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Submit ReviewNexinhib20
Description: Rab27 inhibitor; inhibits neutrophil exocytosis; active in vivo
Chemical Name: 4,4-Dimethyl-1-(3-nitrophenyl)-2-(1H-1,2,4-triazol-1-yl)-1-penten-3-one
Purity: ≥98% (HPLC)
Biological Activity for Nexinhib20
Nexinhib20 is a Rab27 inhibitor (IC50 = 330 nM); inhibits Rab27a-JFC1 binding. Selectively inhibits neutrophil exocytosis, with no significant effect on other important innate immune responses including phagocytosis. Suppresses extracellular production of superoxide anion and cytochrome b plasma membrane expression. Also inhibits upregulation of adhesion molecules CD11b and CD66b. Displays in vivo anti-inflammatory activity.
Compound Libraries for Nexinhib20
Nexinhib20 is also offered as part of the Tocriscreen 2.0 Max. Find out more about compound libraries available from Tocris.
Technical Data for Nexinhib20
| M. Wt | 300.31 |
| Formula | C15H16N4O3 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 331949-35-0 |
| PubChem ID | 5715188 |
| InChI Key | VKSOIYNNOFGGES-JYRVWZFOSA-N |
| Smiles | CC(C)(C(/C(N1C=NC=N1)=C/C2=CC([N+]([O-])=O)=CC=C2)=O)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Nexinhib20
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 30.03 | 100 |
Preparing Stock Solutions for Nexinhib20
The following data is based on the product molecular weight 300.31. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.33 mL | 16.65 mL | 33.3 mL |
| 5 mM | 0.67 mL | 3.33 mL | 6.66 mL |
| 10 mM | 0.33 mL | 1.66 mL | 3.33 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.67 mL |
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Product Datasheets for Nexinhib20
References for Nexinhib20
References are publications that support the biological activity of the product.
Johnson et al (2016) Identification of neutrophil exocytosis inhibitors (Nexinhibs), small molecule inhibitors of neutrophil exocytosis and inflammation: druggability of the small GTPase Rab27a. J.Biol.Chem. 291 25965 PMID: 27702998
If you know of a relevant reference for Nexinhib20, please let us know.
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Keywords: Nexinhib20, Nexinhib20 supplier, Rab27, inhibitors, inhibits, Rab27a-JFC1, binding, neutrophil-specific, exocytosis, anti-inflammatory, Nexinhib, 20, Other, Small, Monomeric, GTPases, 6089, Tocris Bioscience
1 Citation for Nexinhib20
Citations are publications that use Tocris products. Selected citations for Nexinhib20 include:
Jong-Hyuk et al (2020) Inhibition of Rab27a and Rab27b Has Opposite Effects on the Regulation of Hair Cycle and Hair Growth. Int J Mol Sci 21 PMID: 32784729
Do you know of a great paper that uses Nexinhib20 from Tocris? Please let us know.
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