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Submit ReviewMito-HE
Description: Red-fluorescent mitochondrial superoxide indicator. Used for: live cell imaging. Application: confocal microscopy, flow cytometry
Chemical Name: [6-(3,8-Diamino-6-phenyl-5(6H)-phenanthridinyl)hexyl]triphenylphosphonium iodide
Purity: ≥80% (HPLC)
Biological Activity for Mito-HE
Key information: Mito-HE is a red fluorescent mitochondrial superoxide indicator in live cells.
Used for: live cell imaging, mitochondrial superoxide detection in Parkinson's and Alzheimer's disease in vitro models.
Application: confocal imaging, flow cytometry.
Properties and Photophysical Data: Selective for mitochondrial superoxides over other reactive oxygen and nitrogen species. Excitation and emission maxima (λ) are 510 nm and 580 nm, respectively; extinction coefficient = 117,000 M-1cm-1.
It is recommended to prepare stock solutions in DMSO.
Optical Data for Mito-HE
| Emission Color | Red |
|---|---|
| λabs | 510 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 11700 M-1cm-1 |
| Cell Permeable | Yes |
| Application | Confocal Imaging, Flow Cytometry |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for Mito-HE
| M. Wt | 759.72 |
| Formula | C43H43IN3P |
| Storage | Store at -20°C |
| Purity | ≥80% (HPLC) |
| CAS Number | 1003197-00-9 |
| PubChem ID | 160231797 |
| InChI Key | NMKOJFMSKHLXRV-UHFFFAOYSA-M |
| Smiles | NC1=CC=C2C(C(C3=CC=CC=C3)N(C4=CC(N)=CC=C24)CCCCCC[P+](C5=CC=CC=C5)(C6=CC=CC=C6)C7=CC=CC=C7)=C1.[I-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for Mito-HE
References for Mito-HE
References are publications that support the biological activity of the product.
Rhee et al (2020) Modeling secondary iron overload cardiomyopathy with human induced pluripotent stem cell-derived cardiomyocytes. Cell Rep. 32 107886 PMID: 32668256
Renaudin et al (2021) BRCA2 deficiency reveals that oxidative stress impairs RNaseH1 function to cripple mitochondrial DNA maintenance. Cell Rep. 36 109478 PMID: 34348152
Mukhopadhyay et al (2007) Simple quantitative detection of mitochondrial superoxide production in live cells. Biochem.Biophys.Res.Commun. 358 203 PMID: 17475217
Mukhopadhyay et al (2007) Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy. Nat.Protoc. 2 2295 PMID: 17853886
Robinson et al (2006) Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc.Natl.Acad.Sci.U.S.A. 103 15038 PMID: 17015830
Chen et al (2020) Trilobatin protects against Aβ 25-35-induced hippocampal HT22 cells apoptosis through mediating ROS/p38/caspase 3-dependent pathway. Front.Pharmacol. 11 584 PMID: 32508629
Chung et al (2016) Parkin and PINK1 patient iPSC-derived midbrain dopamine neurons exhibit mitochondrial dysfunction and α-synuclein accumulation. Stem Cell Rep. 7 664 PMID: 27641647
If you know of a relevant reference for Mito-HE, please let us know.
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Keywords: Mito-HE, Mito-HE supplier, MitoHE, red, mitochondrial, marker, stain, mitotracker, mitochondria, MitoSOX, MitoSox, superoxide, indicator, Fluorescent, Cell, Indicators, and, Sensors, Other, Mitochondria, Dyes, Probes, 7641, Tocris Bioscience
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Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals