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Submit ReviewSAG 21k
Description: Hedgehog signaling activator; brain penetrant and orally bioavailable
Chemical Name: 3-Chloro-4,7-difluoro-N-[[2-methoxy-5-(4-pyridinyl)phenyl]methyl]-N-trans-4-(methylamino)cyclohexyl]benzo[b]thiophene-2-carboxamide
Purity: ≥98% (HPLC)
Biological Activity for SAG 21k
SAG 21k is a potent Hedgehog signaling activator (EC50 = 0.4 nM). Orally bioavailable and brain penetrant.
Compound Libraries for SAG 21k
SAG 21k is also offered as part of the Tocriscreen 2.0 Max and Tocriscreen Stem Cell Library. Find out more about compound libraries available from Tocris.
Technical Data for SAG 21k
| M. Wt | 556.07 |
| Formula | C29H28ClF2N3O2S |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 946002-48-8 |
| PubChem ID | 16678532 |
| InChI Key | YVIFQUJDZSAFKG-UHFFFAOYSA-N |
| Smiles | COC1=C(CN([C@@H]2CC[C@@H](NC)CC2)C(C3=C(Cl)C4=C(C(F)=CC=C4F)S3)=O)C=C(C5=CC=NC=C5)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Solubility Data for SAG 21k
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 55.61 | 100 | |
| ethanol | 11.12 | 20 |
Preparing Stock Solutions for SAG 21k
The following data is based on the product molecular weight 556.07. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.8 mL | 8.99 mL | 17.98 mL |
| 5 mM | 0.36 mL | 1.8 mL | 3.6 mL |
| 10 mM | 0.18 mL | 0.9 mL | 1.8 mL |
| 50 mM | 0.04 mL | 0.18 mL | 0.36 mL |
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Product Datasheets for SAG 21k
References for SAG 21k
References are publications that support the biological activity of the product.
Brunton et al (2009) Potent agonists of the Hedgehog signaling pathway. Bioorg.Med.Chem.Lett. 19 4308 PMID: 19500978
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Keywords: SAG 21k, SAG 21k supplier, SAG21k, Hedgehog, Hh, signaling, activators, activation, orally, bioavailable, brain, penetrant, potent, Signaling, 5282, Tocris Bioscience
3 Citations for SAG 21k
Citations are publications that use Tocris products. Selected citations for SAG 21k include:
M et al (2021) Hedgehog signaling promotes endoneurial fibroblast migration and Vegf-A expression following facial nerve injury. Brain Res 1751 147204 PMID: 33189691
Olivier et al (2022) Paraxial mesoderm organoids model development of human somites. Elife 11 PMID: 35088712
Norris et al (2023) Hedgehog signaling via its ligand DHH acts as cell fate determinant during skeletal muscle regeneration Nat Commun 14 3766 PMID: 37355632
Do you know of a great paper that uses SAG 21k from Tocris? Please let us know.
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Literature in this Area
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Stem Cell Research Product Guide
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