamethyl-2-nitro-5H-benzo[b]naphtho[2,3-e][1,4]-diazepin-12-yl)-benzoic acid
Purity: ≥98% (HPLC)
Biological Activity for HX 531
HX 531 is a potent RXR antagonist (IC50 = 18 nM). Promotes white and brown pre-adipocyte differentiation into white adipocytes. Also inhibits bexarotene-induced brown adipogenic reprogramming of myoblasts.
Technical Data for HX 531
| M. Wt | 483.56 |
| Formula | C29H29N3O4 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 188844-34-0 |
| PubChem ID | 11755040 |
| InChI Key | SXKPGYKPQPYJER-UHFFFAOYSA-N |
| Smiles | CC1(C)C2=C(C=C(N(C)C(C=CC([N+]([O-])=O)=C5)=C5N=C3C4=CC=C(C(O)=O)C=C4)C3=C2)C(C)(C)CC1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for HX 531
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 9.67 | 20 |
Preparing Stock Solutions for HX 531
The following data is based on the product molecular weight 483.56. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 10.34 mL | 51.7 mL | 103.4 mL |
| 1 mM | 2.07 mL | 10.34 mL | 20.68 mL |
| 2 mM | 1.03 mL | 5.17 mL | 10.34 mL |
| 10 mM | 0.21 mL | 1.03 mL | 2.07 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for HX 531
References for HX 531
References are publications that support the biological activity of the product.
Ebisawa et al (1999) Retinoid X receptor-antagonistic diazepinylbenzoic acids. Chem.Pharm.Bull. 47 1778 PMID: 10748721
Alique et al (2006) Vitamin A active metabolite, all-trans retinoic acid, induces spinal cord sensitization. II. Effects after intrathecal administration. Br.J.Pharmacol. 149 65 PMID: 16847438
Suzuki et al (2009) Docosahexaenoic acid induces adipose differentiation-related protein through activation of retinoid X receptor in human choriocarcinoma BeWo cells. Biol.Pharm.Bull. 32 1177 PMID: 19571381
Nie et al (2017) Brown adipogenic reprogramming induced by a small molecule. Cell Rep. 18 624 PMID: 28099842
If you know of a relevant reference for HX 531, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: HX 531, HX 531 supplier, HX531, rxrs, retinoids, x, receptors, antagonists, potent, stem, cell, differentiation, adipocytes, Retinoid, X, Receptor, 3912, Tocris Bioscience
12 Citations for HX 531
Citations are publications that use Tocris products. Selected citations for HX 531 include:
Adriano et al (2018) Toward Minimal Residual Disease-Directed Therapy in Melanoma. Cell 174 843-855.e19 PMID: 30017245
Summermatter et al (2013) Skeletal muscle PGC-1α controls whole-body lactate homeostasis through estrogen-related receptor α-dependent activation of LDH B and repression of LDH A. Proc Natl Acad Sci U S A 110 8738 PMID: 23650363
Wnuk et al (2016) The Crucial Involvement of Retinoid X Receptors in DDE Neurotoxicity. PLoS Biol 29 155 PMID: 26563996
Yang et al (2017) Effects of electroacupuncture and the retinoid X receptor (RXR) signalling pathway on oligodendrocyte differentiation in the demyelinated spinal cord of rats. Acupunct Med 35 122 PMID: 27841975
Nie et al (2017) Brown adipogenic reprogramming induced by a small molecule. Cell.Rep. 18 624 PMID: 28099842
Cherian et al (2015) CINPA1 is an inhibitor of constitutive androstane receptor that does not activate pregnane X receptor. Mol Pharmacol 87 878 PMID: 25762023
Olle et al (2017) Testicular organoid generation by a novel in vitro three-layer gradient system. Biomaterials 130 76-89 PMID: 28364632
Wnuk et al (2017) Benzophenone-3 Impairs Autophagy, Alters Epigenetic Status, and Disrupts Retinoid X Receptor Signaling in Apoptotic Neuronal Cells. Mol Neurobiol PMID: 28815487
Edwards et al (2019) Assessment of total, ligand-induced peroxisome proliferator activated receptor γ ligand activity in serum. Environ Health 18 45 PMID: 31072366
Karen T et al (2020) The novel rexinoid MSU-42011 is effective for the treatment of preclinical Kras-driven lung cancer. Sci Rep 10 22244 PMID: 33335263
Rui et al (2020) Phenotypic landscape of intestinal organoid regeneration. Nature 586 275-280 PMID: 33029001
Ane et al (2021) Retinoic acid signaling is critical during the totipotency window in early mammalian development. Nat Struct Mol Biol 28 521-532 PMID: 34045724
Do you know of a great paper that uses HX 531 from Tocris? Please let us know.
Reviews for HX 531
Average Rating: 5 (Based on 1 Review.)
Have you used HX 531?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Complete ablation of RA-induced transcription in MCF7.
By
Anonymous on 11/02/2019
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: MCF7
HX531 was dissolved to a concentration of 20 mM in DMSO with no solubility issues. Retinoic acid (10 uM), DMSO, or RA + HX531 (1 uM) was added to MCF7 cells for 5 hours following by qPCR analysis of a reporter gene normalized to GAPDH levels. 1 uM of HX531 completely ablated RA induced gene transcription by qPCR. This inhibitor exhibited lower variability than AGN193109, however this was repeated only once, and both inhibitors could completely ablate activity, either alone or in combination. We also added retinal to confirm that our reporter activity was retinoic acid-specific.
No apoptosis has been observed in any of our cells lines at 1 uM
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Retinoid Receptors Scientific Review
Written by Alexander Moise, this review summarizes the nature of retinoid receptors, their isotype and isoform variants and modulation of retinoid signaling. Compounds available from Tocris are listed.