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Submit ReviewNifedipine
Description: CaV1.x blocker
Chemical Name: 1,4-Dihydro-2,6-dimethyl-4-(2-nitrophenyl)-3,5-pyridinedicarboxylic acid dimethyl ester
Purity: ≥98% (HPLC)
Biological Activity for Nifedipine
Nifedipine is a L-type calcium channel blocker.
Technical Data for Nifedipine
| M. Wt | 346.34 |
| Formula | C17H18N2O6 |
| Storage | Desiccate at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 21829-25-4 |
| PubChem ID | 4485 |
| InChI Key | HYIMSNHJOBLJNT-UHFFFAOYSA-N |
| Smiles | COC(=O)C1=C(C)NC(C)=C(C1C1=CC=CC=C1[N+]([O-])=O)C(=O)OC |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Nifedipine
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 34.63 | 100 |
Preparing Stock Solutions for Nifedipine
The following data is based on the product molecular weight 346.34. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.89 mL | 14.44 mL | 28.87 mL |
| 5 mM | 0.58 mL | 2.89 mL | 5.77 mL |
| 10 mM | 0.29 mL | 1.44 mL | 2.89 mL |
| 50 mM | 0.06 mL | 0.29 mL | 0.58 mL |
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Product Datasheets for Nifedipine
References for Nifedipine
References are publications that support the biological activity of the product.
Stork and Cocks (1994) Pharmacological reactivity of human epicardial coronary arteries: phasic and tonic responses to vasoconstrictor agents differentiated by nifed. Br.J.Pharmacol. 113 1093 PMID: 7889259
Merck Index 12 6617
If you know of a relevant reference for Nifedipine, please let us know.
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Keywords: Nifedipine, Nifedipine supplier, Ca2+, channel, blockers, L-type, Calcium, CaV, Channels, voltage-gated, voltage-dependent, Cav1.x, 1075, Tocris Bioscience
⚠ WARNING: This product can expose you to chemicals including Nifedipine, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov
37 Citations for Nifedipine
Citations are publications that use Tocris products. Selected citations for Nifedipine include:
Kraidith et al (2016) Hepcidin and 1,25(OH)2D3 effectively restore Ca2+ transport in β-thalassemic mice: reciprocal phenomenon of Fe2+ and Ca2+ absorption. Am J Physiol Endocrinol Metab 311 E214 PMID: 27245334
Lee et al (2015) N-MthD.-aspartate receptors mediate activity-dependent down-regulation of potassium channel genes during the expression of homeostatic intrinsic plasticity. J Neurosci 8 4 PMID: 25599691
Villalba et al (2007) Contribution of both Ca2+ entry and Ca2+ sensitization to the alpha1-adrenergic vasoconstriction of rat penile small arteries. Am J Physiol Heart Circ Physiol 292 H1157 PMID: 17085536
Ris and Godaux (2007) Synapse specificity of long-term potentiation breaks down with aging. Learn Mem 14 185 PMID: 17351142
Florea et al (2017) Calcium-regulatory proteins as modulators of chemotherapy in human neuroblastoma. Oncotarget 8 22876 PMID: 28206967
Abe et al (2011) The Etv1/Er81 transcription factor orchestrates activity-dependent gene regulation in the terminal maturation program of cerebellar granule cells. Mol Brain 108 12497 PMID: 21746923
Landstrom et al (2011) Junctophilin-2 expression silencing causes cardiocyte hypertrophy and abnormal intracellular calcium-handling. FEMS Immunol Med Microbiol 4 214 PMID: 21216834
Xu et al (2011) Mechanistic distinction between activation and inhibition of (Na(+)+K(+))-ATPase-mediated Ca2+ influx in cardiomyocytes. Mol Cells 406 200 PMID: 21303662
Hanlon et al (2016) Modulation of breast cancer cell viability by a cannabinoid receptor 2 agonist, JWH-015, is calcium dependent. Breast Cancer (Dove Med Press) 8 59 PMID: 27186076
Zemkova et al (2011) NE causes a biphasic change in mammalian pinealocye membrane potential: role of alpha1B-adrenoreceptors, phospholipase C, and Ca2+. Endocrinology 152 3842 PMID: 21828176
Luoma et al (2011) Progesterone inhibition of voltage-gated calcium channels is a potential neuroprotective mechanism against excitotoxicity. Steroids 76 845 PMID: 21371490
Mauger et al (2016) Targeted Intron Retention and Excision for Rapid Gene Regulation in Response to Neuronal Activity. Neuron 92 1266 PMID: 28009274
Pigott and Garthwaite (2016) Nitric Oxide Is Required for L-Type Ca(2+) Channel-Dependent Long-Term Potentiation in the Hippocampus. Front Synaptic Neurosci 8 17 PMID: 27445786
Shin et al (2008) Dendritic glutamate release produces autocrine activation of mGluR1 in cerebellar Purkinje cells. Proc Natl Acad Sci U S A 105 746 PMID: 18174329
Soriano et al (2008) Specific targeting of pro-death NMDA receptor signals with differing reliance on the NR2B PDZ ligand. Biochem Biophys Res Commun 28 10696 PMID: 18923045
Bellono (2017) Molecular basis of ancestral vertebrate electroreception. Nature 543 391 PMID: 28264196
Kim and Rhim (2011) Effects of amyloid-β peptides on voltage-gated L-type Ca(V)1.2 and Ca(V)1.3 Ca(2+) channels. Proc Natl Acad Sci U S A 32 289 PMID: 21822937
Ciappelloni et al (2019) Aquaporin-4 Surface Trafficking Regulates Astrocytic Process Motility and Synaptic Activity in Health and Autoimmune Disease. Cell Rep 27 3860 PMID: 31242419
