Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewMitoMark Green I
Discontinued Product
6444 has been discontinued.
View all Other Mitochondria Dyes and Probes products.
Description: Green fluorescent mitochondrial stain; cell permeable
Chemical Name: 2-[3-[5,6-Dichloro-1,3-bis[[4-(chloromethyl)phenyl]methyl]-1,3-dihydro-2H-benzimidazol-2-ylidene]-1-propen-1-yl]-3-methylbenzoxazolium chloride
Purity: ≥85% (HPLC)
Biological Activity for MitoMark Green I
MitoMark Green I is a green fluorescent mitochondrial stain. Localizes to mitochondria regardless of mitochondrial membrane potential. Excitation/emission maxima λ ~ 490/516 nm.
It is recommended to prepare stock solutions in DMSO.
Scientific Data
Fluorescence Imaging
View Larger
View Larger
Applications in HeLa cells for MitoMark Green I. Application of MitoMark Green I: MitoMark Green I was diluted in HBSS and applied to live HeLa cells at a concentration of 200 nM. HeLa cells were incubated for 30 minutes at 37°C. After incubation was complete, MitoMark Green I dilutions were washed off, and fresh HBSS was applied to the cells. Cells were not fixed because MitoMark Green I staining is not retained well after fixation (photobleach). Live cell staining is optimal for MitoMark Green I.
Optical Data for MitoMark Green I
| Emission Color | Green |
|---|---|
| λabs | 490 nm |
| λem | 516 nm |
| Extinction Coefficient (ε) | 119000 M-1cm-1 |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data for MitoMark Green I
| M. Wt | 671.87 |
| Formula | C34H28Cl5N3O |
| Storage | Store at -20°C |
| Purity | ≥85% (HPLC) |
| CAS Number | 201860-17-5 |
| PubChem ID | 77913543 |
| InChI Key | RUVJFMSQTCEAAB-UHFFFAOYSA-M |
| Smiles | ClC1=CC2=C([N+](CC3=CC=C(CCl)C=C3)=C(/C=C/C=C4N(C)C5=CC=CC=C5O\4)N2CC6=CC=C(CCl)C=C6)C=C1Cl.[Cl-] |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Product Datasheets for MitoMark Green I
References for MitoMark Green I
References are publications that support the biological activity of the product.
Garner et al (1997) Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biol.Reprod. 57 1401 PMID: 9408246
View Related Products by Target
Keywords: MitoMark Green I, MitoMark Green I supplier, MitoMark, green, mitochondrial, marker, stain, mitotracker, mitochondria, Organelle, and, Cell, Structure, Stains, Labels, Flow, Cytometry, Other, Mitochondria, Dyes, Probes, 6444, Tocris Bioscience
Citations for MitoMark Green I
Citations are publications that use Tocris products.
Currently there are no citations for MitoMark Green I.
Reviews for MitoMark Green I
There are currently no reviews for this product. Be the first to review MitoMark Green I and earn rewards!
Have you used MitoMark Green I?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
Fluorescent Dyes and Probes Research Product Guide
This product guide provides a background to the use of Fluorescent Dyes and Probes, as well as a comprehensive list of our:
- Fluorescent Dyes, including dyes for flow cytometry
- Fluorescent Probes and Stains, including our new MitoBrilliantTM mitochondria stains
- Tissue Clearing Kits and Reagents
- Aptamer-based RNA Imaging Reagents
- Fluorescent Probes for Imaging Bacteria
- TSA VividTM Fluorophore Kits
- TSA Reagents for Enhancing IHC, ICC & FISH Signals