Home / Pharmacology / Enzymes / Dehydrogenases / Aldehyde Dehydrogenases / Aldehyde Dehydrogenase Inhibitors / WIN 18446

WIN 18446

Cat. No. 4736 4 Citations 1 Review Submit a Review Datasheet / COA / SDS
Pricing Availability   Qty
Description: ALDH1A2 inhibitor
Chemical Name: N,N'-1,8-Octanediylbis(2,2-dichloroacetamide)
Datasheet
Citations (4)
Reviews (1)
Biological Activity Technical Data Solubility Calculators Datasheets References

Biological Activity for WIN 18446

WIN 18446 is an inhibitor of aldehyde dehydrogenase 1a2 (ALDH1a2) (IC50 = 0.3 μM). Inhibits the biosynthesis of retinoic acid from retinol in neonatal and adult murine testis.

Technical Data for WIN 18446

M. Wt 366.11
Formula C12H20Cl4N2O2
Storage Store at +4°C
CAS Number 1477-57-2
PubChem ID 15134
InChI Key FAOMZVDZARKPFJ-UHFFFAOYSA-N
Smiles ClC(Cl)C(NCCCCCCCCNC(C(Cl)Cl)=O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility Data for WIN 18446

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 36.61 100

Preparing Stock Solutions for WIN 18446

The following data is based on the product molecular weight 366.11. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.73 mL 13.66 mL 27.31 mL
5 mM 0.55 mL 2.73 mL 5.46 mL
10 mM 0.27 mL 1.37 mL 2.73 mL
50 mM 0.05 mL 0.27 mL 0.55 mL

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Product Datasheets for WIN 18446

Certificate of Analysis / Product Datasheet
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Safety Datasheet

References for WIN 18446

References are publications that support the biological activity of the product.

Amory et al (2011) Suppression of spermatogenesis by bisdichloroacetyldiamines is mediated by inhibition of testicular retinoic acid biosynthesis. J.Androl. 32 111 PMID: 20705791

Hogarth et al (2011) Suppression of Stra8 expression in the mouse gonad by WIN 18,446. Biol.Reprod. 84 957 PMID: 21209416

If you know of a relevant reference for WIN 18446, please let us know.

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Keywords: WIN 18446, WIN 18446 supplier, WIN18446, spermatogenesis, inhibitors, inhibits, aldehyde, dehydrogenases, ALDH1a2, Aldehyde, Dehydrogenase, 4736, Tocris Bioscience

4 Citations for WIN 18446

Citations are publications that use Tocris products. Selected citations for WIN 18446 include:

Martine et al (2017) Ubiquitin Ligase Huwe1 Modulates Spermatogenesis by Regulating Spermatogonial Differentiation and Entry into Meiosis. Sci Rep 7 17759 PMID: 29259204

Barrionuevo et al (2016) Sox9 and Sox8 protect the adult testis from male-to-female genetic reprogramming and complete degeneration. Elife 5 PMID: 27328324

Ana-Maria et al (2022) Epithelial colonization by gut dendritic cells promotes their functional diversification. Immunity 55 129-144.e8 PMID: 34910930

Corinne et al (2020) PRDM9 activity depends on HELLS and promotes local 5-hydroxymethylcytosine enrichment. Elife 9 PMID: 33047671

Do you know of a great paper that uses WIN 18446 from Tocris? Please let us know.

Reviews for WIN 18446

Average Rating: 4 (Based on 1 Review.)

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Effectively inhibits human Aldh1a2.
By Anonymous on 09/13/2019
Assay Type: In Vitro
Species: Human

This was used to inhibit recombinant Aldh1a2 activity in a titration curve.

Win-18446 showed an IC50 value at approximately 15 uM in a cell-free reaction assay. Was dissolved in DMSO to 100 mM with no solubility issues.

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