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Description: Thioredoxin-1 inhibitor
Alternative Names: IV-2
Chemical Name: 2-[(1-Methylpropyl)dithio]-1H-imidazole
Purity: ≥99% (HPLC)
Biological Activity for PX 12
PX 12 is a thioredoxin-1 (Trx-1) inhibitor; irreversibly inactivates Trx-1 as a substrate for reduction by thioredoxin reductase (TR); competitively inhibits the reduction of Trx-1 by TR (Ki = 30.8 μM). Attenuates expression of HIF-1α, VEGF and iNOS (IC50 values are 7.2, 10.4 and 18.1 μM respectively). Exhibits antitumor activity; directly inhibits tubulin in vitro and decreases tumor microvessel density in vivo.
Technical Data for PX 12
| M. Wt | 188.31 |
| Formula | C7H12N2S2 |
| Storage | Store at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 141400-58-0 |
| PubChem ID | 219104 |
| InChI Key | BPBPYQWMFCTCNG-UHFFFAOYSA-N |
| Smiles | CC(CC)SSC1=NC=CN1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for PX 12
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 18.83 | 100 |
Preparing Stock Solutions for PX 12
The following data is based on the product molecular weight 188.31. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 5.31 mL | 26.55 mL | 53.1 mL |
| 5 mM | 1.06 mL | 5.31 mL | 10.62 mL |
| 10 mM | 0.53 mL | 2.66 mL | 5.31 mL |
| 50 mM | 0.11 mL | 0.53 mL | 1.06 mL |
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Product Datasheets for PX 12
References for PX 12
References are publications that support the biological activity of the product.
Kirkpatrick et al (1998) Mechanisms of inhibition of the thioredoxin growth factor system by antitumor 2-imidazolyl disulfides. Biochem.Pharmacol. 55 987 PMID: 9605422
Welsh et al (2003) The thioredoxin redox inhibitors 1-methylpropyl 2-imidazolyl disulfide and pleurotin inhibit hypoxia-induced factor 1α and vascular endothelial growth factor formation. Mol.Cancer.Ther. 2 235 PMID: 12657718
Jordan et al (2005) The thioredoxin-1 inhibitor 1-methylpropyl 2-imidazolyl disulfide (PX-12) decreases vascular permeability in tumor xenografts monitored by dynamic contrast enhanced magnetic resonance imaging. Clin.Cancer Res. 11 529 PMID: 15701837
If you know of a relevant reference for PX 12, please let us know.
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Keywords: PX 12, PX 12 supplier, px12, thioredoxin-1, trx-1, inhibitors, inhibits, HIF-1alpha, HIF-1a, HIF-1α, IV-2, antiangiogenics, Antiangiogenics, Hypoxia, Inducible, Factors, Thioredoxin, Reductases, 2954, Tocris Bioscience
2 Citations for PX 12
Citations are publications that use Tocris products. Selected citations for PX 12 include:
Shin et al (2013) PX-12-induced HeLa cell death is associated with oxidative stress and GSH depletion. Oncol Lett 6 1804 PMID: 24260080
Zhu et al (2012) BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells. Cell Death Differ 31 31 PMID: 22463935
Do you know of a great paper that uses PX 12 from Tocris? Please let us know.
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Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
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