Submit a Review & Earn an Amazon Gift Card
You can now submit reviews for your favorite Tocris products. Your review will help other researchers decide on the best products for their research. Why not submit a review today?!
Submit ReviewNBC 6
Description: NLRP3 inflammasome inhibitor
Chemical Name: (T4)-[2-Acetyl-4,4,4-trichloro-3-(imino-κN)butanamidito-κO]diphenylboron
Purity: ≥98% (HPLC)
Biological Activity for NBC 6
NBC 6 is a NLRP3 inflammasome inhibitor (IC50 = 574 nM). Inhibits nigericin-induced inflammasome activation in THP-1 cells and Imiquimod (Cat. No. 3700) induced IL-1β release from LPS-primed bone marrow-derived macrophages (BMDMs).
Licensing Information
Sold under license from the University of Manchester.
Technical Data for NBC 6
| M. Wt | 409.5 |
| Formula | C18H16BCl3N2O2 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 2068818-02-8 |
| PubChem ID | 145237739 |
| InChI Key | VKECODDKRKQJNM-UHFFFAOYSA-N |
| Smiles | CC(C1=C(C(Cl)(Cl)Cl)N[B-](C2=CC=CC=C2)(C3=CC=CC=C3)[O+]=C1N)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for NBC 6
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 40.95 | 100 | |
| ethanol | 2.05 | 5 |
Preparing Stock Solutions for NBC 6
The following data is based on the product molecular weight 409.5. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.44 mL | 12.21 mL | 24.42 mL |
| 5 mM | 0.49 mL | 2.44 mL | 4.88 mL |
| 10 mM | 0.24 mL | 1.22 mL | 2.44 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.49 mL |
Molarity Calculator
Molarity Calculator
Calculate the mass, volume, or concentration required for a solution.
Reconstitution Calculator
Reconstitution Calculator
Dilution Calculator
Dilution Calculator
Calculate the dilution required to prepare a stock solution.
Product Datasheets for NBC 6
References for NBC 6
References are publications that support the biological activity of the product.
Baldwin et al (2017) Boron-based inhibitors of the NLRP3 inflammasome. Cell Chem.Biol. 24 1321 PMID: 28943355
If you know of a relevant reference for NBC 6, please let us know.
View Related Products by Target
View Related Products by Product Action
Keywords: NBC 6, NBC 6 supplier, NBC6, inflammasome, inhibitors, inhibits, NLRP3, Inflammasomes, 6925, Tocris Bioscience
Citations for NBC 6
Citations are publications that use Tocris products.
Currently there are no citations for NBC 6. Do you know of a great paper that uses NBC 6 from Tocris? Please let us know.
Reviews for NBC 6
Average Rating: 5 (Based on 1 Review.)
Have you used NBC 6?
Submit a review and receive an Amazon gift card.
$50/€35/£30/$50CAN/¥300 Yuan/¥5000 Yen for first to review with an image
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
NBC 6 can efficiently block pyroptosis induction.
By
Xiaogang Wang on 06/12/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: THP-1 cells
We ordered this product, combining with another pyroptosis inhibitor NSA, to confirm the induction of pyroptosis by staphylococcal virulence factors. We have shown that NSA significantly inhibited the release of LDH by THP-1 cells treated with staphylococcal products. To confirm that tested staphylococcal product induces pyroptosis, we also used 20 uM NBC 6 to pretreat THP-1 cells before incubating with staphylococcal product. Similarly, treatment of THP-1 cells with NBC 6 also significantly decreased the release of LDH in THP-1 cells treated by staphylococcal product.