Ludwar et al (2009) Two distinct mechanisms mediate potentiating effects of depolarization on synaptic transmission. J Neurophysiol 102 1976 PMID: 19605611
Hirasawa (2003) Nifedipine facilitates neurotransmitter release independently of calcium channels. Proc Natl Acad Sci U S A 100 6139 PMID: 12714685
Kim et al (2012) Decreased afferent excitability contributes to synaptic depression during high-frequency stimulation in hippocampal area CA1. J Neurophysiol 108 1965 PMID: 22773781
Yang et al (2012) A critical role for protein tyrosine phosphatase nonreceptor type 5 in determining individual susceptibility to develop stress-related cognitive and morphological changes. J Neurosci 32 7550 PMID: 22649233
Kato et al (2009) Non-Hebbian synaptic plasticity induced by repetitive postsynaptic action potentials. J Neurosci 29 11153 PMID: 19741122
Phelps et al (2022) The Dependence of Urinary Bladder Responses on Extracellular Calcium Varies Between Muscarinic, Histamine, 5-HT (Serotonin), Neurokinin, Prostaglandin, and Angiotensin Receptor Activation Front Physiol 13 841181 PMID: 35431993
Lopez et al (2022) Ketamine exerts its sustained antidepressant effects via cell-type-specific regulation of Kcnq2. Neuron 110 2283 PMID: 35649415
Thiagarajan et al (2005) Adaptation to synaptic inactivity in hippocampal neurons. Neuron 47 725 PMID: 16129401
Wang and Mattson (2014) L-type Ca2+ currents at CA1 synapses, but not CA3 or dentate granule neuron synapses, are increased in 3xTgAD mice in an age-dependent manner. Neurobiol Aging 35 88 PMID: 23932880
Wieland et al (2006) Efficient intracellular multiplication of Legionella pneumophila in human monocytes requires functional host cell L-type calcium channels. J Neurosci 47 296 PMID: 16831218
Ring et al (2012) GABA maintains the proliferation of progenitors in the developing chick ciliary marginal zone and non-pigmented ciliary epithelium. PLoS One 7 e36874 PMID: 22590629
Caiati et al (2012) Developmental regulation of CB1-mediated spike-time dependent depression at immature mossy fiber-CA3 synapses. Sci Rep 2 285 PMID: 22368777
Kyriazis et al (2010) Stress-induced switch in Numb isoforms enhances Notch-dependent expression of subtype-specific transient receptor potential channel. J Biol Chem 285 6811 PMID: 20038578
Potier et al (2010) Contribution of the d-Serine-Dependent Pathway to the Cellular Mechanisms Underlying Cognitive Aging. Front Aging Neurosci 2 1 PMID: 20552041
Maier et al (2010) Sustained glutamate receptor activation down-regulates GABAB receptors by shifting the balance from recycling to lysosomal degradation. J Biol Chem 285 35606 PMID: 20826795
Large et al (2010) Ionic currents in intimal cultured synoviocytes from the rabbit. Am J Physiol Cell Physiol 299 C1180 PMID: 20720182
Fujino et al (2003) Regulation of cpg15 by signaling pathways that mediate synaptic plasticity. Mol Cell Neurosci 24 538 PMID: 14664806
Armstrong and Drapeau (2013) Calcium channel agonists protect against neuromuscular dysfunction in a genetic model of TDP-43 mutation in ALS. Circ Heart Fail 33 1741 PMID: 23345247
Kaufman et al (2012) Opposing roles of synaptic and extrasynaptic NMDA receptor signaling in cocultured striatal and cortical neurons. Mol Brain 32 3992 PMID: 22442066
Do you know of a great paper that uses Nifedipine from Tocris? Please let us know.
Reviews for Nifedipine
Average Rating: 4.8 (Based on 5 Reviews.)
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breast cancer cell viability by a cannabinoid receptor 2 agonist.
By
Anonymous on 03/15/2020
Assay Type: In Vitro
Species: Human
Cell Line/Tissue: MCF-7
We used all these compounds which were dissolved in DMSO and added to fresh culture media. Concentration reference paper PMID: 27186076
The pharmacological inhibitors for electrophysiology assay.
By
Anonymous on 02/28/2020
Assay Type: In Vitro
Species: Other
The following concentrations we were used:1 μM Bay K, 10 μM nifedipine, 10 μM nimodipine, 300 nM omega -agatoxin, 1 μM omega -conotoxin, 5 μM mibefradil, 1 μM tetrodotoxin, 1 μM charybdotoxin, 100 nM iberiotoxin, 10 μM NS11021.
Blocks L type calcium entry in neurons.
By
Karthik krishnamurthy on 11/02/2018
Assay Type: In Vitro
Species: Rat
Cell Line/Tissue: spinal cord neurons
Effectively blocks KCL mediated L type calcium entry in neurons. Tested in spinal cord neuron cultures at a concentration of 10 micromolar.
Nifedipine blocks L-type Ca2+ current in primary cardiomyoytes.
By
Chintan Koyani on 10/23/2018
Assay Type: In Vitro
Species: Other
Cell Line/Tissue: Primary ventricular cardiomyocytes
L-type Ca2+ current of guinea pig cardiomyocytes before and after nifedipine (1 µM) superfusion for 5 min. Compared to time-matched rundown, nifedipine significantly blocked L-type Ca2+ current in cardiomyocytes
Applied to GI smooth muscle in tissue bath.
By
John Broad on 01/05/2018
Assay Type: In Vitro
Species: Human
Applied to human GI smooth muscle
Effective calcium channel blocker