Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewKyoto Probe-1
Description: Fluorescent probe that selectively identifies undifferentiated iPS/ES cells
Alternative Names: KP1
Chemical Name: 9-(4-Fluorophenyl)-3-imino-3H-xanthen-6-amine trifluoroacetate
Purity: ≥95% (HPLC)
Biological Activity for Kyoto Probe-1
Kyoto Probe-1 distinguishes between undifferentiated iPS/ES cells and differentiated cells, so may be used for monitoring pluripotency during hESC or iPSC maintenance. Selectivity of labeling is due to ATP-binding cassette transporters ABCB1 and ABCG2 - these mediate efflux of Kyoto Probe-1 and are expressed in differentiated cells, but are repressed in hiPSC and hESC. Kyoto Probe-1 distinguishes between SSEA-4-positive hESCs and human early hematopoietic cells expressing CD45, CD235, CD41a, or CD43 in flow cytometry experiments, making it a valuable tool for studying early hematopoiesis. The product also distinguishes between hiPSCs and hiPSC-derived cardiomyocytes. Note that Kyoto Probe-1 does not differentiate between hiPSCs and differentiated human neuronal stem cells.
Kyoto Probe-1 localizes to the mitochondria in undifferentiated iPS/ES cells only. Suitable in flow cytometry and live cell imaging.
Excitation maximum = 515 nm; emission maximum = 529 nm; Quantum yield = 0.45
Licensing Information
Sold under license from New York University
Optical Data for Kyoto Probe-1
| Emission Color | Green |
|---|---|
| λabs | 515 nm |
| λem | 529 nm |
| Quantum Yield (φ) | 0.45 |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Live-cell Imaging |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Kyoto Probe-1
| M. Wt | 418.35 |
| Formula | C19H13FN2O.CF3CO2H |
| Storage | Store at -20°C |
| Purity | ≥95% (HPLC) |
| CAS Number | 2088021-81-0 |
| InChI Key | ADFLZJALCLDWDM-UHFFFAOYSA-N |
| Smiles | [O-]C(C(F)(F)F)=O.[NH2+]=C1C=C2OC3=C(C(C4=CC=C(C=C4)F)=C2C=C1)C=CC(N)=C3 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Kyoto Probe-1
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 41.83 | 100 |
Preparing Stock Solutions for Kyoto Probe-1
The following data is based on the product molecular weight 418.35. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.39 mL | 11.95 mL | 23.9 mL |
| 5 mM | 0.48 mL | 2.39 mL | 4.78 mL |
| 10 mM | 0.24 mL | 1.2 mL | 2.39 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.48 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for Kyoto Probe-1
References for Kyoto Probe-1
References are publications that support the biological activity of the product.
Mao et al (2017) A synthetic hybrid molecule for the selective removal of human pluripotent stem cells from cell mixtures. Angew.Chem.Int.Ed.Engl. 56 1765 PMID: 28067441
Hirata et al (2014) A chemical probe that labels human pluripotent stem cells. Cell Rep. 6 1165 PMID: 24613351
Miyagi-Shiohira et al (2020) Kyoto probe-1 reveals phenotypic differences between mouse ES cells and iTS-P cells. Sci.Rep. 10 18084 PMID: 33093580
If you know of a relevant reference for Kyoto Probe-1, please let us know.
View Related Products by Target
Keywords: Kyoto Probe-1, Kyoto Probe-1 supplier, kp-1, kp1, Kyoto, Probe, 1, Fluorescent, probes, markers, selective, identifies, undifferentiated, iPS, ES, cells, induced, pluripotent, stem, embryonic, differentiation, biotracker529, KP1, ESCs, and, iPSC, Cardiomyocyte, Stem, Cells, Other, Differentiation, Products, Probes, Cell, Proliferation, 7419, Tocris Bioscience
Citations for Kyoto Probe-1
Citations are publications that use Tocris products.
Currently there are no citations for Kyoto Probe-1. Do you know of a great paper that uses Kyoto Probe-1 from Tocris? Please let us know.
Reviews for Kyoto Probe-1
There are currently no reviews for this product. Be the first to review Kyoto Probe-1 and earn rewards!
Have you used Kyoto Probe-1?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Stem Cell Research Product Guide
This product guide provides a background to the use of small molecules in stem cell research and lists over 200 products for use in:
- Self-renewal and Maintenance
- Differentiation
- Reprogramming
- Organoid Generation
- GMP and Ancillary Material Grade Products
Stem Cells Scientific Review
Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. Key signaling pathways are highlighted, and the regulation of ES cell self-renewal and somatic cell reprogramming is discussed. Compounds available from Tocris are listed.
Stem Cell Workflow Poster
Stem cells have potential as a source of cells and tissues for research and treatment of disease. This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. Advantages of using small molecules are also highlighted.
Stem Cells Poster
Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids.