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Submit ReviewNS 3728
Description: VRAC blocker
Alternative Names: Endovion
Chemical Name: N-[3,5-bis(Trifluoromethyl)phenyl]-N'-[4-bromo-2-(2H-tetrazol-5-yl)phenyl]urea
Purity: ≥98% (HPLC)
Biological Activity for NS 3728
NS 3728 is a volume-regulated anion channel (VRAC) blocker (IC50 = 460 nM). Blocks red blood cell chloride conductance (IC50 = 0.6 μM). NS 3728 also inhibits Ca2+-activated Cl- current (CaCC) by anoctamin (ANO) in an apparently voltage-dependent manner (IC50 against ANO1 = 2.1 and 0.7 μM at -95 and +50 mV, respectively).
Technical Data for NS 3728
| M. Wt | 495.18 |
| Formula | C16H9BrF6N6O |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 265646-85-3 |
| PubChem ID | 10005966 |
| InChI Key | AEFYFGMSRKDXHZ-UHFFFAOYSA-N |
| Smiles | FC(F)(C1=CC(C(F)(F)F)=CC(NC(NC2=C(C3=NN=NN3)C=C(C=C2)Br)=O)=C1)F |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for NS 3728
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 49.52 | 100 | |
| ethanol | 24.76 | 50 |
Preparing Stock Solutions for NS 3728
The following data is based on the product molecular weight 495.18. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.02 mL | 10.1 mL | 20.19 mL |
| 5 mM | 0.4 mL | 2.02 mL | 4.04 mL |
| 10 mM | 0.2 mL | 1.01 mL | 2.02 mL |
| 50 mM | 0.04 mL | 0.2 mL | 0.4 mL |
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Product Datasheets for NS 3728
References for NS 3728
References are publications that support the biological activity of the product.
Helix et al (2003) Inhibition of the endogenous volume-regulated anion channel (VRAC) in HEK293 cells by acidic di-aryl-ureas. J.Membr.Biol. 196 83 PMID: 14724745
Kjaer et al (2009) Chloride channel blockers inhibit iNOS expression and NO production in IFNgamma-stimulated microglial BV2 cells. Brain Res. 1281 24 PMID: 19446535
Godwin et al (2019) E-cadherin-deficient cells have synthetic lethal vulnerabilities in plasma membrane organisation, dynamics and function. Gastric Cancer 22 273 PMID: 30066183
Sauter et al (2015) ANO1 (TMEM16A) in pancreatic ductal adenocarcinoma (PDAC). Pflugers Arch. 467 1495 PMID: 25163766
If you know of a relevant reference for NS 3728, please let us know.
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Keywords: NS 3728, NS 3728 supplier, NS3728, volume-regulated, anion, channel, VRAC, blockers, Endovion, Other, Chloride, Channels, 6973, Tocris Bioscience
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