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Submit ReviewML SA1
Description: Activator of TRPML channels
Chemical Name: 2-[2-(3,4-Dihydro-2,2,4-trimethyl-1(2H)-quinolinyl)-2-oxoethyl]-1H-isoindole-1,3(2H)-dione
Purity: ≥98% (HPLC)
Biological Activity for ML SA1
ML SA1 is an activator of TRPML channels (TRPML1, 2 and 3); does not activate TRPM2, TRPV2, TRPV3, TRPC6 or TRPA1 channels. Induces TRPML-mediated Ca2+ release from lysosomes; activity corrects trafficking defects and reduces cholesterol accumulation in Niemann-Pick type C macrophages. Activation of TRPML1 by ML SA1 causes membrane accumulation of aquaporin-3 and -5, and significant depolymerization of the actin cytoskeleton, in human lymphatic endothelial cells (HLECs).
Compound Libraries for ML SA1
ML SA1 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.
Technical Data for ML SA1
| M. Wt | 362.42 |
| Formula | C22H22N2O3 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 332382-54-4 |
| PubChem ID | 2880983 |
| InChI Key | KDDHBJICVBONAX-UHFFFAOYSA-N |
| Smiles | CC2CC(C)(C)N(C(CN3C(C(C=CC=C4)=C4C3=O)=O)=O)C1=CC=CC=C12 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for ML SA1
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.25 | 20 |
Preparing Stock Solutions for ML SA1
The following data is based on the product molecular weight 362.42. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 13.8 mL | 68.98 mL | 137.96 mL |
| 1 mM | 2.76 mL | 13.8 mL | 27.59 mL |
| 2 mM | 1.38 mL | 6.9 mL | 13.8 mL |
| 10 mM | 0.28 mL | 1.38 mL | 2.76 mL |
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Product Datasheets for ML SA1
References for ML SA1
References are publications that support the biological activity of the product.
Shen et al (2012) Lipid storage disorders block lysosomal trafficking by inhibiting TRP channel and calcium release. Nat.Commun. 3 731 PMID: 22415822
Weiss et al (2012) Cross-talk between TRPML1 channel, lipids and lysosomal storage diseases. Commun.Integr.Biol. 5 111 PMID: 22808310
Yang (2024) TRPML1 acts as a predisposing factor in lymphedema development by regulating the subcellular localization of aquaporin-3, -5. PLoS One 19 e0310653 PMID: 39637010
If you know of a relevant reference for ML SA1, please let us know.
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Keywords: ML SA1, ML SA1 supplier, MLSA1, trpml, transient, receptor, potential, channels, mucolipin, TRPML1, TRPML2, TRPML3, lysosomal, lysosome, storage, diseases, TRPML, 4746, Tocris Bioscience
9 Citations for ML SA1
Citations are publications that use Tocris products. Selected citations for ML SA1 include:
Yang et al (2024) TRPML1 acts as a predisposing factor in lymphedema development by regulating the subcellular localization of aquaporin-3, -5. PLoS One 19 e0310653 PMID: 39637010
Wim et al (2023) Phospholipase D3 degrades mitochondrial DNA to regulate nucleotide signaling and APP metabolism. Nat Commun 14 2847 PMID: 37225734
Wei et al (2021) Berbamine inhibits Japanese encephalitis virus (JEV) infection by compromising TPRMLs-mediated endolysosomal trafficking of low-density lipoprotein receptor (LDLR). Emerg Microbes Infect 10 1257-1271 PMID: 34102949
Steven A et al (2022) Lysosomes and the pathogenesis of merosin-deficient congenital muscular dystrophy. Hum Mol Genet 31 733-747 PMID: 34568901
Haoxing et al (2022) A protocol to measure lysosomal Zn2+ release through a genetically encoded Zn2+ indicator. STAR Protoc 3 101453 PMID: 35707683
Yan et al (2022) LAMTOR1 inhibition of TRPML1-dependent lysosomal calcium release regulates dendritic lysosome trafficking and hippocampal neuronal function. EMBO J 41 e108119 PMID: 35099830
Jacques et al (2022) Microtubules restrict F-actin polymerization to the immune synapse via GEF-H1 to maintain polarity in lymphocytes. Elife 11 PMID: 36111670
Ana-Maria et al (2020) Trpml controls actomyosin contractility and couples migration to phagocytosis in fly macrophages. J Cell Biol 219 PMID: 31940424
Fine et al (2018) Structural basis for PtdInsP2-mediated human TRPML1 regulation. Nat.Commun. 9 4192 PMID: 30305615
Do you know of a great paper that uses ML SA1 from Tocris? Please let us know.
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
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Autophagy Scientific Review
Written by Patricia Boya and Patrice Codogno, this review summarizes the molecular mechanisms, physiology and pathology of autophagy. The role of autophagy in cell death and its links to disease are also discussed. Compounds available from Tocris are listed.
Autophagy Poster
Autophagy is a cellular process used by cells for degradation and recycling. Written by Patricia Boya and Patrice Codogno, this poster summarizes the molecular machinery, physiology and pathology of autophagy. Compounds available from Tocris are listed.