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Submit ReviewRuBi-Glutamate
Description: Caged glutamate; excited by visible wavelengths
Chemical Name: (bis(2,2'-Bipyridine-N,N')trimethylphosphine)-(S)-1-aminopropane-1,3-dicarboxylic acid ruthenium(2+) complex sodium hexafluorophosphate salt
Biological Activity for RuBi-Glutamate
RuBi-Glutamate is a ruthenium-bipyridine-trimethylphosphine caged glutamate that can be excited by visible wavelengths and releases glutamate after one- or two-photon excitation. Has high quantum efficiency and can be used at low concentrations, partly avoiding blockade of GABAergic transmission seen with other caged glutamate compounds. Displays high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics.
MNI-caged-L-glutamate (Cat. No. 1490) also available.
Licensing Information
Sold under licence from Columbia University
Technical Data for RuBi-Glutamate
| M. Wt | 970.54 |
| Formula | C28H32N5O4PRu.2NaPF6 |
| Storage | Store at -20°C |
| CAS Number | 2417096-44-5 |
| PubChem ID | 90488860 |
| InChI Key | SQQVSHWHIPVYSZ-UHFFFAOYSA-L |
| Smiles | [Na+].[Na+].[Ru++].CP(C)C.F[P-](F)(F)(F)(F)F.F[P-](F)(F)(F)(F)F.NC(CCC([O-])=O)C([O-])=O.C1=CN=C(C=C1)C1=CC=CC=N1.C1=CN=C(C=C1)C1=CC=CC=N1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for RuBi-Glutamate
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 19.41 | 20 |
Preparing Stock Solutions for RuBi-Glutamate
The following data is based on the product molecular weight 970.54. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 5.15 mL | 25.76 mL | 51.52 mL |
| 1 mM | 1.03 mL | 5.15 mL | 10.3 mL |
| 2 mM | 0.52 mL | 2.58 mL | 5.15 mL |
| 10 mM | 0.1 mL | 0.52 mL | 1.03 mL |
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Product Datasheets for RuBi-Glutamate
References for RuBi-Glutamate
References are publications that support the biological activity of the product.
Fino et al (2009) RuBi-Glutamate: two-photon and visible-light photoactivation of neurons and dendritic spines. Front.Neural Circuits 3 1 PMID: 19506708
Salierno et al (2010) A fast ruthenium polypyridine cage complex photoreleases glutamate with visible or IR light in one and two photon regimes. J.Inorg.Biochem. 104 418 PMID: 20060592
If you know of a relevant reference for RuBi-Glutamate, please let us know.
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Keywords: RuBi-Glutamate, RuBi-Glutamate supplier, Caged, Glutamate, mGluR, iGluR, Receptors, agonists, ionotropic, metabotropic, Miscellaneous, Compounds, 3574, Tocris Bioscience
6 Citations for RuBi-Glutamate
Citations are publications that use Tocris products. Selected citations for RuBi-Glutamate include:
Mitchell et al (2019) Probing Single Synapses via the Photolytic Release of Neurotransmitters Front Synaptic Neurosci 11 PMID: 31354469
Kazemipour et al (2019) Kilohertz frame-rate two-photon tomography. Nat Methods 16 778 PMID: 31363222
Roberto et al (2013) Ruthenium polypyridyl phototriggers: from beginnings to perspectives. Philos Trans A Math Phys Eng Sci 371 20120330 PMID: 23776299
Paz et al (2018) Inhibition of striatal cholinergic interneuron activity by the Kv7 opener retigabine and the nonsteroidal anti-inflammatory drug diclofenac. Neuropharmacology 137 309 PMID: 29758221
Blaesse et al (2015) μ-Opioid Receptor-Mediated Inhibition of Intercalated Neurons and Effect on Synaptic Transmission to the Central Amygdala. Neuroscience 35 7317 PMID: 25972162
Tazerart et al (2020) Spike-timing-dependent plasticity rule for single, clustered and distributed dendritic spines. Nat Commun 11 4276 PMID: 32848151
Do you know of a great paper that uses RuBi-Glutamate from Tocris? Please let us know.
Reviews for RuBi-Glutamate
Average Rating: 5 (Based on 1 Review.)
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By
Anonymous on 06/01/2020
Assay Type: Ex Vivo
Species: Mouse
Cell Line/Tissue: Periaqueductal gray
Recording from a DA neuron in the periaqueductal gray. A 6 x 6 grid was used to locally uncage Rubi-glutamate (300 micromolar) in an area surrounding the neuron. Representative trace showing the responses to each focal stimulation in current-clamp mode.
Light sensitive.
Literature in this Area
Tocris offers the following scientific literature in this area to showcase our products. We invite you to request* your copy today!
*Please note that Tocris will only send literature to established scientific business / institute addresses.
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