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Submit ReviewRhosin hydrochloride
Description: Rho inhibitor
Chemical Name: D-Tryptophan (2E)-2-(6-quinoxalinylmethylene)hydrazide hydrochloride
Purity: ≥98% (HPLC)
Biological Activity for Rhosin hydrochloride
Rhosin hydrochloride is a Rho GTPase inhibitor (Kd = 354 nM). Inhibits binding of RhoA to guanine nucleotide exchange factors (GEFs) including LARG, DBL, LBC, p115 RhoGEF and PDZ RhoGEF. Has no effect on the binding of Rac1 or Cdc42 to their respective GEFs. Suppresses RhoA/C-mediated breast cancer cell migration and invasion in vitro. Also induces neurite outgrowth in synergy with NGF.
Technical Data for Rhosin hydrochloride
| M. Wt | 394.86 |
| Formula | C20H18N6O.HCl |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1281870-42-5 |
| PubChem ID | 44662615 |
| InChI Key | SFRGBDFQSLZYLF-GRYLRVQNSA-N |
| Smiles | N[C@@H](C(N/N=C/C3=CC=C(N=CC=N4)C4=C3)=O)CC2=CNC1=CC=CC=C12.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for Rhosin hydrochloride
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 7.9 | 20 with gentle warming | |
| DMSO | 39.49 | 100 |
Preparing Stock Solutions for Rhosin hydrochloride
The following data is based on the product molecular weight 394.86. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.53 mL | 12.66 mL | 25.33 mL |
| 5 mM | 0.51 mL | 2.53 mL | 5.07 mL |
| 10 mM | 0.25 mL | 1.27 mL | 2.53 mL |
| 50 mM | 0.05 mL | 0.25 mL | 0.51 mL |
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Product Datasheets for Rhosin hydrochloride
References for Rhosin hydrochloride
References are publications that support the biological activity of the product.
Shang et al (2013) Small-molecule inhibitors targeting G-protein-coupled Rho guanine nucleotide exchange factors. Proc.Natl.Acad.Sci.U.S.A. 110 3155 PMID: 23382194
Shang et al (2012) Rational design of small molecule inhibitors targeting RhoA subfamily Rho GTPases. Chem.Biol. 19 699 PMID: 22726684
If you know of a relevant reference for Rhosin hydrochloride, please let us know.
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Keywords: Rhosin hydrochloride, Rhosin hydrochloride supplier, Rho, GTPases, inhibitors, inhibits, guanine, nucleotide, exchange, factors, GEF, 5003, Tocris Bioscience
7 Citations for Rhosin hydrochloride
Citations are publications that use Tocris products. Selected citations for Rhosin hydrochloride include:
Thomas et al (2023) Unique osteogenic profile of bone marrow stem cells stimulated in perfusion bioreactor is Rho-ROCK-mediated contractility dependent. Bioeng Transl Med 8 e10509 PMID: 37206242
Wenming et al (2021) Statin-mediated disruption of Rho GTPase prenylation and activity inhibits respiratory syncytial virus infection. Commun Biol 4 1239 PMID: 34716403
Ginam et al (2021) β2-adrenergic receptor regulates ER-mitochondria contacts. Sci Rep 11 21477 PMID: 34728663
Ying et al (2019) The p75NTR Influences Cerebellar Circuit Development and Adult Behavior via Regulation of Cell Cycle Duration of Granule Cell Progenitors. J Neurosci 39 9119-9129 PMID: 31582529
Ariyani et al (2022) Gadolinium-based contrast agent accelerates the migration of astrocyte via integrin αvβ3 signaling pathway Sci.Rep 12 5850 PMID: 35393504
Keejung et al (2020) Protein Arginine Methyltransferase 1 (PRMT1) Selective Inhibitor, TC-E 5003, Has Anti-Inflammatory Properties in TLR4 Signaling. Int J Mol Sci 21 PMID: 32357521
Richard et al (2020) Nonionotropic Action of Endothelial NMDA Receptors on Blood-Brain Barrier Permeability via Rho/ROCK-Mediated Phosphorylation of Myosin. J Neurosci 40 1778-1787 PMID: 31953371
Do you know of a great paper that uses Rhosin hydrochloride from Tocris? Please let us know.
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