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Description: Induces definitive endoderm formation in mouse and human ESCs
Chemical Name: 1-[2-[(2-Carboxyphenyl)methylene]hydrazide]heptanoic acid
Purity: ≥98% (HPLC)
Biological Activity for IDE 1
IDE 1 is an induces definitive endoderm formation in mouse and human embryonic stem cells (ESCs) (EC50 = 125 nM). Thought to activate the TGF-β signaling pathway; induces Smad2 phosphorylation and increases levels of Nodal expression.
This compound is mixture of E/Z isomers.
Licensing Information
Sold for research purpose under license from Harvard University.
Technical Data for IDE 1
| M. Wt | 306.31 |
| Formula | C15H18N2O5 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1160927-48-9 |
| PubChem ID | 66974738 |
| InChI Key | ABKJCDILEUEJSH-UHFFFAOYSA-N |
| Smiles | OC(=O)CCCCCC(=O)NN=CC1=CC=CC=C1C(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for IDE 1
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| 2eq. NaOH | 30.63 | 100 | |
| DMSO | 30.63 | 100 |
Preparing Stock Solutions for IDE 1
The following data is based on the product molecular weight 306.31. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.26 mL | 16.32 mL | 32.65 mL |
| 5 mM | 0.65 mL | 3.26 mL | 6.53 mL |
| 10 mM | 0.33 mL | 1.63 mL | 3.26 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.65 mL |
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Product Datasheets for IDE 1
References for IDE 1
References are publications that support the biological activity of the product.
Borowiak et al (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4 348 PMID: 19341624
Zaret (2009) Using small molecules to great effect in stem cell differentiation. Cell Stem Cell 4 373 PMID: 19427285
Filby et al (2011) Stimulation of AVNA/Nodal signaling is insufficient to induce definitive endoderm formation of cord blood-derived unrestricted somatic stem cells. Stem Cell Res.Ther. 2 16 PMID: 21463501
If you know of a relevant reference for IDE 1, please let us know.
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3 Citations for IDE 1
Citations are publications that use Tocris products. Selected citations for IDE 1 include:
Marjo et al (2018) Differences in definitive endoderm induction approaches using growth factors and small molecules. J Cell Physiol 233 3578-3589 PMID: 29044512
Xu et al (2015) Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol. Stem Cell Res 6 193 PMID: 26438426
Kearns et al (2013) Generation of organized anterior foregut epithelia from pluripotent stem cells using small molecules. J Cell Mol Med 11 1003 PMID: 23917481
Do you know of a great paper that uses IDE 1 from Tocris? Please let us know.
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cell Research Product Guide
This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:
- Self-renewal and Maintenance
- Differentiation
- Reprogramming
- Organoid Generation
- GMP and Ancillary Material Grade Products
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.